Gene Information

Name : lysS (Xfasm12_0457)
Accession : YP_001775098.1
Strain : Xylella fastidiosa M12
Genome accession: NC_010513
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 505575 - 507095 bp
Length : 1521 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAGCATCTCCCTGCGTCGCAGGTGTCCTTTGACGAGAACGCGCTGATTGCCGAGCGCCGTGCAAAACTTTTAGC
ACTACGTGCGCAAGGCGTGGTTTATCCGAATGATGTCAAACGCGAGCATTATGCTGCTGATGTGCAGGCAGCATTTGCCA
ACGTCGAGACATGGACTGCTGAGACCCTGGAGGCGTCAAGCCATCGTGTACGTATGGCTGGCCGATTGATGGCTAAGCGT
CTCATGGGCAAGGCCAGCTTTGCTCAGATTCAGGACGAGTCTGGCCGCATCCAGCTGTTAATACAGAGTAACGTGCTTGG
TGAGGACAGCTATGCCGCATTCAAGGTGTTGGATGTTGGCGACATCATTGCTGTTGAAGGAGGATTGACCCGCACCAGAA
CGGGCGAGCTGTCGGTCAAGGTGAATGTCTTGCGACTTTTGACGAAGGCGTTACGTCCATTGCCTGACAAGTGGCACGGG
CTGACTGATGTGGAGCAGCGCTACCGCCAGCGCTATGTTGATCTTATTGTGACTCCAGAATCGCGTGAGATCTTCATCAA
ACGCTCCAAGATCATTCGTGCGCTGCGTACTTGGCTGGATGCGCGTCTTTTCTTGGAAGTAGAGACGCCGATGATGCATT
ACATCCCAGGAGGTGCTGCGGCTAAGCCGTTTGTGACCTACCACAATGCCTTGGATTTAGAGTTATATCTGCGCGTGGCC
CCAGAGTTGTATCTCAAGCGTTTGGTCGTGGGTGGGCTGGAACGGGTTTACGAGATTAACCGCAACTTCCGTAACGAGGG
GGTGTCGACCCGGCACAACCCCGAATTTACGATGCTTGAGTTATACGAGGCCTATTCCACCTACCATGAGGTCATGGATC
TGGCTGAGACGATGATCCGTGATACTGCACAGTCTGTGTTGGGCACGACCCAGGTGATTTGGGACGGTGCTCAGATTGAT
CTGGGTCCGATATTCCGGCGTTGGCGCATGGATGAGGCAGTTTGCCATCATAATCCTGAGATCAGCGTTGCCGAGTGTAC
CGATCGTGATGCATTGCTCCTTCACTGTGAGAGACTCAAGATCAAGGTTAAATCGTCCTACGGTTGGGGTCGATTGCTCT
TGAGTATTTTTGAGGCGACTGTCGAGCATACGTTGATCCAGCCGACCTTCATTACGGATCATCCGGTTGAAATCTCTCCT
TTAGCGCGCGAAAGCGACATCGAATCAGGGTATACCGATCGCTTCGAACTGTTCATCAATGGGAAGGAAATCGCCAACGG
TTTCTCGGAATTGAACGATCCGGAGGAGCAGGCGATGCGCTTCCAAAAGCAGGTTGAAGCCAAAGAAGGGGGGGATGATG
AGGCCATGTACTACGATGCCGACTATATTCGCGCTTTGGAGTATGGGATGGCGCCAACCGGAGGGTTAGGGATTGGTGTT
GATCGACTGGTGATGCTGCTGACCGGTAGCACCTCGATCCGCGATGTGCTGTTATTCCCTTATATGCGTCCAGAACGTTA
A

Protein sequence :
MTEHLPASQVSFDENALIAERRAKLLALRAQGVVYPNDVKREHYAADVQAAFANVETWTAETLEASSHRVRMAGRLMAKR
LMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVGDIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHG
LTDVEQRYRQRYVDLIVTPESREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIRDTAQSVLGTTQVIWDGAQID
LGPIFRRWRMDEAVCHHNPEISVAECTDRDALLLHCERLKIKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISP
LARESDIESGYTDRFELFINGKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
DRLVMLLTGSTSIRDVLLFPYMRPER

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-128 54
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-79 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001775098.1 lysyl-tRNA synthetase VFG1668 Protein 5e-128 54