Gene Information

Name : pyk (CLL_A0557)
Accession : YP_001884761.1
Strain : Clostridium botulinum Eklund 17B
Genome accession: NC_010674
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 556394 - 557812 bp
Length : 1419 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAAACTAAAATGATTTTTACTATTGGACCTGCAAGTGATAATGAGGAGATCATAAGGAAATTTATAGGAATTGG
TATGAATGCGGCAAGATTAAATTTTTCGCATGGTACTTATGAATCACACAGAGAGAAGATAGAATTAATAAAAAAAGTAA
GAGAAGATATGGGAACTCCGACTGCGATAGTCTTAGACATAAAAGGACCTAAGATAAGAACACATAACTTTGTAAATGAT
GGGGTAGAATTAAAAACTGGTCAAGAATTCTGTTTCGTATGCGGAGATGAACTTCTTGGTGATGAAGAAAGATGTTCTAT
TACTTATGAAGAGCTTTATAAAGACATACAAGTGGGTGGAGAAATTCTTGTTGATGATGGTCTTTTAAGATTTAAAGTTA
TAGCTGTTGAAGGCAAATCTATTAGATGTGCTGTAACAGTTGGTGGAATGATTAAAAATCACAAAGGTGTTAACGTTCCC
AACGTAATAATAAGACTACCTTCAGTAACAGAAAAAGATATAGCTGATATAAAATTTGGTTGTGAAATGGGTGTAGACTT
TATAGCAGCATCTTTCATAAGAAAAGCTAGCGATGTAGCTGATGTAAAGAAGATACTTTCTGAAAATAATGGTAAACATA
TTAAAGTAATAGCTAAAATTGAAAATCAAGAAGGCGTAGATAACATAGATTCTATAATAGAACAAACTGACGCTGTAATG
GTTGCTAGAGGAGATATGGGAGTTGAAATTCCTATTCAAAAAGTTCCTATAATTCAAAAAATGATAATTAGAAAATGTAA
TGATTCAGGAAAATTCGTTGTAACAGCAACTCAAATGCTTGATTCAATGATTAGAAACTCTTTACCAACTAGAGCAGAGG
CAAGTGATATTTGCAATGCTATATTTGATGGAACTGATGCTATAATGTTAAGTGGTGAAAGTGCCTCTGGAAATTTCCCA
ATTGAGGCAGCTACAACAATGTCTGAGATTGCGCAAGAAGCAGAAGCTCATTTAGATTATGCTCACTTAACAGCAAGGTT
AAGAGAACCCTCTCTTACTGATTATTCTGGTGCAATAAGTTATACAGCTTGTAGAACTTCAAATTTATTAAATGCAGCTG
CAATAGTAGCTGCTACTAAAAGTGGTGCTACAGCTACTATGATTTCAAGATATAGACCAAAATCTCCTGTTATAGCAATA
ACTCCTTATGAACAAGTAAGAAGAAGTTTAGCTTTAACTTTTGGTGTATTACCAATGCTTTGCGAAATGTATAATACTAC
TGATGAAATCTTAGTGGAAGCTAGAAGATCAGTGTTTGAATTAAATTTTGCTTTACCTGGTGATGATATTGTAGTTGCTG
CTGGAATGCCAACAACTCAAACTGGTGGAACAAATATGTTAAAAATAGAGAAATTATAA

Protein sequence :
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKKVREDMGTPTAIVLDIKGPKIRTHNFVND
GVELKTGQEFCFVCGDELLGDEERCSITYEELYKDIQVGGEILVDDGLLRFKVIAVEGKSIRCAVTVGGMIKNHKGVNVP
NVIIRLPSVTEKDIADIKFGCEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
VARGDMGVEIPIQKVPIIQKMIIRKCNDSGKFVVTATQMLDSMIRNSLPTRAEASDICNAIFDGTDAIMLSGESASGNFP
IEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYSGAISYTACRTSNLLNAAAIVAATKSGATATMISRYRPKSPVIAI
TPYEQVRRSLALTFGVLPMLCEMYNTTDEILVEARRSVFELNFALPGDDIVVAAGMPTTQTGGTNMLKIEKL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-90 44
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-90 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-90 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-89 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_001884761.1 pyruvate kinase VFG0479 Protein 1e-90 44