Gene Information

Name : lysS (KRH_21510)
Accession : YP_001856004.1
Strain : Kocuria rhizophila DC2201
Genome accession: NC_010617
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 2474640 - 2477939 bp
Length : 3300 bp
Strand : +
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
ATGGACAAGACCCACGACCGCCTGCCACGGCTGGCCAGCGCCCTGGTGTTCCTGGCCTCCCTGTGGTCCCTCGTCTCCAT
CCCGTTCCCCCGCGGTGAGGCCCTCTACCTGGTGGACGACGCGTTCACCGCGCTCGGCGTCCCGGCCGGGCCCAACCTGT
TCCTCGCGCTGAGCCTGTTCATCGTGGGCGCCGCGCTGCGCCGCAGGCTGCGGATCGCGTGGTTCGTGTCCTTGGCCGTG
CTGGCGGTGGAGATGCTCGTGTACGTGCTCACGGGCGTCGTGCTGGTCTCGGACTCGCTGGAGGGTGAGCTGGACGCCCT
GGACTGGGTGCTGCTCAGCGTGGGCTGCGTGGTCACGCTCGCGTGGATCGTGGCGTTCGTCGTGCGCCGCCGCGACTTCC
CCGCACGGATGCGCCACGGCGCCACCCGCCAGGCCCTGCTCACCCTCGTGGCGGGCCTGGCCCTGGTGATCGTCCTGGTC
TCCACCGTCTCCTGGTTCATCCCCAGCCACCTGCACGGCATCGAGCACCTGTGGTTCTCCTTCCGCAGCGTCACGGGGCT
GTCCCTGCCGCGCAGCATCTCCGACGGCTCGGCGGGACCGCACTGGCTCGCGACGCTGGGCGGCGTGCTGGGTGCCGCCG
TGCTGTTCTACGCGGTGTGGAGCTTCACGCAGTCCGCGCAGCGCTCCGAGGTGATGGGCCCGGAGGACGAGCTGCACGTG
CGCCGGCTGCTCGCCACGAACGGCAACCAGGACTCCCTGGGGTACTTCGCCACCCGTCGGGACAAGTCCGTGATCTTCTC
CGCGGACGGGCGTGCCGCCGTGTCCTACCGCGTGATCGGCTCCGTGTGCCTGGCCTCCGGGGACCCCCTGGGCCACCCGG
ACGCGTGGCAGGACGCCATTGCCGCGTGGCAGCGCGAGGGCCGCGTGCACGCGTGGCGCATGGGCGTGCTCTCCGCCTCG
GAGACCGGCGCCCGCGCGTACATCAAGGCCGGCCTGAAGGCCCGCCCGCTCGGGGACGAGGCCGTGCTCGACACCGAGTC
CTTCACCCTGGAGGGCCGGGCCATGCGCCCGGTCAAGCGCGCGGTCGCCCGCGTGTGCGAGGCCGGTTGCACCGTCACCG
CCGAGCGCCACAGCCAGCTGGACGCCGCCACGATGCAGCAGATCATCGACCTCTCCGAGCAGTGGCGCGGGGACGAGGCG
GACCGCGGCTTCTCCATGGCCCTTAACCGCCTCGCGGACCGCACGGACGGGCGAAGCGTGGCGGTGCTCGTGCGGGACGC
CTCCGGGCAGATCGTGGCCCTGCAGTCCTACGTGCCGTGGGGCACCCGCGGCCTGTCCCTGGACCTGATGCGCCGCTCGC
CGCAGGCCCCCAACGGCGTCAACGAGGCCATGGTGGCCACCCTCGCCGAGGAGGGCGGGGATCTTGGCGTGCGGGAGATC
TCCCTGAACTTCGCGATGTTCCGCTCCGTGTTCACCGGCGCGGACAAGGTGGGCGCGGGCATCGGCGTGCGCCTCGCGGA
CCGCGTGCTCACGTGGGGCAACCGCTTCTGGCAGCTCGAGTCGCTCTACGAGGCCAACGCCAAGTACCTGCCCCGCTGGG
ATGCGCGCTTCGTCTGCTACGACTCCACTGCCAACCTGCCCTACATCGGCATCGCCGCGGGCCGCGCCGAGGGCTTCCTC
CCGGGCCGTCCGCCGCGCCCCGGTCACGACGGCGAGGCCCTGCTGGCCGGGCCCGACGGCGCTCCCACCCCCCTCGCGGA
CCTCGCCATCGCGCAGGACCGCGAGCTGCTCACCCCGCCCCGCCCGGTGCGCCACCTCACCGAGCAGCAGCGGGTCCGCC
ACGCCAAGCTGGATGTGCTGCGCGAGGCCGGGATGGAGCCCTACCCCGTGGGTGTGCCCTACACGATGTCCCTGGAACGG
GCACGGCAGATCATCGAGGAGGGCGCGGAGGCCACTGTCGCCGAGCCCGTCTCGCTGGGAGGGCGCGTGCGCGAGCTGCG
TGACTTCGGCGGCGTCGTCTTCGCCGTGATCGAGGAGCACGACGTGCGGCTGCAGGTGATCCTGGAGGACTCCGCCGTGA
GCCCCGAGCTGCTGAGCCTGTGGCGGCGCGCGGTGGACGTGGGGGACCACATCTCCGCGACCGGCACGCTTGGCCGCAGC
CGCCGCGGGGAGCCCTCCCTGCTGGCGACCTCCTGGGCCATGGCCTCCAAGTCCCTGCGCCCCGTGCCGAGCGACCACAC
CGGGTTCACCAACCCGGAGGCGCGCGTGCGCCAGCGCTACCTGGACCTGATCGTGAACAAGGACTCCGCACGGCTGCTGC
GCGCCCGCTCGCGCGGCATCGCGGCCATGCGGCAGTCCTTCATGGAGCGCGGCTACATGGAGGTGGAGACCCCCATGCTG
CAGGCCGTGCACGGCGGAGCAAGCGCCCGCCCGTTCCGCACCCACATCAACGCCTACAACGCGGACCTCTACCTGCGCAT
CGCCCCGGAGCTGTACCTCAAGCACCTGTGCGTGGGTGGCATGGACAAGATCTTCGAGCTCAACCGCAACTTCCGCAACG
AGGGCGCGGACGCCACCCACAACCCCGAGTTCACCTCCGTGGAGGCGTACGCGGCGCACGGCGACTACAACACCATGCGG
GTGCTCACCCGGGACCTCATCATCGAGGTGGCCACCGCGATCCACGGCGAGCCCGTGGCCGTGCGCCCGGACGGCAACGG
CGGGGAGACCCGCATCCGGCTGGACCACGAGTGGCCCGTGATCCCCGTACACCAGGCGGTCTCCGAGGCCACCGGCACCC
TGCTGACCTCGCACACCCCGCTGGAGGAGGTGCGGGCGGTGGCCTCAGCGCACAGCGTGCACTGGAGCGAGGAGATGACC
GCCGGTGAAGTGGTCCTGGAGCTCTACGACGAGCTCGTGGAGGGCCAGACCACCCTGCCGGTGTTCTACACGGACTTCCC
GCTGGAGACCTCCCCGCTGACGCGCACGCACCGCACGGACCCGAAGCTCTCGGAGCGCTGGGACCTGGTGGCGTTCGGCG
CGGAGATCGGCACCGCGTACTCGGAGCTCGTGGACCCCGTGGACCAGCGCAACCGCCTGACGGAGCAGTCCTACAAGGCC
GCCGCCGGCGATCCGGAGGCCATGGAGGTGGACGAGAACTTCCTCACCGCCCTGGAGTACGCCATGCCTCCCACGGGTGG
CCTGGGCATCGGCGTGGACCGGATGATCATGATGCTCACGGGCACCAACATCCGCGCCACCCTGGCGTTCCCGTTCACCC
GCCCCAACGAGCAGCGCTGA

Protein sequence :
MDKTHDRLPRLASALVFLASLWSLVSIPFPRGEALYLVDDAFTALGVPAGPNLFLALSLFIVGAALRRRLRIAWFVSLAV
LAVEMLVYVLTGVVLVSDSLEGELDALDWVLLSVGCVVTLAWIVAFVVRRRDFPARMRHGATRQALLTLVAGLALVIVLV
STVSWFIPSHLHGIEHLWFSFRSVTGLSLPRSISDGSAGPHWLATLGGVLGAAVLFYAVWSFTQSAQRSEVMGPEDELHV
RRLLATNGNQDSLGYFATRRDKSVIFSADGRAAVSYRVIGSVCLASGDPLGHPDAWQDAIAAWQREGRVHAWRMGVLSAS
ETGARAYIKAGLKARPLGDEAVLDTESFTLEGRAMRPVKRAVARVCEAGCTVTAERHSQLDAATMQQIIDLSEQWRGDEA
DRGFSMALNRLADRTDGRSVAVLVRDASGQIVALQSYVPWGTRGLSLDLMRRSPQAPNGVNEAMVATLAEEGGDLGVREI
SLNFAMFRSVFTGADKVGAGIGVRLADRVLTWGNRFWQLESLYEANAKYLPRWDARFVCYDSTANLPYIGIAAGRAEGFL
PGRPPRPGHDGEALLAGPDGAPTPLADLAIAQDRELLTPPRPVRHLTEQQRVRHAKLDVLREAGMEPYPVGVPYTMSLER
ARQIIEEGAEATVAEPVSLGGRVRELRDFGGVVFAVIEEHDVRLQVILEDSAVSPELLSLWRRAVDVGDHISATGTLGRS
RRGEPSLLATSWAMASKSLRPVPSDHTGFTNPEARVRQRYLDLIVNKDSARLLRARSRGIAAMRQSFMERGYMEVETPML
QAVHGGASARPFRTHINAYNADLYLRIAPELYLKHLCVGGMDKIFELNRNFRNEGADATHNPEFTSVEAYAAHGDYNTMR
VLTRDLIIEVATAIHGEPVAVRPDGNGGETRIRLDHEWPVIPVHQAVSEATGTLLTSHTPLEEVRAVASAHSVHWSEEMT
AGEVVLELYDELVEGQTTLPVFYTDFPLETSPLTRTHRTDPKLSERWDLVAFGAEIGTAYSELVDPVDQRNRLTEQSYKA
AAGDPEAMEVDENFLTALEYAMPPTGGLGIGVDRMIMMLTGTNIRATLAFPFTRPNEQR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 0.0 46
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-180 46
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 0.0 46
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 0.0 46