Gene Information

Name : Npun_R0030 (Npun_R0030)
Accession : YP_001863775.1
Strain : Nostoc punctiforme PCC 73102
Genome accession: NC_010628
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 46657 - 48087 bp
Length : 1431 bp
Strand : -
Note : PFAM: pyruvate kinase; KEGG: ana:all2564 pyruvate kinase

DNA sequence :
ATGCGTCGAACCAAAATCATTTGTACTGTTGGGCCCGCTACATCTGCACCCGAAAATCTGCGAGGCTTGGTAGAAGCTGG
AATGAATGTGGCGCGGCTGAATTTTTCCCACGGCGCTTACGAATTCCATGCCCAAACTGCTCACTATCTCAGACAAATTA
GTAATGAGCAGCAAAAGCCGATCGCAATTATGCAAGACCTCTGTGGTCCCAAGATTCGTTTGGGAACTTTACCACCGGAA
GGGTTAAATTTAGAAGCTGGTACTGAAGTTACTTTTGTTTTGCAAGAAAAGGGTGAGAGTATTGACGAACTCCCCCTACC
ATTGCCGACTCTGTTCGCAATGGTGCGACCAGGTGAACCGATTTTAATTAATGATGGTCGCGTCAAGTTAATTGTTACCG
ATCGCGATGCCGATCGCATTCGCGCCCAAGTGAAAACTGGCGGGTTAATTTCCACACATAAAGGAGTAAATCTACCACAA
ACTCCTTTACCTGTTAGTTCCATCACCGAAAAAGACTTACTGGATCTACGCTTTGGGATTCAGTTGGGTGTAGATTGGGT
AGCGGTGTCCTTCGTGCAATCACCACAAGACTTAGAACCCGCCAAGCGCATGATTGAAGCGGCTGGTGCTTCCATCCGCT
TAATTGCCAAAATCGAAAGAGCAGAAGCACTAGAAAACTTTGATTCCATTCTGAAAGTTGCCGACGCAATTATGATTGCC
CGTGGCGATTTAGGGGTGGAAGTGCCAATTCACGAAGTACCCCTCATTCAAAAAGATATTATTCGCCGTTGCAATCGTGC
TGGCAAACCGGTGATTACAGCCACCCAAATGCTAGAGTCGATGATTAGCGCCCCCGATCCCACCCGCGCCGAAGCAACTG
ATGTTGCCAACTCCATCTTAGATGGTACGGATGCGGTAATGCTCTCTGGTGAAACAGCTGTCGGACAATATCCCATCGCC
GCCGTCCAGATGATGCACAACATCGCCGTGCGGACAGAACAGGCTCTAGATGAGGGTAGTAAACATGCTTGGTGTCATGA
AGCAGGCAGTCTTAGCGTTACCGAATCTGTAGCAGAATCTGTGTGTCGCATCGCTTATGAAACAGGTTCACGGGCAATTC
TCTGTAACACTTCATCAGGAAATACAGCGAGAATGGTGTCTAAATATCGGCCGACTTCTCCCATTATTGCCCTCACCTCC
GATATCACCGCTTATCGCCAACTAGCGCTTTCTTGGGGTGTGGAAGCTTTGCTGATCCCACCAGTCCACCATGCTGAAGA
GATGTTTACCAATGTGGTGAACACAGTTGTAGATATGGGTCTAGCCAATAAGGGCGATAAAGTAGTGATTACCTCTGGTG
TTCCAATTGGTAAATCGGGCACAACTAGTTTAATCAAAGTGCATTCCATTGGACAGCCAATATCCGCATAA

Protein sequence :
MRRTKIICTVGPATSAPENLRGLVEAGMNVARLNFSHGAYEFHAQTAHYLRQISNEQQKPIAIMQDLCGPKIRLGTLPPE
GLNLEAGTEVTFVLQEKGESIDELPLPLPTLFAMVRPGEPILINDGRVKLIVTDRDADRIRAQVKTGGLISTHKGVNLPQ
TPLPVSSITEKDLLDLRFGIQLGVDWVAVSFVQSPQDLEPAKRMIEAAGASIRLIAKIERAEALENFDSILKVADAIMIA
RGDLGVEVPIHEVPLIQKDIIRRCNRAGKPVITATQMLESMISAPDPTRAEATDVANSILDGTDAVMLSGETAVGQYPIA
AVQMMHNIAVRTEQALDEGSKHAWCHEAGSLSVTESVAESVCRIAYETGSRAILCNTSSGNTARMVSKYRPTSPIIALTS
DITAYRQLALSWGVEALLIPPVHHAEEMFTNVVNTVVDMGLANKGDKVVITSGVPIGKSGTTSLIKVHSIGQPISA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-80 42
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-80 42
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-80 42
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 8e-80 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Npun_R0030 YP_001863775.1 pyruvate kinase VFG0479 Protein 7e-81 42