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Name : leuS (CLH_2897) Accession : YP_001922274.1 Strain : Clostridium botulinum Alaska E43 Genome accession: NC_010723 Putative virulence/resistance : Unknown Product : leucyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG0495 EC number : 6.1.1.4 Position : 3074155 - 3076605 bp Length : 2451 bp Strand : + Note : leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl DNA sequence : ATGGAAAATTACAGTACTAAAATCGATGAAAAATGGCAAAAGATTTGGGAAGAAAATGAAACTTACAAATTTAACCCAGA AAATTTAGATAAGAAACTTTATACACTTGAAATGTTCTCTTATCCATCAGGAGCTCAATTACATGCTGGTCACTGGTTTA ACTACGGACCAACAGATTCATGGGCAAGACTTAAAAGAATGCAAGGTTATAATGTATTCCAACCAATGGGTTTTGATGCT TTTGGATTACCTGCTGAAAACTATGCAATAAAAACAGGCATACACCCTAAAGATTCTACATTTAAAAATATTGAAACTAT GGAAAAGCAATTAAAATCTATGGGTGCTATGTTTAATTGGGAAAATGAAGTTGTCACTTGTTCTCCTGATTACTATAAAT GGACTCAATGGTTATTCTTAAAACTTTATGAAAAAGGATTAGCTTACAGAAAAAAAGCTCCTGTTAATTGGTGTCCATCT TGTAATACGGTTTTAGCTAATGAGCAAGTAGTTGATGGCTTATGTGAAAGATGTGAATCTGAAGTTATTAAGAAAGATCT AACTCAATGGTTCTTCAAAATAACTGATTATGCTGATGAATTACTAGATAAATTAGATGGCTTAGATTGGCCAGAAAAAA CTAAATCTATGCAAAAACATTGGGTTGGAAGATCATATGGTGCACAAGTTACATTTAAAGTTAAAGATTCTAGCTTAGAA TTTGATGTATTTACTACAAGAGTTGATACTTTAAATGGTGTAACTTATGTTGTATTAGCACCAGAAAATAAATTAGTTGA CGAATTAACTCTTCCACAATATAAGGAAGCTGTTGAAAAATACAAAGAAGAAGCTAGCAAGCAATCTGATATAGAAAGAC AATCATCATCGAGAGAAAAAAGTGGTGTATTTACTGGATCATATGCAATAAATCCTATAAACGGAAAAGAAGTTCCTGTA TGGATTGCTGATTATGTATTAGCTACATATGGTACTGGTTGTGTTATGGCAGTTCCAGCACATGATGAAAGAGATTTCGC ATTTGCAAGTAAATTCAATTTACCAATAGAAAGAGTTATTACTGACAAACAAGATAACGAGCCAGCTCTACCATTCTGCG AACACGGAACACTTGTTAATTCAGGACAATTTGATGGTTTAACTACAGATGAAGCTAAAAAAGCTATTGTTTCTGAACTT GAAAAAGATGAACTTGGAAAAATGAAAGTTAACTTCAGATTAAGAGACTGGTTAGTCTCAAGACAAAGATATTGGGGAGC TCCAATTCCGGTTATATACTGTGATGAATGTGGAATAGTTCCAGTTCCTGAAAAAGACTTACCAGTAGAACTTCCTTATA ATGTTGAGTTTACTCCAGATGGTAAATCACCACTTGGAAAATGTGAAGAATTCATAAACACCACTTGTCCTCACTGTGGT AAACCTGCAAAAAGAGAAGCAGATACTTTAGATACATTTGTATGTTCTTCTTGGTATTATTTAAGATACCCTGATAACAA AAATACTGATGCACCTTTTGATCCTGAATTAATAAATAAAATGTTACCTGTAGATAAATATGTTGGTGGACCAGAGCATG CTTGTATGCATTTATTATATGCAAGATTTATAACTAAAGCATTAAGGGATATGGGTTACTTAAAATTTGATGAACCATTC TTAAGCTTAACTCACCAAGGATTAATCTTAGGACCAGACGGATTGAAAATGAGTAAATCAAAAGGAAATACAATCTCTCC AGATGATTACATTAAAGAATTTGGTGCTGATGTATTTAGAATGTACTTAATGTTTGGTTTTGCATATGTTGAAGGTGGAG CATGGAGTGATGATGGAGTTAAATCTGTTGCTAGATTTGTAGATAGAATTGAAAGAACTTTAGAATCATGCAGAGATATC ATTAACGCTTCAGAAAATATTAAGACTACTATAGATTCTGAAGAAAAAGACTTGAATTTCTGGAGACATACAGCTATTAA AGGTGTTAGCGAAGATGCAGAAAAAATGCAATTTAATACTGCAATAGCAAGACTTATGGAATTTACAAATGCTCTTCATA AATATATTCAAGCTGATACTAAAAATCCTAAATTCTTAAAAGAAACTGTAGTAGATTTCTTGAAATTATTAGCACCATTT GCTCCTCACTTTACTGAAGAACAATGGAACTTGTTAGGTCACAACTCAACTATATTTAATGAAAAATGGCCTGAATTTAA TCCAGCTGCTTTAGTTAAAGATGAAGTTGAAATAGCTATTCAAGTTAATGGTAAAATAAAAGCTAAAATTATGATACCAT CAAACTTAGATGAAGAAGGTATAAAAAAAGCTTCATTAGAAAATGAAACTATAAAAGCAAATACTGAAGGTAAAAATATA ATAAAAGTAATTGTTATAAAAGGTAGACTTGTTAATATAGTTGTAAAATAG Protein sequence : MENYSTKIDEKWQKIWEENETYKFNPENLDKKLYTLEMFSYPSGAQLHAGHWFNYGPTDSWARLKRMQGYNVFQPMGFDA FGLPAENYAIKTGIHPKDSTFKNIETMEKQLKSMGAMFNWENEVVTCSPDYYKWTQWLFLKLYEKGLAYRKKAPVNWCPS CNTVLANEQVVDGLCERCESEVIKKDLTQWFFKITDYADELLDKLDGLDWPEKTKSMQKHWVGRSYGAQVTFKVKDSSLE FDVFTTRVDTLNGVTYVVLAPENKLVDELTLPQYKEAVEKYKEEASKQSDIERQSSSREKSGVFTGSYAINPINGKEVPV WIADYVLATYGTGCVMAVPAHDERDFAFASKFNLPIERVITDKQDNEPALPFCEHGTLVNSGQFDGLTTDEAKKAIVSEL EKDELGKMKVNFRLRDWLVSRQRYWGAPIPVIYCDECGIVPVPEKDLPVELPYNVEFTPDGKSPLGKCEEFINTTCPHCG KPAKREADTLDTFVCSSWYYLRYPDNKNTDAPFDPELINKMLPVDKYVGGPEHACMHLLYARFITKALRDMGYLKFDEPF LSLTHQGLILGPDGLKMSKSKGNTISPDDYIKEFGADVFRMYLMFGFAYVEGGAWSDDGVKSVARFVDRIERTLESCRDI INASENIKTTIDSEEKDLNFWRHTAIKGVSEDAEKMQFNTAIARLMEFTNALHKYIQADTKNPKFLKETVVDFLKLLAPF APHFTEEQWNLLGHNSTIFNEKWPEFNPAALVKDEVEIAIQVNGKIKAKIMIPSNLDEEGIKKASLENETIKANTEGKNI IKVIVIKGRLVNIVVK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| leuS | YP_281511.2 | leucyl-tRNA synthetase | Not tested | Not named | Protein | 8e-168 | 45 |
