Gene Information

Name : lysS (Pnec_0485)
Accession : YP_001797365.1
Strain : Polynucleobacter necessarius STIR1
Genome accession: NC_010531
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 429109 - 430653 bp
Length : 1545 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAACGACAAATCGAATTTACCTCCAGTTACCGAAGCAATTGATGAGAATCACATCATTGCGGAGCGCCGTGAAAAGCT
AGCCAAGTTACGCAAAGGTGGGGTTGCATTTCCCAATGACTTTGCCCCTACACACTTGGCGGCAGATCTGCATTCGTACT
ATGACAGCCTCACTAAAAAAGAATTGGCTGCGAAGAAAATTCATGTCAAGGTTGCTGGACGCATGGTTCTTAAACGTGTG
ATGGGTAAAACAAGTTTCGCAACCATTCAAGATCGCAGCGGTCAGATTCAGTTCTACATCAATGATGAGTTGAGTGGCGC
CGATGTCCATGGCGCCTTCAAACATTGGGATATGGGTGACTTTATCTCCACTGAAGGCAATCTCTTTAAAACCAATAAAG
GCGAGCTATCAGTGGAGTGCAGCAATTTACGCCTCCTCAGCAAATCATTGCGTCCATTGCCAGATAAGTTCCACGGTCTC
TCAGATCTAGAGACTAAATATCGCCAACGCTATGTAGACTTGATTGTTAATCCAGAAAGTCGTAGTACATTTAAAGCGCG
CAGTAACGCTATCGCTTCTTTACGTCGCCATATGCTAGATGCCGACTTTATGGAAGTAGAGACCCCTATGCTCCACCCTA
TTCCCGGTGGTGCAGCAGCAAAGCCTTTTATTACCCACCATAATGCACTCGATATGCAAATGTTTTTGCGTATTGCACCT
GAGCTTTATCTCAAACGTTTAGTGGTTGGTGGCTTTGAACGTGTTTTTGAAATTAACCACAACTTCCGTAACGAAGGCGT
AAGTCCACGTCACAACCCAGAATTCACCATGATGGAATTCTATGCAGCTTACACAGATTACCGCTGGCTCATGGACTTCA
CTGAAGGATTAATTCGCGCTGCCGCAATTGATGCACAAGGCACTGCTGTATTGACCCATCAAGGTCGTGAGCTTGATCTG
AGCAAGCCGTTCCAACGCCTCACCATTACTGAAGCCATCCTGAAGTACTGCGGTCAATCAGGCAAGAGCTATGAAGCAGC
CCAGCTTGAAGACGCCAACTTCATTCGCGTTGAATTGAAAAAAGGTGGTGAGAATCCAGATGCTCCTACGCTCAAGAATG
CCGGCATTGGCGCACTCCAGCTAGCCCTCTTTGAATTGGTTGCAGAAGAGCACCTCTGGGAGCCAACCTACATCATTGAT
TACCCAATTGAAGTAAGCCCACTCGCCAGAGAATCGGATACTCGTCCAGGCATTACTGAGCGCTTTGAGCTGTTTATTAC
CGGTCGCGAAATTGCCAATGGCTTCTCCGAATTAAATGACGCTGAAGATCAAGCCAACCGCTTCCGCAAGCAAGTTGAGC
AAAAAGAAGCGGGCGACGAAGAGGCTATGTATTTTGATCATGACTTCATTCGCGCCCTCGAGTACGGCATGCCTCCAACT
GGCGGTTGCGGTATTGGTATCGATCGTTTAATAATGCTACTTACCGATGCTCCAAACATTCGTGATGTGATCCTCTTTCC
GCATTTGCGTCGCGAAGAAGATTAA

Protein sequence :
MNDKSNLPPVTEAIDENHIIAERREKLAKLRKGGVAFPNDFAPTHLAADLHSYYDSLTKKELAAKKIHVKVAGRMVLKRV
MGKTSFATIQDRSGQIQFYINDELSGADVHGAFKHWDMGDFISTEGNLFKTNKGELSVECSNLRLLSKSLRPLPDKFHGL
SDLETKYRQRYVDLIVNPESRSTFKARSNAIASLRRHMLDADFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFLRIAP
ELYLKRLVVGGFERVFEINHNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEGLIRAAAIDAQGTAVLTHQGRELDL
SKPFQRLTITEAILKYCGQSGKSYEAAQLEDANFIRVELKKGGENPDAPTLKNAGIGALQLALFELVAEEHLWEPTYIID
YPIEVSPLARESDTRPGITERFELFITGREIANGFSELNDAEDQANRFRKQVEQKEAGDEEAMYFDHDFIRALEYGMPPT
GGCGIGIDRLIMLLTDAPNIRDVILFPHLRREED

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-125 55

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001797365.1 lysyl-tRNA synthetase VFG1668 Protein 5e-125 55