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Name : leuS (CLL_A3149) Accession : YP_001887335.1 Strain : Clostridium botulinum Eklund 17B Genome accession: NC_010674 Putative virulence/resistance : Unknown Product : leucyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG0495 EC number : 6.1.1.4 Position : 3262009 - 3264459 bp Length : 2451 bp Strand : + Note : leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl DNA sequence : ATGGAAAATTACAGTACTAAAATCGATGAAAAATGGCAAAAGATTTGGGAAGAAAATGAAACTTATAAATTCAACCCAGA AAATTTAGATAAGAAACTTTATACACTTGAAATGTTCTCTTATCCATCAGGAGCTCAATTACATGCTGGTCACTGGTTTA ACTACGGACCAACAGATTCATGGGCAAGACTTAAAAGAATGCAAGGTTATAATGTGTTCCAACCAATGGGTTTTGATGCT TTTGGATTACCTGCTGAAAACTATGCAATAAAAACAGGTATACATCCTAAAGATTCTACATTTAAAAATATTGAAACTAT GGAAAAGCAATTAAAATCTATGGGCGCTATGTTTAATTGGGAAAATGAAGTTGTCACTTGTTCTCCTGATTACTATAAAT GGACTCAATGGTTATTCTTAAAACTTTATGAAAAAGGATTAGCTTACAGAAAAAAAGCTCCCGTTAATTGGTGTCCATCT TGTAATACAGTTTTAGCTAATGAGCAAGTAGTTGATGGCTTATGTGAAAGATGTGAATCTGAAGTTATTAAGAAAGATCT AATTCAATGGTTCTTCAAAATAACTGATTATGCTGATGAATTACTAGATAAATTAGATGGATTAGATTGGCCAGAAAAAA CTAAATCTATGCAAAAGCATTGGGTTGGGAGATCATATGGTGCACAAGTTACATTTAAAGTTAAAGATTCTAGCTTAGAA TTTGATGTATTTACTACAAGAGTTGATACTCTAAATGGTGTAACTTATGTTGTATTAGCACCCGAAAATAAATTAGTTAA TGAATTAACTCTTCCACAATACAAGGAAGCTGTTGAAAAATACAAAGAAGAAGCTAGCAAGCAATCTGATATAGAAAGAC AATCATCATCAAGAGAAAAAAGTGGTGTATTTACTGGATCATATGCAATAAATCCTATAAACGGAAAAGAAGTTCCTGTA TGGATTGCTGATTATGTATTAGCTACATATGGTACTGGTTGTGTTATGGCAGTTCCAGCACATGACGAAAGAGATTTCGC ATTTGCAAGTAAATTTAATTTACCAATAGAAAGAGTTATTACTAATAAGCAAGGTGATGAACCAGAACTTCCATTCTGTG AACATGGAATACTTGTTAATTCAGGACAATTTGATGGTTTAACTACAGATGAAGCTAAAAAAGCTATTGTTAGTGAACTT GAAAAAGATGAATTAGGACAAATGAAAGTTAACTTCAGATTAAGAGATTGGTTAGTTTCAAGACAAAGATACTGGGGAGC TCCTATTCCAGTTATATACTGTGATGAATGTGGAATAGTTCCAGTTCCTGAAAAAGACTTACCAGTAGAACTTCCTTATA ATGTTGAGTTTACTCCAGATGGTAAATCACCACTTGGAAAATGTGAAGAATTTATAAATACTACTTGTCCTCACTGTGGT AAACCTGCAAAAAGAGAAGCAGATACTTTAGATACATTTGTATGTTCTTCTTGGTATTATTTAAGATACCCTGATAACAA AAATACTGATGCACCTTTTGATCCTGAATTAATAAATAAAATGTTACCTGTAGATAAATATGTTGGTGGACCAGAGCATG CTTGTATGCATTTATTATATGCAAGATTTATAACTAAAGCATTAAGAGATATGGGTTACTTAAAATTTGATGAACCATTC TTAAGCTTAACTCACCAAGGATTAATCTTAGGACCAGACGGATTAAAAATGAGTAAATCAAAAGGAAATACAATCTCTCC AGATGACTATATTAAAGAGTTTGGTGCTGATGTATTTAGAATGTACTTAATGTTCGGTTTCGCATATGTTGAAGGTGGCG CATGGAGTGATGATGGAGTTAAATCTGTTGCTAGATTTGTAGATAGAATTGAAAGAACTTTAGAATCATGCAGAGATATC ATTAATGCTTCAGAAAATATTAAGACTACTATTGATTCTGAAGAAAAAGACTTAAACTTCTGGAGACATACAGCTATTAA AGGTGTTAGTGAAGATGCAGAAAAAATGCAATTTAATACTGCAATAGCAAGACTTATGGAATTTACAAATGCTATTCATA AATATATTCAAGCTGATACTAAAAATCCTACATTCTTAAAAGAAACTGTAGTAGATTTCTTAAAATTATTAGCACCATTT GCCCCTCACTTTACTGAAGAACATTGGAACTTATTAGGTCATAACTCAACTATATTTAATGAAAAATGGCCTGAATTTAA TCCAGCTGCTTTAGTTAAAGATGAAGTTGAAATAGCTATTCAAGTTAATGGTAAAATAAAAGCTAAAATTATGATACCAT CAAACTTAGATGAAGAAGGTATAAAGAAATCTTCATTAGAAAATGAAACTATAAAAGCAAATACTGAAGGTAAAAATATC ATAAAAGTAATTGTTATAAAAGGTAGACTTGTTAATATAGTTGTAAAATAG Protein sequence : MENYSTKIDEKWQKIWEENETYKFNPENLDKKLYTLEMFSYPSGAQLHAGHWFNYGPTDSWARLKRMQGYNVFQPMGFDA FGLPAENYAIKTGIHPKDSTFKNIETMEKQLKSMGAMFNWENEVVTCSPDYYKWTQWLFLKLYEKGLAYRKKAPVNWCPS CNTVLANEQVVDGLCERCESEVIKKDLIQWFFKITDYADELLDKLDGLDWPEKTKSMQKHWVGRSYGAQVTFKVKDSSLE FDVFTTRVDTLNGVTYVVLAPENKLVNELTLPQYKEAVEKYKEEASKQSDIERQSSSREKSGVFTGSYAINPINGKEVPV WIADYVLATYGTGCVMAVPAHDERDFAFASKFNLPIERVITNKQGDEPELPFCEHGILVNSGQFDGLTTDEAKKAIVSEL EKDELGQMKVNFRLRDWLVSRQRYWGAPIPVIYCDECGIVPVPEKDLPVELPYNVEFTPDGKSPLGKCEEFINTTCPHCG KPAKREADTLDTFVCSSWYYLRYPDNKNTDAPFDPELINKMLPVDKYVGGPEHACMHLLYARFITKALRDMGYLKFDEPF LSLTHQGLILGPDGLKMSKSKGNTISPDDYIKEFGADVFRMYLMFGFAYVEGGAWSDDGVKSVARFVDRIERTLESCRDI INASENIKTTIDSEEKDLNFWRHTAIKGVSEDAEKMQFNTAIARLMEFTNAIHKYIQADTKNPTFLKETVVDFLKLLAPF APHFTEEHWNLLGHNSTIFNEKWPEFNPAALVKDEVEIAIQVNGKIKAKIMIPSNLDEEGIKKSSLENETIKANTEGKNI IKVIVIKGRLVNIVVK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| leuS | YP_281511.2 | leucyl-tRNA synthetase | Not tested | Not named | Protein | 2e-167 | 45 |
