Gene Information

Name : fliI (CLL_A0843)
Accession : YP_001885044.1
Strain : Clostridium botulinum Eklund 17B
Genome accession: NC_010674
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 861757 - 863064 bp
Length : 1308 bp
Strand : +
Note : identified by match to protein family HMM PF00006; match to protein family HMM PF02874; match to protein family HMM TIGR01026; match to protein family HMM TIGR03496; match to protein family HMM TIGR03497

DNA sequence :
ATGATAGATATTGATTTTAATAACTTAATCAAGAAGGTTAATAATACTAGTTCAATATATACTGAAGGCGTGGTTAATAA
AGTAATTGGTCTTACAGTTGAAGTAAAAGGCATAAAGGCATTCGTAGGAGAATTATGCATTATATATAATGAAAAAAATG
CTCCTGTTAATTGTGAAGTCGTTGGATTTAAAGATGGTTTTGTTGTTTTAATGCCTTTAGATGAATTAATAGGAATTTCT
CCAGGATGTAGAGTAGTTCCAATGCGTAAGCCTTTAAGCGTAAAATGTTCTGATAAATTGTTAGGTGAAATTATTGATGG
ACTAGGAAAGCCGCTAAGAGGTGAAGAACTTGTTCAAGGAGAAGACTACCCATTAGAAAATAATCCACCAGACCCTTTGA
AAAGAAAAAGAATTAAAGAAATAATGCCAACAGGTATTAGAGCTATAGATGGATTTTTAACTTGTGGAGATGGTCAAAGA
ATAGGTATTTTTGCAGGTAGTGGAGTTGGTAAGAGTACTACTCTTGGAATGATAGCAAGAGAAGCAAAGGCAGATGTTAA
TGTTATTGCTTTAATTGGAGAAAGAGGTAGAGAGGTTCTGGAATTTATAGAAAAAGATTTAGGACCAGAAGGTATGAAAA
AATCAGTTGTAGTTTGTGCGACTTCTGATAAACCAGCTTTAATAAGATTAAAAGGTGCTCTTACAGCCACTGCAATAGCA
GAATATTTTAGAGATAAAGGTAAAAAAGTAATTTTAATGATGGATTCTGTTACAAGATTTGCGATGGCTCAGAGAGAAGT
AGGTCTTGCAATAGGAGAGCCTCCTGCAACAAAAGGATATACCCCATCGGTATTTGCTAAGTTACCAAAATTAATGGAGA
AATCTGGAACATCAGAAGATGGATCAATAACTGCTTTTTACACAGTTTTGGTTGATGGAGATGATTTTAATGAACCAATA
GCAGATGCTGTAAGAGGTATATTAGATGGTCACATAGTATTATCAAGATCTCTTGCTCATAAAAATCATTACCCTGCAAT
TGATATATTAAATAGTGTAAGTAGACTTATGAATTCAATAGCTCCATCTGAGCATATAAAAGCAGCTTCTATTGCTAGAG
ATCTTTTAGCAACATATAAAGAATCAGAGGATTTAATTAATATAGGAGCATATGTAAAAGGTAGTAATAAAAAAATAGAT
TTAGCAATTTCTTATCATGATAAAATAGAAGAGTTTCTGCGTCAGACTGTTAATGAAAAATCAAATTTTGATGAGAGTAT
AAGTTATTTAGTATCAATGTTTGAATAG

Protein sequence :
MIDIDFNNLIKKVNNTSSIYTEGVVNKVIGLTVEVKGIKAFVGELCIIYNEKNAPVNCEVVGFKDGFVVLMPLDELIGIS
PGCRVVPMRKPLSVKCSDKLLGEIIDGLGKPLRGEELVQGEDYPLENNPPDPLKRKRIKEIMPTGIRAIDGFLTCGDGQR
IGIFAGSGVGKSTTLGMIAREAKADVNVIALIGERGREVLEFIEKDLGPEGMKKSVVVCATSDKPALIRLKGALTATAIA
EYFRDKGKKVILMMDSVTRFAMAQREVGLAIGEPPATKGYTPSVFAKLPKLMEKSGTSEDGSITAFYTVLVDGDDFNEPI
ADAVRGILDGHIVLSRSLAHKNHYPAIDILNSVSRLMNSIAPSEHIKAASIARDLLATYKESEDLINIGAYVKGSNKKID
LAISYHDKIEEFLRQTVNEKSNFDESISYLVSMFE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-100 54
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-101 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-101 51
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-100 51
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-100 51
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-101 51
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-100 51
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-101 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-99 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-99 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 8e-86 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 5e-84 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-83 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-83 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-83 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-84 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-88 49
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-88 49
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-83 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-83 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-83 48
escN AAK26715.1 EscN Virulence LEE Protein 3e-83 48
escN AAL57542.1 EscN Virulence LEE Protein 3e-83 48
escN AAC31513.1 L0034 Virulence LEE Protein 2e-83 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-83 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-83 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-83 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-83 48
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-83 48
escN AAC38383.1 EscN Virulence LEE Protein 1e-83 48
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-81 47
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 5e-81 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-73 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 5e-76 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-73 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-79 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-79 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-79 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 8e-75 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 6e-76 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-68 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 3e-68 42
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-84 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-68 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-69 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-69 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-69 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-69 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-71 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-71 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG2144 Protein 2e-112 53
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0390 Protein 2e-99 53
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG1254 Protein 2e-100 52
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0048 Protein 3e-98 52
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0192 Protein 2e-98 51
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0515 Protein 3e-84 50
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0812 Protein 1e-83 48
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0730 Protein 6e-84 48
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG2499 Protein 4e-89 48
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG2331 Protein 3e-88 47
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG2459 Protein 6e-70 42
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG1778 Protein 1e-70 42
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG0555 Protein 1e-69 41
fliI YP_001885044.1 flagellar protein export ATPase FliI VFG1008 Protein 4e-61 41