Gene Information

Name : pykA (CLD_1136)
Accession : YP_001783009.1
Strain : Clostridium botulinum Okra
Genome accession: NC_010516
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 3676188 - 3677945 bp
Length : 1758 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF00391; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAGACTAAAATGATATTTACAATAGGCCCTGCAAGCTCGACAGAAGAAATTGTATCAAAATTAATAGAAGCTGG
AATGAGTGTATCAAGACACAATTTTTCTCACGGATCACATCCAGAACACAAAGAAAGAATGATGATGATTAAGAAATTAA
GAGAAAAACATAACAAACACATAGCTATTATGTTAGACACCAAAGGACCAGAAATAAGAACAGGTAATTTTTCAGTGGAT
AAAGTGGAATTAAAAGAAGGGGCAGAGTTTACAATATATTGTAGAGAAGACATAGTAGGTGATGAAACAAAATGTTCTAT
TACTTATGATGAACTATATAAAGATGTAGTTAAAGGAAATAAAATACTGATAGATGATGGATTAGTAGAGCTAGAGGTTC
AGTCAGTAGAAGATAATAAAATCCATACAGTAGTTAAAAATTCAGGAACTGTTTCAAATCACAAGGGAGTAAATGTTCCA
GGAGTTTCAGTATCACTTCCAGCAGTTACTGAAAAAGATATAGAAGATTTAAAATTTGGATGTGAAGTAGGAGTAGATTT
AATAAGTGCATCTTTTATAAGAAAAGCCTCTGATGTATTAGCTATAAGAAAAATATTGGAAGAAAATGGTGGAAATGAAA
TACAGATAATCTCTAAGATAGAAAACCAAGAGGGCGTTGACAATATAGATGAGATAATAAAATTTTCTGATGGAATAATG
GTAGCTAGAGGAGATATGGGAGTCGAAATTCCTATAGAAGAAGTTCCAATAGTACAAAAAAGAATAATAGAAAAATGTAA
TAAGGCAGGTAAGCCTGTAATTACAGCAACTCAAATGTTAGATTCTATGATGAGAAATCCTAGACCAACAAGAGCTGAAG
CCTCAGACATAGCCAATGCAATATTTGATGGAACAGATGCCATAATGTTAAGTGGAGAATCAGCAAATGGAAAATATCCA
GTAGAAGCTGCAAGAACTATGTCTAGAATAGCTAAAACAGCAGAAGCAAAATTAAATTATGATGCTATATTAAATAAAAT
GAGAGAAAGTCATATATTAAATGTTCCTAATGCTATAAGCTTATCAGCGTGTACTACAGCATCAGAATTAAATGCTACAG
CTATAATCACAGCAACTCAAAGTGGGCATACTGCAAAAATGGTATCAAAATATAGACCACAATGCCCTATTATAGCAGTA
ACACCAAATGAAATAGTTGCTAGAAAGTTAGCATTAAATTGTGGAGTAGTACCACTATTAACGGAAACATTTAATTCTAC
AGATGAGCTTATAGATAAATCAGTTAATAAATCTTTAGAAGAAGGATATGTTAAAAATGGCGACTTAGTTGTCATAGCAG
GAGGAATACCAGTGTCTTACTCAGGAACAACTAATATGCTAAAAGTTCACATAGTAGGAGATATCTTAGCCCAAGGAAGA
GGTTCAGGAACTAGACCAACTTATGGGAATGTAAAGATAGTAAACAATCCTTCAGAAGCAGAAGATATAGTTGAAAGAGA
TGACATATTAGTAGTTAAAAATTTAGATAGAAGATATATGACTATTTTAGATAGAGTATCAGGCATAGTGGCTGAAGAAG
GTGGAATAACATCACACTTAGCTATAGAATGCATAGCAAGGGATATTCCATTTATATGTAATGCAGGTGGAGCTATGGAT
GTTTTAAAAACAGGAACATATGTAACACTTGATACAGTAAGAGGAATAGTATACAACGGAAGAGCTAATATAGTTTAA

Protein sequence :
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNCGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
GSGTRPTYGNVKIVNNPSEAEDIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-103 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_001783009.1 pyruvate kinase VFG0479 Protein 8e-105 51