Gene Information

Name : EcSMS35_3126 (EcSMS35_3126)
Accession : YP_001745140.1
Strain : Escherichia coli SMS-3-5
Genome accession: NC_010498
Putative virulence/resistance : Unknown
Product : IS66 family transposase orfB
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 3211704 - 3213278 bp
Length : 1575 bp
Strand : +
Note : identified by match to protein family HMM PF03050

DNA sequence :
ATGGACACCTCACTTGCTCATGAGAACGCCCGCCTGCGGGCACTGTTGCAGACGCAACAGGACACCATCCGCCAGATGGC
TGAATACAACCGCCTGCTCTCACAGAGGGTGGCGGCTTATGCTTCTGAAATCAACCGGCTGAAGGCGCTGGTTGCGAAAC
TGCAACGTATGCAGTTCGGTAAAAGCTCAGAAAAACTTCGTGCAAAAACCGAACGGCAGATACAGGAAGCACAGGAGCGA
ATCAGCGCACTTCAGGAAGAAATGGCGGAAACGCTGGGTGAGCAATATGACCCGGTACTGCCATCCGCCGCCCTGCGTCA
GTCTTCAGCCTGTAAACAGTTACCGGCCTCACTTCCCCGTGAAACCCGGGTTATCCGGCCGGAAGAGGAATGCTGTCCTG
CCTGTGGTGGTGAACTCAGTTCTCTGGGATGTGATGTGTCAGAGCAACTGGAGCTTATCAGCAGCGCCTTTAAGGTTATC
GAAACACAACGTCCGAAACAGGCCTGTTGCCGGTGCGACCATATCGTGCAGGCACCAGTACCTTCAAAACCCATTGCACG
CAGTTATGCCGGAGCGGGGCTTCTGGCCCATGTTGTCACCGGGAAATATGCAGACCATCTGCCGTTATACCGCCAGTCAG
AAATATACCGTCGTCAGGGAGTGGAGCTGAGCCGTGCCACACTGGGGCGCTGGACAGGTGCTGTTGCTGAACTGCTGGAG
CCGCTGTATGACGTCCTGCGCCAGTATGTGCTGATGCCCGGTAAAGTCCATGCTGATGATATCCCCGTCCCGGTCCAGGA
GCCGGGCAGCGGTAAAACCCGGACAGCCCGGCTGTGGGTCTACGTCCGTGATGACCGTAACGCCGGTTCACAGATGCCCC
CGGCGGTCTGGTTCGCGTACAGTCCGGACCGGAAAGGTATCCATCCACAAAATCACCTGGCCGGTTACAGCGGTGTGCTT
CAGGCCGATGCTTACGGTGGTTACCGGGCGTTATACGAATCCGGCAGAATAACGGAAGCCGCGTGTATGGCTCATGCCCG
GAGAAAAATCCACGATGTGCATGCAAGAGCGCCCACCTACATCACCACGGAAGCCCTGCAGCGTATCGGTGAACTGTATG
CCATCGAGGCAGAGGTCCGGGGCTGTTCAGCAGAACAGCGTCTGGCGGCAAGAAAAGCCAGAGCCGCGCCACTGATGCAG
TCACTGTATGACTGGATACAGCAACAGATGAAAACACTGTCGCGTCACTCAGATACGGCAAAAGCGTTCGCATACCTGCT
GAAACAGTGGGATGCACTGAACGTGTACTGCAGTAATGGCTGGGTGGAAATCGACAACAACATCGCAGAGAACGCCTTAC
GGGGAGTGGCCGTAGGCCGGAAAAACTGGATGTTCGCGGGTTCCGACAGCGGTGGTGAACATGCGGCGGTGTTGTACTCG
CTGATCGGCACATGCCGTCTGAACAATGTGGAGCCAGAAAAGTGGCTGCGTTACGTCATTGAACATATCCAGGACTGGCC
GGCAAACCGGGTACGCGATCTGTTGCCCTGGAAAGTTGATCTGAGCTCTCAGTAA

Protein sequence :
MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQER
ISALQEEMAETLGEQYDPVLPSAALRQSSACKQLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVI
ETQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVELSRATLGRWTGAVAELLE
PLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVL
QADAYGGYRALYESGRITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYS
LIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
aec53 AAW51736.1 Aec53 Not tested AGI-3 Protein 0.0 99
c3563 NP_755438.1 hypothetical protein Not tested PAI I CFT073 Protein 2e-115 67
ECO103_3554 YP_003223421.1 hypothetical protein Not tested LEE Protein 1e-137 63
Z4317 NP_289543.1 hypothetical protein Not tested OI-122 Protein 8e-138 63
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 2e-124 61
tnp AEA34686.1 transposase Not tested Not named Protein 2e-130 60
ECs4547 NP_312574.1 hypothetical protein Not tested LEE Protein 5e-129 59
unnamed ACU09439.1 IS66 family element transposase Not tested LEE Protein 4e-129 59
Z4337 NP_289562.1 hypothetical protein Not tested OI-122 Protein 2e-129 59
unnamed AAC31494.1 L0015 Not tested LEE Protein 2e-129 59
Z5098 NP_290249.1 prophage-associated protein Not tested LEE Protein 2e-129 59
unnamed CAC39285.1 hypothetical protein Not tested LPA Protein 2e-129 59
Z1131 NP_286666.1 hypothetical protein Not tested TAI Protein 1e-128 59
Z1570 NP_287074.1 hypothetical protein Not tested TAI Protein 1e-128 59
unnamed CAI43806.1 hypothetical protein Not tested LEE Protein 4e-129 59
unnamed AAL57570.1 unknown Not tested LEE Protein 1e-129 59
unnamed AAL08460.1 unknown Not tested SRL Protein 5e-89 57
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 6e-108 54
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 4e-108 54
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 4e-108 54
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 1e-118 53
tnp AEA34687.1 IS66 family transposase Not tested Not named Protein 1e-69 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
EcSMS35_3126 YP_001745140.1 IS66 family transposase orfB VFG1700 Protein 7e-116 67
EcSMS35_3126 YP_001745140.1 IS66 family transposase orfB VFG0793 Protein 1e-129 59
EcSMS35_3126 YP_001745140.1 IS66 family transposase orfB VFG1051 Protein 3e-89 57
EcSMS35_3126 YP_001745140.1 IS66 family transposase orfB VFG1736 Protein 2e-87 54