Gene Information

Name : lysS (xccb100_2141)
Accession : YP_001903545.1
Strain : Xanthomonas campestris B100
Genome accession: NC_010688
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 2476766 - 2478283 bp
Length : 1518 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACCGAACAGACCCCCACGCCGCAGCTCCCCGCCGACGAGAACAGCCTCATCGCCGAGCGGCGCGCGAAACTGGGCGC
GTTGCGCGGCCAGGGCATCGCCTATCCGAACGATTTCGTGCGCACCCACTTTGCCGGCGATCTGCAGGCCGAGTTCGCCG
AGGCCGAAACCTGGACTGCCGAAGCGCTGGAAGCAGCCGACCGCAGCGTCAAGCTGGCGGGCCGGTTGATGGCCAAGCGG
GTGATGGGCAAGGCCAGCTTCGCGCAGATCCAGGACGAGTCCGGGCGCGTGCAGTTGTTCCTGCAGGGCAATGTGCTGGG
CGATGCCTACACCGCCTTCAAGGGTTGGGACGTGGGCGACATCGTGGCGGTGGAGGGCGGGCTGACCCGTACCAAGACCG
GCGAGCTGTCGGTCAAGGCCAGTGCGCTGCGTTTGCTGACCAAGTCGCTGCGTCCGCTGCCGGACAAGTGGCATGGCCTG
TCGGACGTGGAGCAGCGCTATCGCCAGCGCTATGTGGACCTGATCGTGACCCCGGAAGCGCGCGAGGTCTTCATCAAGCG
CTCCAAGATCATCCGCGCCATCCGCGCCTGGCTGGACGCGCGCCGCTTCCTGGAAGTGGAAACGCCGATGATGCATTACA
TCCCCGGCGGCGCCACTGCCAAGCCGTTCACCACCCACCACAACGCACTGGATCTGGACCTGTACCTGCGCGTGGCCCCG
GAGCTGTACCTCAAGCGGCTGGTGGTCGGTGGGCTGGAGCGCGTCTACGAGATCAACCGCAATTTCCGCAACGAAGGCGT
GTCGACCCGGCACAACCCCGAGTTCACCATGCTCGAGCTCTACGAGGCTTATGCCACGTACCACGAGATCATGGATCTGA
CCGAGCAGGTGATCCGCGACACCGCCCAGGGCGTACTCGGCACCACCCAGGTCAGCTGGGACGGCGCCGACATTGATTTG
GCGCCGGCTTTCCGACGCTGGCGCATGGATGAGGCGGTGCGCCACCACAACCCGGAGATCAGCGCGGCCGATTGCACCGA
CCGCGCGGCGCTGCTGCGCCATTGCGAGCGGCTCAAGATCAAGGTCAAGCCGTCCTACGGCTGGGGCAAGCTGCTGCTGG
AAATCTTCGAGGCCACCGTCGAACACACGCTGGTGCAGCCGACTTTCATCACCGACCATCCGGTCGAGGTGTCGCCGCTG
GCACGCGCCAGCGACACCGAGCCGGGGTATACCGACCGCTTCGAGCTCTTCATCAATGGCAAGGAGCTGGCCAACGGGTT
CTCGGAGCTCAACGACCCGGAAGACCAGGCGGCGCGTTTCCAGGCCCAGGTGCAGGCGAAGGACGGCGGCGACGACGAAG
CCATGCATTACGACGCCGATTACATCCGTGCCCTGGAGTACGGAATGGCGCCGACCGGCGGGCTGGGCATCGGTATCGAT
CGGCTGGTGATGCTGCTGACCGGCAGTACCTCCATTCGTGACGTGCTGCTGTTCCCGTACATGCGCCCGGAAGCCTGA

Protein sequence :
MTEQTPTPQLPADENSLIAERRAKLGALRGQGIAYPNDFVRTHFAGDLQAEFAEAETWTAEALEAADRSVKLAGRLMAKR
VMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGDIVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGL
SDVEQRYRQRYVDLIVTPEAREVFIKRSKIIRAIRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAP
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHEIMDLTEQVIRDTAQGVLGTTQVSWDGADIDL
APAFRRWRMDEAVRHHNPEISAADCTDRAALLRHCERLKIKVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPL
ARASDTEPGYTDRFELFINGKELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHYDADYIRALEYGMAPTGGLGIGID
RLVMLLTGSTSIRDVLLFPYMRPEA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 6e-122 53
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 9e-75 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001903545.1 lysyl-tRNA synthetase VFG1668 Protein 4e-122 53