Gene Information

Name : PputW619_2338 (PputW619_2338)
Accession : YP_001749207.1
Strain : Pseudomonas putida W619
Genome accession: NC_010501
Putative virulence/resistance : Resistance
Product : putative mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 2611169 - 2612854 bp
Length : 1686 bp
Strand : -
Note : TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; pyridine nucleotide-disulphide oxidoreductase dimerisation region; FAD dependent oxidoreductase; Heavy metal transport/detoxification protein; KEGG: rme:Rmet_6

DNA sequence :
ATGACCCATCTAAAAATCACCGGCATGACTTGCGACTCGTGCGCGGCGCACGTCAAGGAAGCGCTGGAAAAAGTGCCAGG
CGTGCAGTCGGCGCTGGTGTCCTATCCGAAGGGCACAGCGCAACTCGCCATCGTGCCGGGCACATCGCCGGACGCGCTGA
CTGCCGCCGTGGCCGGACTGGGCTACAAGGCAACGCTAGCCGATGCGCCACTGGCGGACAACCGCGTCGGACTGCTCGAC
AAGGTGCGGGGATGGATGGCCGCCGCCGAAAAGCACAGTGGCAACGAGCCCCCGGTGCAGGTAGCGGTCATTGGCAGCGG
TGGAGCCGCGATGGCGGCGGCGCTGAAGGCCGTCGAGCAAGGCGCGCAGGTCACGCTGATCGAGCGCGGCACCATCGGCG
GCACCTGCGTCAATGTCGGCTGTGTGCCGTCCAAGATCATGATCCGCGCCGCCCACATCGCCCATCTGCGCCGGGAAAGC
CCGTTCGATGGCGGTATTGCGGCAACTGTGCCTACGATTGACCGCAGTAAGCTGCTGGCCCAGCAGCAGGCCCGCGTCGA
CGAACTGCGGCACGCCAAGTACGAAGGCATCCTGGGCGGTAATCCGGCCATCACCGTTGTGCACGGTGAGGCGCGCTTCA
AGGACGACCAGAGCCTTACCGTCCGTTTGAACGAGGGTGGCGAGCGCGTCGTGATGTTCGACCGCTGCCTGGTCGCCACG
GGTGCCAGCCCGGCGGTCCCGCCGATTCCGGGGTTGAAAGAGTCACCCTACTGGACTTCCACCGAGGCCCTGGCGAGCGA
CACCATTCCCGAACGCCTTGCCGTAATCGGCTCGTCGGTGGTGGCGCTGGAGCTGGCGCAAGCCTTTGCCCGGCTGGGCA
GCAAGGTCACGGTCCTGGCGCGCAATACCTTGTTCTTCCGTGAAGACCCGGCCATCGGCGAGGCGGTGACAGCCGCTTTC
CGTGCCGAGGGCATCGAGGTGCTGGAGCACACGCAAGCCAGCCAGGTCGCCCATATGGACGGTGAATTCGTGCTGACCAC
CACGCACGGTGAATTGCGCGCCGACAAACTGCTGGTTGCCACCGGTCGGACACCGAACACGCGCAGCCTCGCGCTGGACG
CAGCGGGGGTCACTGTCAATGCGCAAGGTGCCATCGTCATCGACCAAGGCATGCGCACGAGCAACCCGAACATCTACGCG
GCCGGCGACTGCACCGACCAGCCGCAGTTCGTCTATGTGGCGGCAGCGGCCGGCACCCGTGCCGCGATCAACATGACCGG
CGGCGATGCGGCGCTCGACCTGACCGCAATGCCGGCCGTGGTGTTCACCGATCCGCAAGTGGCGACCGTGGGCTACAGCG
AGGCGGAAGCCCACCACGACGGGATCGAGACCGACAGCCGCACCTTGACCTTGGACAACGTGCCGCGTGCGCTCGCCAAC
TTCGACACACGCGGCTTCATCAAGTTGGTTATCGAGGAAGGCAGCCATCGGCTGATCGGCGTACAGGCGGTCGCGCCGGA
AGCGGGTGAACTGATCCAGACGGCGGCTCTGGCCATTCGCAACCGCATGACGGTGCAGGAACTGGCCGACCAGTTGTTCC
CCTACCTGACGATGGTCGAGGGGTTGAAGCTCGCGGCGCAGACCTTCAACAAGGATGTGAAGCAGCTTTCCTGCTGCGCC
GGGTGA

Protein sequence :
MTHLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAIVPGTSPDALTAAVAGLGYKATLADAPLADNRVGLLD
KVRGWMAAAEKHSGNEPPVQVAVIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
PFDGGIAATVPTIDRSKLLAQQQARVDELRHAKYEGILGGNPAITVVHGEARFKDDQSLTVRLNEGGERVVMFDRCLVAT
GASPAVPPIPGLKESPYWTSTEALASDTIPERLAVIGSSVVALELAQAFARLGSKVTVLARNTLFFREDPAIGEAVTAAF
RAEGIEVLEHTQASQVAHMDGEFVLTTTHGELRADKLLVATGRTPNTRSLALDAAGVTVNAQGAIVIDQGMRTSNPNIYA
AGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRTLTLDNVPRALAN
FDTRGFIKLVIEEGSHRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCA
G

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA AGK07021.1 MerA Not tested SGI1 Protein 0.0 94
merA AGK07079.1 MerA Not tested SGI1 Protein 0.0 94
merA ABQ57371.1 MerA Not tested SGI1 Protein 0.0 94
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 0.0 94
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 0.0 94
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 0.0 86
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 0.0 86
merA AFG30120.1 MerA Not tested PAGI-2 Protein 0.0 86
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 0.0 80
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 0.0 76

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PputW619_2338 YP_001749207.1 putative mercuric reductase BAC0652 Protein 0.0 99
PputW619_2338 YP_001749207.1 putative mercuric reductase BAC0653 Protein 0.0 81
PputW619_2338 YP_001749207.1 putative mercuric reductase BAC0650 Protein 2e-174 71