Gene Information

Name : Bcenmc03_5287 (Bcenmc03_5287)
Accession : YP_001778904.1
Strain :
Genome accession: NC_010515
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase and ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2389477 - 2391129 bp
Length : 1653 bp
Strand : -
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: bur:Bcep18194_B0670 AMP-dependent synthetase and ligase

DNA sequence : 
ATGCAATCCTCCCCTTCCTCCCTGGCGGCGGCCGTGCCGGCCGACGCGCCCGACTGGCCCGACGACTTCGCGCGCCGCTA
CCGCGACGCCGGCTACTGGCAGGACACGACGTTCTTCGACGCGCTCGATGCCTGCGCGCAGCGCCATCCCGACGCGCTCG
CCGTCGTCGACGGCACGCTGCGGCTGCGCTATCGCGATCTGCTCGCGCGCATTCGCCGCCTCGCGGGCGGCCTCGCGCGC
CTCGGCCTGGTACGCGGCGACGCAGTCGTCGTCCAGTTGCCGAACGGCGCGCGTTTCATCGAGACGTGCTTCGCGCTGTT
CCAGCTCGGCGTGCGGCCGGTGCTCGCGCTGCCCGCGCACCGTCACTACGAGATCGGCGCGTTCTGCCGCTTCGCCGGTG
CGCGTGCCTATCTCGGCGCGTCGCAGCTCGGCGATTTCGACTGCCGGCCGCTCGCGGCCGCGTTGCAGGCCGACTGCCCG
ACGCTCGAGCACATCGTGATGGCCGGCGACGATCACGCGTTCACGTCGTTCGACGCGCTGTACGACGCGCCGCCCGTGCT
CGACTGCTCGGCACACGCCGACGACATCGCGTGCTTCCAGCTGTCGGGCGGCACGACCGGCACGCCGAAACTGATTCCGC
GCCGCCATCGCGAATACCTGTACAACGTGCGCGCCTGCAGCGCCGCGAGCGGTTTCGATGCCGACACCGTCTATCTCGCC
GCGCTGCCGATGGCGCACAACTTCACGCTGTGCTGCCCCGGCACGATCGGCGCATTGCTCGCGGGCGGCCGGGTGGTAAC
GACCGCGCGGCCCGAGCCCGACCAGAGCTTCGCGCTGATCGCGCAGGAACGCGTTACGCACACCGCGCTCGTGCCGCCGC
TCGCGCTGCTGTGGCTCGACGAGCAGCCGCAACGGCAGGCCGACCTGTCGAGCCTGCGCGTGCTGCAGGTCGGCGGCGCA
CGGCTGATGGACCACGCGGCCGAACGCGTGACGCCCGTGCTCGGCTGCCGGCTGCAGCAGGTGTTCGGCATGGCCGAAGG
GCTCATCTGCTGCACGCGGCTCGACGATGCGCCCGAGCGCATCGCGCACACACAGGGCCGCCCCGTTTCCGAAGGCGACG
AAGTGCGCATCGTCGACGAAAGCGGCGCACCGGCCGCGGCCGGCGAGATCGGCGAATTGCAGGTACGCGGCCCGTACACG
ATCCGCGGCTACTACCGGCTCGCGGAGCACGACGCGACGGCATTCACGGCCGACGGCTTCTACCGCAGCGGCGACCGCGT
GCGGCGCACCGCGGACGGCGACCTCGTCGTCGAAGGCCGCGACAAGGATCAGATCAACCGCGGCGGCGAGAAGGTCTCCG
CCGAGGAAGTGGAGAACCTGTTGCTCGCGCATCCGCAGGTGCACGATGCGGCGGTCGTCGCGATGCCCGACCCGATCCTC
GGCGAGCGAACCTGCGCGTTCGTGGTCGCACGCGCGCCGGCCCCGTCCAGGCTCGTGCTGAAACGCTACCTGCGCGACCG
CGGGCTCGCGGCGTTCAAAATCCCCGACCGCATCGAATTCATGCCGCGCTTTCCGGAAACCGGCATCGGCAAGACCAGCA
AGAAATCGCTGCGCGACCTGCTGCGCCGCCAGTTGCAGGCGGCGGCCGCATGA

Protein sequence : 
MQSSPSSLAAAVPADAPDWPDDFARRYRDAGYWQDTTFFDALDACAQRHPDALAVVDGTLRLRYRDLLARIRRLAGGLAR
LGLVRGDAVVVQLPNGARFIETCFALFQLGVRPVLALPAHRHYEIGAFCRFAGARAYLGASQLGDFDCRPLAAALQADCP
TLEHIVMAGDDHAFTSFDALYDAPPVLDCSAHADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSAASGFDADTVYLA
ALPMAHNFTLCCPGTIGALLAGGRVVTTARPEPDQSFALIAQERVTHTALVPPLALLWLDEQPQRQADLSSLRVLQVGGA
RLMDHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDAPERIAHTQGRPVSEGDEVRIVDESGAPAAAGEIGELQVRGPYT
IRGYYRLAEHDATAFTADGFYRSGDRVRRTADGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHPQVHDAAVVAMPDPIL
GERTCAFVVARAPAPSRLVLKRYLRDRGLAAFKIPDRIEFMPRFPETGIGKTSKKSLRDLLRRQLQAAAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-98 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-97 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-98 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-98 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-98 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-98 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-98 50
ybtE CAA21394.1 - Virulence HPI Protein 2e-98 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-97 50
irp2E YP_005163485.1 siderophore biosynthetic protein Virulence Not named Protein 2e-64 41
irp2E YP_005134497.1 siderophore biosynthetic protein Virulence Not named Protein 1e-64 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bcenmc03_5287 YP_001778904.1 AMP-dependent synthetase and ligase VFG0168 Protein 2e-163 68
Bcenmc03_5287 YP_001778904.1 AMP-dependent synthetase and ligase VFG0932 Protein 1e-95 45
Bcenmc03_5287 YP_001778904.1 AMP-dependent synthetase and ligase VFG1817 Protein 8e-88 45