Gene Information

Name : polA (CLD_1531)
Accession : YP_001782645.1
Strain : Clostridium botulinum Okra
Genome accession: NC_010516
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3261930 - 3264557 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAAGGAATTCATACTAATGCTATATATGGTTTTGTCAATATGCTTTTAAAAATGAAAGAAGAGATAAAGCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACGTTTAGACATGAAGAATATAAGGACTATAAGGCAGGGCGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAATTTCCTATAGTAAAAGAGCTTTTGTCTGCATTAGCCATAAATATATTCGAAATTGA
TGGATTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCTCTACAATTGGCTACGGATAACGTAAAAGTAGTTATAACTAAAAAGGGCATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGATGGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC
ATCTGACAATATACCTGGAGTACCTGGAATAGGCGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTACTTGAAAACATAGAGAATATAAAAGGGAAAAAATTAAAGGAAAATCTACATGAATATAGAGAGCAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAAAAATGTTTGAAAGGTTAGAATTTAAATCTTTAATGAATAAAGTAGGCAAAAGTGATGAAGAAG
AAACTGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAAAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAATAAAAACATTATA
TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGTTGCTAAATAATACTAAAGAACAATTCATAGAAGTAT
GTAAAGAAATGTTTGAGGGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATACCTAATAGATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCAGAATATTTGTTTAAAAATATAAATAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGAGCAAGATATGGAAGAACTGTTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAAACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAATGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGAA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAAGTAACGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTGGAAGAAGCCGTTGAAAAAGGGTATGTAAGTACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGGGAAAGGCTAGCTATGAATAGTCCAATCCAAGGAAGTGCAGCA
GATATAATAAAATTAGCTATGGTAAATGTTCATAAAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAACGTCCATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAATATAGGTAATAATTGGTATGAAGCAAAGTAA

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEIKPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI
YDKNRMMEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVRKMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK
YKENDLYLYFELEDIVLFSKSRIKTLYANFKDEVYRIDFEMLLNNTKEQFIEVCKEMFEGKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRSNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHKKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 6e-168 45