Gene Information

Name : EcSMS35_4465 (EcSMS35_4465)
Accession : YP_001746394.1
Strain : Escherichia coli SMS-3-5
Genome accession: NC_010498
Putative virulence/resistance : Unknown
Product : IS3 family transposase orfB
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2801
EC number : -
Position : 4558464 - 4559210 bp
Length : 747 bp
Strand : +
Note : identified by match to protein family HMM PF00665

DNA sequence :
TTGTGCAGCGAAATACGCCGGGCATGGAACCAGAGCCGGGGCTCTGCAGGCGCGCGCACGCTGGCTGAAATGCTGACCCA
AAACGGCGTCCCGATGAGCCGTTATCGTGCCGGACGGCTGATGAAATACCTGAACCTGAGCAGTTGTCAGCCCGGAAAAC
ATCAATACAAAAATGCCTGTCAGGAGCATACGTGTTTGCCGAATCTGCTCGAGCGTCAGTTCGCTGTACCGGAGCCAGAC
AGGGTATGGTGCGGAGATATTACGTATATCTGGGCAGGAAATCGCTGGTGCTATCTGGCGGTCGTCATGGATCTTTTTGC
CCGCAGGGTTATCGGCTGGAGCCTGTCAGCGAATGCCGATACTGCACTGATAAGCAGTGCCTTGCGGATGGCGTATGAGG
TGCGTGGTCAGCCGCGGGACGTCATGTTCCATAGCGACCAGGGAAGTCAGTATACAGGACTGAAATATCAACAGCTTCTC
TGGCGTTACAGGATAAAGCAAAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCTATGGAACGCTTCTTCCGCAG
TCTGAAAACAGAATGGGTGCCGACGGATGGTTACGTAGGCAAGGACGAGGCCCGGCAACAAATTAGCGGTTATATCCTGA
ATTATTACAATAGCGTCAGGCCTCACCATTACAACGGTGGGTTGACGCCGGAAGAATCAGAGAACAGATACCGTTTTTAC
TGTAAAACCGTGGCCAATATTACTTGA

Protein sequence :
MCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNACQEHTCLPNLLERQFAVPEPD
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLL
WRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFY
CKTVANIT

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
Z5089 NP_290241.1 transposase Not tested LEE Protein 2e-101 98
unnamed AAC31485.1 L0006 Not tested LEE Protein 1e-97 98
unnamed ACU09432.1 IS911 transposase orfB Not tested LEE Protein 1e-101 98
ECs4537 NP_312564.1 hypothetical protein Not tested LEE Protein 1e-97 98
ECO111_3779 YP_003236114.1 putative IS602 transposase OrfB Not tested LEE Protein 1e-108 95
unnamed CAI43898.1 putative transposase Not tested LEE Protein 1e-98 95
aec65 AAW51748.1 Aec65 Not tested AGI-3 Protein 1e-98 95
overlap with orfA characteristic of IS3 family members AGK06947.1 IS1359 transposase; OrfB Not tested SGI1 Protein 7e-75 65
orfB AGK06993.1 IS1359 transposase; OrfB Not tested SGI1 Protein 7e-75 65
orfB AGK07051.1 IS1359 transposase; OrfB Not tested SGI1 Protein 7e-75 65
orfB ACX47958.1 IS1359 transposase; OrfB Not tested SGI1 Protein 7e-75 65
VC1789 NP_231424.1 transposase OrfAB subunit B Not tested VPI-2 Protein 9e-75 65
VPI2_0008c ACA01825.1 transposase OrfAB subunit B Not tested VPI-2 Protein 7e-75 65
orfB YP_001217329.1 transposase OrfAB subunit B Not tested VPI-2 Protein 9e-75 65
orfB AGK06910.1 IS1359 transposase; OrfB Not tested SGI1 Protein 7e-75 65
l7094 CAD33743.1 ORF B protein Not tested PAI I 536 Protein 1e-68 61
orfB CAE85178.1 OrfB protein, IS911 Not tested PAI V 536 Protein 1e-68 61
RS04 AAP82949.1 putative transposase Not tested PAPI-2 Protein 3e-63 55

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
EcSMS35_4465 YP_001746394.1 IS3 family transposase orfB VFG0785 Protein 7e-102 98
EcSMS35_4465 YP_001746394.1 IS3 family transposase orfB VFG1122 Protein 4e-75 65
EcSMS35_4465 YP_001746394.1 IS3 family transposase orfB VFG1484 Protein 6e-69 61