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Name : lysS (CLL_A0178) Accession : YP_001884425.1 Strain : Clostridium botulinum Eklund 17B Genome accession: NC_010674 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 192951 - 194456 bp Length : 1506 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCAACTGAGGAAATGAATATAAGTGAATTAGAATCTCAGTATAATGAACAAGAAGCATTAAGAAGAACTAAATTAGC AGATTTACAAGAAGCTGGTAAGGATCCTTTTGATGTTTATAAAGTAGAGAGAACTCATACATCAGAAGAAGTTAAATCTA ATTATGAAGAATTAGAAGGAAAAAATGTAACAGTTGCAGGAAGAATAATGTCAAAGAGAGGACAAGGAAAAGTTGTCTTT TCAGATATTCATGATAGAGATGGAAAAATCCAATTATTTATAAAGATTGATCTTGTAGGAGAAGAAAGTTTAAAATCATA TAAAACTTATGATCTAGGAGATTGGGTTGTAGCAACAGGAGAAGTATTTACTACAAAAACAGGAGAAGTATCTGTTAAAG TATCTAACTTTGAATTGATATGTAAGTCTTTAAAACCATTACCAGAAAAATGGCATGGATTAAAAGACCCAGATTTAAGA TATAGACAAAGAGAAGTAGATATAATAACTAATCCAGAAGTAAAAGAAACTTTTATAAAAAGAAGTAAAATTATTTCAGG AATTAGAGAATTTTTAGATACTAAGGGTTTCTTAGAAGTAGAAACTCCAATACTTGCGCCAATTGCAGGAGGAGCAGCTG CTAAACCTTTCGTTACTCATCATAATACTTTAGATATAGATATGTACTTAAGAATTGCAACAGAATTATATCTTAAAAGA TTAGTTGTAGCTGGATTTGAAAAAGTATATGAAATAGGAAGAAACTTCAGAAATGAAGGAATGTCAGTAAGACATAATCC TGAATTTACTGCTATTGAATTATATGAAGCATATTCTGATTATAATGATATGATGGAAATAACAGAAAATATGGTTGCAT ATGTTTGTGAAAAAGTAAATGGAACTACAAAGGTTACTTATCAAGGTACAGAAATAGACTTTATGCCACCATGGAGAAGA GTAACTATGGTTGATGTTGTTAAAGAACATGCAGGTGTAGATTTTACTACAATAAAATCTGATGAAGAAGCTCAAGCTAT AGCAAAAGAAAAACATTTAGAATTTGCTAAGCCTTTAAATACAGTAACAAAAGGTGAAGTATTAAATGCTTTATATGAAG AATTTGGTGAAGCAAATATGATTCAACCTACATTTGTTATGGATTATCCAGTTGAAATTTCACCTCTTACTAAAAAGAAG AGAGGGAATGAAGAATTTACTGAAAGATTTGAAGGATTTGTATTTGGAAGAGAATTATGTAACGCTTATTCAGAATTAAA TGATCCTGTAGTTCAAAGACAAAGATTTGAACAACAAGCTAAGGAAAGAGAACTTGGTGATGACGAAGCATATATGTTAG ATGAACAATTTATGAGTGCGTTAGAAACAGGTATGCCACCAACAGGTGGAATGGGAATGGGTATAGATAGACTTATTATG TTCTTAACAGATGCATCATCTATAAGGGATGTAATATTATTCCCAACAATGAAACCACAAAAATAG Protein sequence : MSTEEMNISELESQYNEQEALRRTKLADLQEAGKDPFDVYKVERTHTSEEVKSNYEELEGKNVTVAGRIMSKRGQGKVVF SDIHDRDGKIQLFIKIDLVGEESLKSYKTYDLGDWVVATGEVFTTKTGEVSVKVSNFELICKSLKPLPEKWHGLKDPDLR YRQREVDIITNPEVKETFIKRSKIISGIREFLDTKGFLEVETPILAPIAGGAAAKPFVTHHNTLDIDMYLRIATELYLKR LVVAGFEKVYEIGRNFRNEGMSVRHNPEFTAIELYEAYSDYNDMMEITENMVAYVCEKVNGTTKVTYQGTEIDFMPPWRR VTMVDVVKEHAGVDFTTIKSDEEAQAIAKEKHLEFAKPLNTVTKGEVLNALYEEFGEANMIQPTFVMDYPVEISPLTKKK RGNEEFTERFEGFVFGRELCNAYSELNDPVVQRQRFEQQAKERELGDDEAYMLDEQFMSALETGMPPTGGMGMGIDRLIM FLTDASSIRDVILFPTMKPQK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 2e-89 | 46 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001884425.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 9e-90 | 46 |
