Gene Information

Name : polA (CLK_2397)
Accession : YP_001788325.1
Strain : Clostridium botulinum Loch Maree
Genome accession: NC_010520
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3270570 - 3273197 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTACAAAGCAGGTAGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAGTAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA
TGGATTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAGGATGCTCTACAATTGGCTACGGATAACGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGATAGAGGAGTTTGGAGTTACACCTAAGGAATTTATAGATGTAAAAGGTCTTATGGGAGATAC
CTCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTACTTGAAAACATAGAGAATATAAAGGGGAAAAAATTAAAGGAAAATTTACATGAATATAGAGAGCAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAAAAATGTTTGAAAGGTTAGAATTTAAATCTTTAATGAATAAAGTAGGCAAAAGTGCTGAGGAAG
AAACTGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAAAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAATAAAAACATTATA
TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGTTGCTAAATAATACTAAAGAACAATTTATAGAAGTAT
GTAAAGAGATATTTGAAAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATACCTAATAGATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCAGAATATTTGTTTAAAAATATAAATAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTAGAAGAGAAAATAAAAGATCAAGATATGGAAGAATTGCTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCTGCTATGGAAACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGAGAAATAGAAAGTACTCAGTTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAATGCAGAGGTA
CTAGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGGA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATT
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAAGTAACGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCCGTTGAAAAAGGGTATGTAAGTACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGGGAAAGGCTAGCTATGAATAGTCCAATCCAAGGAAGTGCAGCA
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTTTATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAGAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGCTAGACGTGGATATAAATATAGGTAATAATTGGTATGAAGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI
YDKNRMIEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVRKMFERLEFKSLMNKVGKSAEEETVEEEVSYKNIVSLEEFKDLKNNIIK
YKENDLYLYFELEDIVLFSKSRIKTLYANFKDEVYRIDFEMLLNNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKDQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRSNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVYKDELEKIKALVEKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 2e-164 45