Gene Information

Name : Mrad2831_6325 (Mrad2831_6325)
Accession : YP_001766490.1
Strain :
Genome accession: NC_010509
Putative virulence/resistance : Virulence
Product : type IV secretion/conjugal transfer ATPase
Function : -
COG functional category : U : Intracellular trafficking, secretion and vesicular transport
COG ID : COG3451
EC number : -
Position : 22424 - 24913 bp
Length : 2490 bp
Strand : +
Note : TIGRFAM: type IV secretion/conjugal transfer ATPase, VirB4 family; PFAM: CagE TrbE VirB component of type IV transporter system; KEGG: bms:BRA0066 type IV secretion system protein VirB4

DNA sequence :
ATGAATGCACAGGCCAGGTATCAGCAGCAGTTGAACGGCGAGCGCTCGATCTCGCCGTTTATTCCGTACAGCAGCCAGGT
TTCGCCCGACACGATCGTGACGCTCGAAGGCGATTACGTGCGCACCTGGCGGCTCGCCGGGTTGGCGTGGGAGACGGCAG
ACCCGGAAGAACTGTTGCAGCGCAAGGAAGAACTAAACACCCTGTATCGCTCCATCGCGAGCGAGCATGTTGCGATGTGG
ACTCACATCCTCCATCGCAAGACGACCGACCGGCTGGTGTCGCATTTCGACAATGAGTTCTGCCGCCAGCTTGACGAGAA
GTATTACGCCTCGTTCACCGGCTACAAGATGATGGCAAACGAGCTTTACGTCACGCTCGTTTACCGCCCGCATCCTTCAC
GCATCGGCAAGCTGTTTTCCAAGGCAAGCCGTCGTTCGCACGCCGAGATCATCGCGGATCAGCGCAGGGCGCTGCGCCGG
CTCGATGAGCTTGCATCGCAGGTGGAGGCGAGTATGCGCCGCTATGGCGGTGATGAGCGCCGTGGTATCGAGGTGTTGCG
CACTTACGACAAGGGCGGCGCGCTCCATTCCCAGCAGTTGGAGTTCTTGAACTACCTGCTGACTGGTGAGTGGCAGCCGG
TACGCGTGCCGCAGGCGCCGTTGAACGAGTACCTGGGTAATGCCTGGGTATTCGCCGGCACCGAGACGATCGAAATTCGC
ACGCCGGTGTCCACGCGCTTTGCTCGCTGCATCGACTTCAAGGACTACCCGGAGAACACCGAACCGGGCATCCTCAACAT
GCTCATGTATAAGGGCTTTGAGTATGTGGTGACGCAGTCGTTCTCCTTCATGCCCAAGCGCGACGGCGTAGATTTCTTGG
ACCGCCAGGAGAAGCGCTTGCGCAATACGGAGGACGGCAGCGCATCGCAGTTGGCAGCCATGACGAAGGCCAAGGACAGC
CTTATCAACGGTGAGTTTGCAATGGGCGAGTACCATTTTTCGATGATGGTATTCGGCGACAGCATGGAGTCGGTGCGTAG
CAATACCACCGAGGCGATGACGACGTTGCAGAACGAGGGCTTCCTCGCTGCCTTGGTCAACACCGCAACGGACGCGGCGT
TCTTTGCGCAACTGCCCTGTAATTTCCGCTTCCGACCGCGCATCGCCGGACTGACATCGCAGAACTTCGCGTGCTTGGCT
GGTTTCCATAACTTCCGCGCCGGCAAGCGCGACGGCAATCCGTGGGGCCAGGCGCTGACTCTGCTCAAAACGCCGTCCGG
TCAGCCGGCCTATCTGAACTTCCATTTCTCCAAGGGAGACGAGGACAACTACGATCAGAAGTTGCTCGGCAACACGCGCG
TGATTGGTCAGTCCGGCTCGGGCAAGACCGTGCTTTTGAACTTCTGCATGTGCCAGGCGCAGAAGTACAAGCACAACGCG
CCGATGGGCTTGTGCTTCATCTTCTTCGACAAGGATCAGGGCGCGAAGGCCACGATCTTGGCGAGCGGCGGCAAGTACCT
GGCTGTTCGCAACGGCCAGCCGACCGGCTTCAACCCGCTTCAGATGGAGCCCACTGAAGCGAACATCCTGTTCCTTCAGC
GGCTGGTGCGTTCGCTGGTCAGCAGCGGCGGTAATCGCGTGACGACCGAGGACGAGCGCCGCATCGAGCATGCGGTGCGC
ACAGTGATGCGCATGCCGAAGGCGCTGCGTCGGCTCTCAACGGTACTTCAGAACATCACCGAGGGCGGCGACAAGGAAGA
TCGCGAGAACAGCGTGGCGAAGCGCCTCGCGAAGTGGTGTGCCGACGATGGTCATGGCAAGACCGGTTCGCTCGCCTGGG
CGCTGGATTGCGCGCACGATCAAATCGACTTCACCACCCACACGACCTACGGTTTCGATGGCACGGACTTCCTCGACAAC
GAGGACGTTCGCACGCCGATCTCCATGTACCTGCTGCATCGCATGGAGTCGGTCATCGACGGCCGCCGCTTCATCTACTG
GATGGATGAGGCGTGGAAGTGGGTCGATGACGATGCGTTCAGCGACTTCGCCGGCAACAAGCAATTGACGATCCGCAAGC
AGAACGGCCTCGGCGTCTTCGCGACGCAGATGCCGAGTAGCCTGCTGAAGTCGAAGATCGCATCCCAGCTCGTGCAACAG
GTTGCGACCGAGATTTACCTGCCGAACCCGAAGGCCGATTATCACGAGTACGTGGACGGCTTTAAGGTGACTGAAGATGA
GTACAAGATCATCAAGTCAATGAGCGAGGACAGTCGCTTGATGATCGTGAAGCAAGGTCACCAATCGATGATCTGCCGTC
TCGACCTGGGCGGGATGGGTGACGAGCTGGCGATACTGTCCGGTAGTTCGGACAACAACGAGCTGTTGGATGAGATCATC
GCCGAGGTCGGCGACGATCCGAGCCAATGGCTTCCCGTGTTCCACGAGCGCCGCAAGGCCCGCGTGGTGGCTTCCAAGAA
ATCCAACTGA

Protein sequence :
MNAQARYQQQLNGERSISPFIPYSSQVSPDTIVTLEGDYVRTWRLAGLAWETADPEELLQRKEELNTLYRSIASEHVAMW
THILHRKTTDRLVSHFDNEFCRQLDEKYYASFTGYKMMANELYVTLVYRPHPSRIGKLFSKASRRSHAEIIADQRRALRR
LDELASQVEASMRRYGGDERRGIEVLRTYDKGGALHSQQLEFLNYLLTGEWQPVRVPQAPLNEYLGNAWVFAGTETIEIR
TPVSTRFARCIDFKDYPENTEPGILNMLMYKGFEYVVTQSFSFMPKRDGVDFLDRQEKRLRNTEDGSASQLAAMTKAKDS
LINGEFAMGEYHFSMMVFGDSMESVRSNTTEAMTTLQNEGFLAALVNTATDAAFFAQLPCNFRFRPRIAGLTSQNFACLA
GFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHFSKGDEDNYDQKLLGNTRVIGQSGSGKTVLLNFCMCQAQKYKHNA
PMGLCFIFFDKDQGAKATILASGGKYLAVRNGQPTGFNPLQMEPTEANILFLQRLVRSLVSSGGNRVTTEDERRIEHAVR
TVMRMPKALRRLSTVLQNITEGGDKEDRENSVAKRLAKWCADDGHGKTGSLAWALDCAHDQIDFTTHTTYGFDGTDFLDN
EDVRTPISMYLLHRMESVIDGRRFIYWMDEAWKWVDDDAFSDFAGNKQLTIRKQNGLGVFATQMPSSLLKSKIASQLVQQ
VATEIYLPNPKADYHEYVDGFKVTEDEYKIIKSMSEDSRLMIVKQGHQSMICRLDLGGMGDELAILSGSSDNNELLDEII
AEVGDDPSQWLPVFHERRKARVVASKKSN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
trwK YP_001610476.1 TrwK protein Virulence Not named Protein 3e-158 42
trwK CAD42844.1 TrwK protein Not tested trw-PAI Protein 2e-158 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mrad2831_6325 YP_001766490.1 type IV secretion/conjugal transfer ATPase VFG2211 Protein 0.0 73
Mrad2831_6325 YP_001766490.1 type IV secretion/conjugal transfer ATPase VFG0018 Protein 0.0 52
Mrad2831_6325 YP_001766490.1 type IV secretion/conjugal transfer ATPase VFG2259 Protein 5e-158 42