Gene Information

Name : pyk (CLL_A3336)
Accession : YP_001887522.1
Strain : Clostridium botulinum Eklund 17B
Genome accession: NC_010674
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 3509864 - 3511279 bp
Length : 1416 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGAGAAAGACTAAAATGATTTGTACTATTGGACCAGCAAGTGAAGACAGAGAAATATTAGAACAAGTAATGTTAGCAGG
AATGAATGCTTCAAGACATAACTTTTCACATGGTGATCACGAAGAACATAGAGGAAGAATAGAAAAGGTTAAAGAATTAT
CAAAGAAACTTGATAAAGAAATAGCTATCATCCTTGATACAAAAGGACCAGAAATAAGAACTGGAAAATTCGAACCAAAC
AAAGTTGAATTAGTTAAAGGTACAGAATTTACTGTATACGCAGGAGATATGTCAGTAGTTGGAGACACTACAAAGTGTTC
AGTTACTTACGAAGGATTAGCTAATGATGTTAAAGCAGGAAACACAATCTTAATAGATGATGGTTTAGTAGGATTAACAG
TTAAATCAGTAAAAGGAAACGCAGTTATCTGTGAAGTTCAAAATACTGGATTAGTTGGAACTCATAAAGGCGTTAACGTA
CCAGGAGTTTCAATCCAATTACCAGCTCTTACAGACAAAGATAAATCAGATTTAATATTTGGTTGTGAAATGGGAGTTAC
TATGATAGCAGCTTCATTCGTAAGAAAAGCAGCTGACGTAGTAGCTATAAGAAAAGTTCTTGATGAAAATGGTGGAAAGA
ACATCTTAATCTGCCCTAAAATCGAAAACCAAGAAGGTGTTGATAACATCGATTCAATATTAGAAATCTCAGATGCTATA
ATGGTAGCTAGAGGAGATCTAGGTGTTGAAATTCCAATAGAACAAGTACCAGCAGTTCAAAAAATGATCATTCAAAAATG
TAATGCAGCTGGAAAGCCAGTTGTTACTGCAACTCAAATGTTAGATTCTATGATCAGAAACCCAAGACCAACAAGAGCAG
AAGTTTCAGATGTAGCTAACGCTATATTAGATGGTACTGATGCAATAATGTTATCAGGAGAAAGTGCTAACGGAACTTAC
CCAGTAGAAGCTGTTAGAACTATGGCTAAAATTGCTGAAGAAACTGAAAAGCAATTAGCTCATAAAGTTGCTTACTCAAA
TGATAAATCTTCAATATCTGAAGTAATTTCAAGAGCAGCATGTAATGCAGCTAATGAATTAGAAGCAACTGCTATAATTT
CTTCAACTCAAACAGGAGCTACTGCTAAGAGAATATCTAAGTCTAGACCAGATTGTACAATCATAGCTGTTACTCCAGAT
GAAGTAGTAGCAAAACAATTAGCATTCTCATGGGGAGTTTACCCAATAGTTGCTGATAAAATGTGTTCAACTGACGAAAT
GATGATAAAATCAGTTGAAATAGCAAAAGAACATAACTATGTTAAAAATGGTGATACAGTAGTTTTAGCTGCTGGAGTTC
CAGTTGATAAAACAGGAACAACTAACCTATTAAAAGTTAGCGTAGTAGGCGAATAA

Protein sequence :
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKEIAIILDTKGPEIRTGKFEPN
KVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAGNTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNV
PGVSIQLPALTDKDKSDLIFGCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVANAILDGTDAIMLSGESANGTY
PVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAACNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPD
EVVAKQLAFSWGVYPIVADKMCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 9e-110 54
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-110 54
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-110 54
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-109 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_001887522.1 pyruvate kinase VFG0479 Protein 1e-110 54