Gene Information

Name : lysS (Npun_R5202)
Accession : YP_001868477.1
Strain : Nostoc punctiforme PCC 73102
Genome accession: NC_010628
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 6441891 - 6443576 bp
Length : 1686 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCGGAAGAAGATATCCGAGCCGCAAGGCTGGAAAAAGTAGAACAACTCAAGCAGCTAGGGACTAATCCCTACGCCTA
TCGCTGGGAATCTACCCATCATGCAGCACAGTTGCAAGAACAGTTTGCCGATTTAGCCAGTGGTGAAGAAGTTGATTTAG
AAGTTGCGATCGCTGGACGCATTATGGCGCGTCGCGTTTTCGGTAAACTGGCTTTCTTCACCTTGCAAGATGAAACTGGC
ACAATTCAGCTTTATTTGGATAAAAATCGCATCCAAGAAAGCATGGCAGATATTGATGCTGATGCCTTCAATCATTTAAA
ACAACTCACAGATGCAGGCGATATCCTGGGAGTTAAAGGTACTATTAAACGGACTGAAAAGGGCGAGTTATCTGTCTACG
TTAAACAATATACTATCCTGACTAAATCCCTGTTGCCCCTACCCGACAAGTGGCATGGATTGACGGATGTTGCCAAGCGC
TACCGTCAGCGTTACGTTGACCTGATTGTTAACCCGGAAGTGCGGCAAACTTTCCGCCGTCGTGCCCAAATTACAGCTGG
GATTCGCCGTTATTTGGAACAGCGAGATTTTCTCGAAATTGAAACGCCAGTTTTACAGAGTGAAGCTGGAGGTGCAGATG
CGCGTCCATTTATCACCCACCACAACACCTTAGAAATGGAATTGTATCTGCGAATTGCCACAGAACTCCATCTCAAGCGG
TTGATTGTAGGTGGTTTTGAAAAGGTGTTTGAATTGGGAAGGATTTTCCGTAATGAAGGAATTTCGACTCGACACAATCC
CGAATTTACAACGATTGAAGTTTACCAAGCCTACGCCGACTACAACGATATGATGGCGCTGACTGAAGGGATTATTACCA
CTGTCGCCCAAGAGGTACTCGGCACGCTGCAAATTACCTACCAAGGGGAACCTATAGATTTAACACCACCTTGGCGGCGA
GTGACAATGCACGATTTAGTTAAAGAATTTACTGGCTTAGATTTCAATTCTTTCCAAACCTTGGAAGAAGCAAAAACAGC
AAGTAAAAATGCTGGTATTCCTGGTGTAGATGAAGCCAAATCAATTGGTAAATTACTGAATTTAGCCTTTGAAGAGAAAG
TAGAAGCTAATTTAATTCAACCTACCTTTGTAATTGATTACCCAGTAGAAATTTCGCCCCTAGCAAAACCCCACCGTTCT
CAACCTGGTTTGGTGGAAAGATTTGAGTTATTTATCGTCGGGCGAGAAACTGGGAATAGCTTCTCAGAACTTACAGATCC
CATCGATCAAAGAGAACGCCTAGAAGCCCAAGCCGAAAGAAAAGCTGCTGGCGACTTAGAAGCCCAAGGTGTAGATGAAG
ACTTTTTGACAGCCCTTGAATACGGTATGCCGCCTACAGGTGGCTTAGGGATTGGGATCGATCGCTTAGTAATGTTGTTA
ACTGATTCTGCCAGCATTCGGGATGTAATTGCTTTCCCTCTGCTTAAGCCTGAAGGCAGCGTTATTAAGCAATTTAGCTA
TGAACAAAAAACCCAAACACTGACTATTGAATTTGACAGTGGAAGTGTTTACGAGTATTTCAAAGTCCCTCCCAGTGTCA
AAGAAGACTTGGATAATGCACCGTCTAAAGGTCAACACTTTAACAAGTTCATTAAAGGAAAATTCAAGTTTGAACAGTTA
AGTTGA

Protein sequence :
MSEEDIRAARLEKVEQLKQLGTNPYAYRWESTHHAAQLQEQFADLASGEEVDLEVAIAGRIMARRVFGKLAFFTLQDETG
TIQLYLDKNRIQESMADIDADAFNHLKQLTDAGDILGVKGTIKRTEKGELSVYVKQYTILTKSLLPLPDKWHGLTDVAKR
YRQRYVDLIVNPEVRQTFRRRAQITAGIRRYLEQRDFLEIETPVLQSEAGGADARPFITHHNTLEMELYLRIATELHLKR
LIVGGFEKVFELGRIFRNEGISTRHNPEFTTIEVYQAYADYNDMMALTEGIITTVAQEVLGTLQITYQGEPIDLTPPWRR
VTMHDLVKEFTGLDFNSFQTLEEAKTASKNAGIPGVDEAKSIGKLLNLAFEEKVEANLIQPTFVIDYPVEISPLAKPHRS
QPGLVERFELFIVGRETGNSFSELTDPIDQRERLEAQAERKAAGDLEAQGVDEDFLTALEYGMPPTGGLGIGIDRLVMLL
TDSASIRDVIAFPLLKPEGSVIKQFSYEQKTQTLTIEFDSGSVYEYFKVPPSVKEDLDNAPSKGQHFNKFIKGKFKFEQL
S

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 8e-112 49
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-86 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001868477.1 lysyl-tRNA synthetase VFG1668 Protein 4e-112 49