Gene Information

Name : aphE (SGR_249)
Accession : YP_001821761.1
Strain : Streptomyces griseus NBRC 13350
Genome accession: NC_010572
Putative virulence/resistance : Resistance
Product : streptomycin 6-phosphotransferase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG3231
EC number : -
Position : 267117 - 267935 bp
Length : 819 bp
Strand : +
Note : -

DNA sequence :
ATGAGTGATCACCCCGGGCCGGGGGCCGTCACGCCGGAGCTGTTCGGCGTGGGCGGCGACTGGCTGGCCGTCACCGCGGG
CGAATCGGGCGCCTCCGTCTTTCGCGCCGCGGACGCCACCCGGTACGCCAAGTGCGTGCCCGCCGCGGACGCGGCCGGTC
TTGAGGCGGAACGCGACCGGATCGCCTGGCTGAGCGGGCAGGGCGTACCGGGCCCCCGCGTCCTCGACTGGTACGCCGGT
GACGCGGGCGCCTGCCTGGTCACCCGTGCCGTCCCCGGCGTACCCGCTGATCGGGTGGGCGCCGATGACCTTCGCACTGC
CTGGGGGGCCGTCGCGGACGCGGTCCGTCGGCTGCACGAGGTACCCGTGGCCTCGTGTCCGTTCCGCCGGGGGCTGGACT
CCGTGGTCGACGCCGCCCGTGACGTGGTGGCCCGTGGCGCGGTGCATCCGGAGTTCCTGCCGGTGGAGCAGCGGCTCGCT
CCCCCGGCGGAGCTGCTGGCCCGGCTCACCGGGGAGCTCGCCCGTCGGCGCGATCAGGAGGCCGCCGACACGGTCGTCTG
CCACGGTGATCTCTGCCTGCCCAACATCGTCCTCCATCCGGAGACCCTGGAGGTGTCGGGCTTCATCGACCTGGGACGGC
TCGGGGCGGCCGACCGCCACGCCGACCTGGCGCTGCTGCTGGCCAACGCGCGCGAGACCTGGGTGGACGAGGAGCGGGCG
CGGTTCGCCGACGCGGCGTTCGCCGAGCGCTACGGCATCGCCCCGGACCCGGAACGGCTGCGCTTCTACCTCCATCTCGA
TCCGCTCACCTGGGGCTAG

Protein sequence :
MSDHPGPGAVTPELFGVGGDWLAVTAGESGASVFRAADATRYAKCVPAADAAGLEAERDRIAWLSGQGVPGPRVLDWYAG
DAGACLVTRAVPGVPADRVGADDLRTAWGAVADAVRRLHEVPVASCPFRRGLDSVVDAARDVVARGAVHPEFLPVEQRLA
PPAELLARLTGELARRRDQEAADTVVCHGDLCLPNIVLHPETLEVSGFIDLGRLGAADRHADLALLLANARETWVDEERA
RFADAAFAERYGIAPDPERLRFYLHLDPLTWG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
strA CAJ77025.1 Streptomycin phosphotransferase A Not tested AbaR1 Protein 1e-40 51
strA YP_005176180.1 aminoglycoside 3''-phosphotransferase protein Not tested ICEPmu1 Protein 1e-38 50
strA ACK44539.1 StrA Not tested SGI1 Protein 9e-41 50
strA AEA34675.1 aminoglycoside phosphotransferase Not tested Not named Protein 9e-41 50
strA YP_001800932.1 aminoglycoside-3''-phosphotransferase Not tested Not named Protein 1e-40 50
strA AFH57207.1 streptomycin resistance protein Not tested AbaR4a Protein 9e-41 50
strA AEQ20909.1 StrA, APH(3')-Ib streptomycin(3') phosphotransferase Not tested Tn6166 Protein 1e-40 50
strA AEZ06049.1 StrA, APH(3')-Ib, streptomycin(3') phosphotransferase Not tested Tn6167 Protein 1e-40 50
strA AGK07031.1 StrA streptomycin phosphotransferase Not tested SGI1 Protein 9e-41 50
strA AGK07089.1 StrA streptomycin phosphotransferase Not tested SGI1 Protein 9e-41 50
strA AFV47972.1 streptomycin (3') phosphotransferase StrA Not tested AbaR25 Protein 9e-41 50
strA AFV48000.1 streptomycin (3') phosphotransferase StrA Not tested delta-AbaR25 Protein 9e-41 50
ABK1_0266 YP_005512838.1 StrA Not tested AbaR4d Protein 1e-40 50
BJAB07104_00282 YP_008207747.1 Aminoglycoside phosphotransferase Not tested AbaR25 Protein 1e-40 50
ABZJ_00266 YP_005524222.1 hypothetical protein Not tested AbaR22 Protein 1e-40 50
BJAB0868_00286 YP_008211612.1 Aminoglycoside phosphotransferase Not tested AbaR26 Protein 5e-40 50
ABK1_0258 YP_005512830.1 StrA Not tested AbaR4d Protein 2e-40 50

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
aphE YP_001821761.1 streptomycin 6-phosphotransferase DQ336355.1.orf1.gene Protein 1e-42 58
aphE YP_001821761.1 streptomycin 6-phosphotransferase AF024602.1.gene5.p01 Protein 6e-41 50
aphE YP_001821761.1 streptomycin 6-phosphotransferase K00432.1.orf2.gene.p Protein 2e-15 42