Gene Information

Name : lfiI (EcSMS35_0253)
Accession : YP_001742360.1
Strain : Escherichia coli SMS-3-5
Genome accession: NC_010498
Putative virulence/resistance : Virulence
Product : lateral flagellar export/assembly protein LfiI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 266475 - 267812 bp
Length : 1338 bp
Strand : +
Note : identified by similarity to GB:CAH19125.1; match to protein family HMM PF00006; match to protein family HMM TIGR01026; match to protein family HMM TIGR03496

DNA sequence :
GTGAGTGATTTTTCCGCTTTCGATAACGCCCTGCGCTCGCTGGAGTCGATCCCGCTGGCCCGCGTCGCCGGGCGGCTGGT
GCGCCTTAATGGCATCCTGCTGGAGAGCGTCGGCTGCCCGCTAATGACCGGGCAGCTGTGTCGCATCGAAAGCGCCAACC
ACACGCTTATCGACGCGCAGGCCGTAGGCTTTAATCGCGATATCACTTACTTAATGCCGTTTAAACAGCCGGTCGGCCTG
ATGGCGGGGGCGCGCGTCTTCCCGGAAGAAAAAGCCCACGACATCCTGATTGGCGAAAGCTGGTTAGGCCGCGTGGTTAA
CGGCCTCGGCGAACCGCTGGATGGCAAAGGCCGCCTGAACGGCAACGACCTTCTGCCGCCGCTGCCGCCGTCGGTTAATC
CCTTAACCCGCCGTTCGGTGGACGAACCGCTGGACGTGGGCGTGAAAGCGATCAACGGCCTGCTGACCATCGGCAAAGGC
CAGCGTGTCGGGCTGATGGCGGGCAGCGGCGTCGGGAAAAGCGTGCTGCTGGGGATGATCACCCGCCAGACCAAAGCCGA
CATCGTGGTGGTCGGGCTGATTGGCGAACGCGGGCGCGAAGTGAAAGAGTTTATCGACCACTCACTGGGCGCGGACGGGC
TGGCGAAGTCTATCGTCGTCGTCGCGCCCGCCGATGAATCGCCGCTGATGCGCCTGAAAGCCACCGAACTGTGCCACTCC
GTCGCCGCCTGGTTTCGCGACCGCGGGCATCACGTTTTACTGCTGGTGGATTCCCTGACCCGCTATGCGATGGCGCAGCG
TGAAATTGCGCTCTCGCTTGGCGAACCACCCGCCACCAAAGGCTATCCGCCGTCGGCATTCGGCATGATCCCCAAACTGG
TCGAAAGCGCAGGCAACAGCGAAAGCGCGGGATCGATGACTGCCATCTACACCGTGCTGGCGGAAGGCGACGACCAGCAA
GATCCGATTGTCGACTGCGCCCGTGCGGTGCTGGACGGGCATATCGTATTAACCCGCAAACTGGCGGAAGCGGGGCATTA
TCCGGCCATTGATATCGGCCAGTCGATCAGCCGCTGCATGAACCAGGTCACCAGCCTGGAACATCAGCAATCCGCCCGCG
CGTTAAAGCAAAACTACGCCGCCTACATGGAGATCAAACCGCTGATCCCACTCGGCGGTTATGTGGCAGGCGCGGACGCC
AGCGTCGACAAAGCGGTGAAAATGTTCCCGGCTATCGAGCGTTTTTTGCGCCAGGAGATGCGCGAACCCGCCTCCCTCGA
ACTGGTGCAAAGCCGCCTGCAAATTCTCTTTCCTGGCGTCAAAAAGGCAGAACAGTAA

Protein sequence :
MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTGQLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGL
MAGARVFPEEKAHDILIGESWLGRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSIVVVAPADESPLMRLKATELCHS
VAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEPPATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQ
DPIVDCARAVLDGHIVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLGGYVAGADA
SVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 8e-75 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 4e-75 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-73 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-72 45
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-83 45
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-84 45
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-85 45
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-83 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-71 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-71 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-79 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-67 44
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-83 44
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-83 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-83 44
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-83 44
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-83 44
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-82 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-72 44
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-72 43
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-72 43
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-72 43
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-72 43
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 8e-73 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-69 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 5e-69 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-69 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-71 42
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-71 42
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-71 42
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 9e-71 42
escN AAK26715.1 EscN Virulence LEE Protein 5e-71 42
escN AAL57542.1 EscN Virulence LEE Protein 5e-71 42
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-70 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-69 42
escN AAC31513.1 L0034 Virulence LEE Protein 4e-71 41
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-71 41
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-70 41
escN AAC38383.1 EscN Virulence LEE Protein 5e-71 41
escN NP_290268.1 hypothetical protein Virulence LEE Protein 5e-71 41
ECs4568 NP_312595.1 EscN Virulence LEE Protein 5e-71 41
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-70 41
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-70 41
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-57 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 9e-57 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-57 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-57 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG1254 Protein 6e-109 57
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG2499 Protein 3e-102 54
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG2331 Protein 5e-100 52
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0390 Protein 6e-79 44
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0192 Protein 5e-83 44
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0048 Protein 3e-85 43
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0515 Protein 4e-73 43
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG2144 Protein 4e-80 41
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0812 Protein 2e-71 41
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0730 Protein 3e-71 41
lfiI YP_001742360.1 lateral flagellar export/assembly protein LfiI VFG0555 Protein 4e-57 41