Gene Information

Name : entE (ACICU_02578)
Accession : YP_001847237.1
Strain : Acinetobacter baumannii ACICU
Genome accession: NC_010611
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2731587 - 2733215 bp
Length : 1629 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGAAAAAACAGTTGATTGAGTTTGTTCGCTGGTCTCCCGAGAGAGCGCAACACTATCGAAATAAAGGCTATTGGATTGA
CCAACCTTTGACGCGCATTTTAACGGTAGGGGTTCAATCGCATCCGCATTCGCCGGCAATTATTTGTGGTGAACGTCAGT
TGAGTTATATCGAGCTGGATCGGCTATCAACAAATTTAGCTTCTCGACTAGCTGAAAAAGGCTTGGGCAAGGGTGATACA
GCTTTAGTACAACTTCCCAATGTTGCGGAGTTTTATATTGTCTTTTTTGCCTTGCTTAAAGCAGGCGTGGTTGTACTCAA
TGCGCTATATAGCCATCGCCAATATGAACTAAATGCCTTTATTAAACAGATTCAACCTAAGCTTTTAATTGGTTCGCGTC
AGCATGAAGTATTTAGCAATAATCAATTTATTGATTCACTTCATGAAGTAAATTTAAGTCCTGAAATTATTTTGATGCTC
AATCATCAAGCTACCGATTTCGGATTATTAGACTGGATTGAAACACCAGCAGAGACCTTAGTCGATTTTTCATCTACACC
TGCTGATGAAGTTGCTTTCTTTCAGCTATCAGGTGGAAGTACGGGAACACCAAAACTTATTCCACGCACGCATAATGACT
ATGACTATAGCGTGCGAGCCAGTGCCGAGATTTGCGATTTAAACTCAAATACCCGGTTATTGTGCGCTCTGCCAGCTCCG
CATAACTTTATGTTGAGTTCACCTGGTGCATTAGGTGTTTTACATGCAGGTGGATGTGTTGTTATGGCACCAAATCCTGA
ACCGCTCAATTGCTTCTCTATTATTCAAAGACATCAAGTCAATATGGCATCTTTAGTGCCGAGTGCTGTCATTATGTGGC
TAGAAAAAGCCGCGCAATATAAAGATCAAATTCAGTCTTTAAAGTTGCTTCAAGTAGGTGGAGCAAGTTTCCCTGAATCT
TTAGCCCGTCAAGTTCCTGAAGTCATCAATTGTAAATTACAACAAGTATTTGGAATGGCCGAAGGCTTGGTCAATTACAC
CAGACTCGATGACTCCGATGAGCAAATATTTACGACACAAGGGCGTCCTATCAGTTCTGATGATGAAATCAAAATTGTGG
ATGAACAGTATAAAGAGGTTCCAGAAGGTGAAATAGGGATGCTTGCGACTCGGGGACCTTATACCTTTTGCGGTTATTAC
CAAAGCCCTGAACATAATTCACAGGTCTTTGATGAGGACAACTATTACTATTCGGGCGATCTCGTGCAGCGTACCCCTGA
TGGTAATTTACGTGTAGTAGGAAGAATTAAAGACCAGATTAACCGTGGTGGCGAAAAGATTGCTTCGGAAGAAATAGAAA
AACTTATTCTTTTGCATCCAGAAGTTATGCACGCAGCTTTGGTCGCAATTGTTGATGAACAATTTGGTGAAAAGAGTTGT
GCCTTTATTGTTTCTCGTAATCCTGAACTTAAAGCTGTTGTGCTCAGACGCCATCTCATGGAGTTGGGTATTGCACAATA
CAAACTTCCAGATCAGATCAAATTAATCGAAAGTTTGCCACTGACCGCAGTCGGTAAGGTGGACAAAAAACAACTTCGCA
GCATTTTAAATACATCTACAACATCTTAA

Protein sequence : 
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELDRLSTNLASRLAEKGLGKGDT
ALVQLPNVAEFYIVFFALLKAGVVVLNALYSHRQYELNAFIKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILML
NHQATDFGLLDWIETPAETLVDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICDLNSNTRLLCALPAP
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQYKDQIQSLKLLQVGGASFPES
LARQVPEVINCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQGRPISSDDEIKIVDEQYKEVPEGEIGMLATRGPYTFCGYY
QSPEHNSQVFDEDNYYYSGDLVQRTPDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIESLPLTAVGKVDKKQLRSILNTSTTS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-82 41
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 41
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 41
ybtE CAA21394.1 - Virulence HPI Protein 5e-82 41
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 4e-82 41
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-81 41
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-82 41
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-81 41
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001847237.1 enterobactin synthase subunit E VFG0932 Protein 5e-142 56
entE YP_001847237.1 enterobactin synthase subunit E VFG0168 Protein 4e-105 43