Gene Information

Name : fliI (Mrad2831_5574)
Accession : YP_001758202.1
Strain : Methylobacterium radiotolerans JCM 2831
Genome accession: NC_010505
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 5893672 - 5895024 bp
Length : 1353 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGACACAGGATCCGGCAAGATCCGTTGCTTCCCTCGCGTCGGCGCGGGCCGCCCTGGAGCGGATCGAGGTGCTGGAGAC
GTTCGGGCGGGTCGTGGCGATCCGCGGCCTGCTGGTCGAGGTCGCCGGACCCGTGGCCGCGATGCGACTCGGCGGCCGGG
TCGACGTCGAGGGCGCCAACGGCCACGGGCTGGTCCCGTGCGAGGTCATCGGCTTCCAGGGCGATCGGGCACTCGCGATG
CCGTTCGGCTCGCTCGAAGGCGTGCGGCGCGGCTGCCCGGCCTATGTCCGCGACGAATCGGCCGGCGCGATCCGGCCCTC
CGGCGCGTGGCTGGGCCGGGTGATCGACGCCCTCGGGCGGCCGGTCGACGGGCTCGGCCCCCTGGCGCAGGGGCCCGACG
TCTATCCCCTGCGCGCGGATCCGCCCCCGGCCCATGCCCGCACCCGGGTCGGCGGTCCGCTCGATCTCGGCGTCCGATGC
ATCAACACCTTCCTGACCATGTGCGCGGGCCAGCGGATGGGCATCTTCGCCGGGTCGGGCGTCGGCAAGTCCGTGCTGCT
GTCGATGCTCGCCCGCTACACGGCCGCCGACGTGGCGGTGATCGGCCTCGTGGGCGAGCGCGGCCGCGAGGTGCAGGAAT
TCCTCCAGGACGATCTGGGCGCCGCCGGCCTCGCGCGCTCGGTGGTCGTGGTGGCGACCTCCGACGAGCCGGCCCTGATG
CGCCGGAACGCGGCCTACGTGACCCTGTCGGTCGCCGAGCACTTCCGCGATCAGGGCGCCAAGGTCCTGTGCATGATCGA
CTCGATCACGCGCTTCGCCATGGCCCAGCGCGATATCGGCCTCGCCGCCGGCGAGCCGCCGACCGCCAAGGGCTACACGC
CGACCGTCTTCTCGGAGCTGCCGCGCCTGCTCGAGCGCGCCGGCCCCGGGGTGGGGCAGGGGACGATCTCGGCCCTGTTC
ACCGTCCTGGTCGAGGGCGACGACCACAACGAGCCCGTGGCGGACGCGGTGCGTGGCATCCTCGACGGCCACATCGTCAT
GGAGCGCGCCATCGCGGAGCGCGGGCGCTACCCGGCGATCAACGTGTTGCGCTCGGTCTCGCGCACCATGCCGCGCTCCT
GCGATCCGGCCTATCTCCCGGTCGTGCGGCGCGCGCGGCGGCTGCTGTCGACCTACGCCGACATGGAGGAGCTGATCCGC
CTCGGCGCCTACCGGGCCGGCTCCTCCCAGGAGGTCGACGAGGCGGTCGCGCTCATGCCGCATCTTGAGGCTTTTCTGGG
GCAGGGTAAGGAAGAAGCAACGTCCATCGGCGATGGTTACGCACGGCTCTCCGAGATCGTCGGCGGGGCCTAG

Protein sequence :
MTQDPARSVASLASARAALERIEVLETFGRVVAIRGLLVEVAGPVAAMRLGGRVDVEGANGHGLVPCEVIGFQGDRALAM
PFGSLEGVRRGCPAYVRDESAGAIRPSGAWLGRVIDALGRPVDGLGPLAQGPDVYPLRADPPPAHARTRVGGPLDLGVRC
INTFLTMCAGQRMGIFAGSGVGKSVLLSMLARYTAADVAVIGLVGERGREVQEFLQDDLGAAGLARSVVVVATSDEPALM
RRNAAYVTLSVAEHFRDQGAKVLCMIDSITRFAMAQRDIGLAAGEPPTAKGYTPTVFSELPRLLERAGPGVGQGTISALF
TVLVEGDDHNEPVADAVRGILDGHIVMERAIAERGRYPAINVLRSVSRTMPRSCDPAYLPVVRRARRLLSTYADMEELIR
LGAYRAGSSQEVDEAVALMPHLEAFLGQGKEEATSIGDGYARLSEIVGGA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-78 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-78 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-78 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-78 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-78 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-79 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-79 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-80 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-80 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-80 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-65 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-65 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-65 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-64 46
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-64 46
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 4e-70 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-65 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-65 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 7e-64 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 6e-64 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 3e-61 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 9e-63 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-67 43
escN AAK26715.1 EscN Virulence LEE Protein 3e-67 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-67 43
escN AAL57542.1 EscN Virulence LEE Protein 3e-67 43
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-67 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-67 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-68 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-68 42
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-60 42
unnamed AAL06369.1 EscN Virulence LEE Protein 9e-68 41
escN AAC31513.1 L0034 Virulence LEE Protein 8e-68 41
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-68 41
escN AAC38383.1 EscN Virulence LEE Protein 2e-68 41
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-67 41
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-67 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 8e-59 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 8e-59 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-66 41
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-67 41
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-58 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-58 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-58 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-58 41
invC AAS66861.1 InvC Not tested SSR-2 Protein 4e-53 41
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-51 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001758202.1 flagellum-specific ATP synthase VFG2499 Protein 5e-83 50
fliI YP_001758202.1 flagellum-specific ATP synthase VFG2331 Protein 2e-82 47
fliI YP_001758202.1 flagellum-specific ATP synthase VFG2144 Protein 9e-83 47
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0192 Protein 1e-78 47
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0048 Protein 5e-81 46
fliI YP_001758202.1 flagellum-specific ATP synthase VFG1254 Protein 1e-81 46
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0515 Protein 6e-65 46
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0390 Protein 2e-80 45
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0812 Protein 4e-68 41
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0730 Protein 1e-68 41
fliI YP_001758202.1 flagellum-specific ATP synthase VFG0555 Protein 9e-59 41