Gene Information

Name : fliI (Bsph_1553)
Accession : YP_001697281.1
Strain : Lysinibacillus sphaericus C3-41
Genome accession: NC_010382
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1644482 - 1645807 bp
Length : 1326 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAAGACGGCTCAATTAATCGAACATATTCCTCATATGCCAACATTTAAAAAATTTGGTAGAGTCACTAGAGTTGTCGG
CTTGATGATTGAGTCGCAAGGTCCAGATAGTTCCATAGGTGATGTTTGTAAAATTCATGTGGAAACTGTAAACAATGGTC
ACCAAATCATTTTAGCTGAAGTTGTGGGCTTTAAAGATGAAATTGTTGTTTTAATGCCTTTCACTTCTTTAAGAGAAATA
TCTATTGGTTGTCTTGTTGAAGGGACGGGGGCTCCACTAGAAGTAAAAGTGGGCCCAGAGCTAATAGGGAAAGTGTTAGA
TTCTATGGGTAATCCTATTGATGGCACCTTGCTACCAAAAGGGCTAATGACGGTTCCGACAGAGCAAGATCCACCAAATC
CACTGACACGTCCACCTATTAACGAAAGACTTGAAGTGGGCGTAAAGGCTATTGATGGTATGCTGACAGTTGGTAATGGG
CAACGTGTCGGGATTTTCGCTGGTTCAGGGGTCGGAAAAAGTACACTTCTTGGAATGATTGCGAGGAATACACAAGCAGA
TTTGAATGTAATTGCTCTGATCGGAGAACGTGGACGTGAGGTCCGTGAATTTATTGAACGTGATTTAGGCCCCGAAGGTC
TAAGTCGGTCCATAGTTGTGGCTGCTACTTCTGACCAACCGGCATTGATGAGGATAAAAGGGGCGTTTACTGCAACAGCA
ATCGCCGAGTATTTTAGAAATCGTGGTTTAAATGTCATGTTGATGATGGACTCTGTAACACGTGTTGCCATGGCTCAGCG
TGAAATTGGCCTTGCCACAGGAGAGCCGCCGGCACAGAAGGGATATACCCCATCTGTATTTGCGATATTACCTAAATTAC
TAGAACGTACAGGTACTAATGAAAAGGGTTCTATCACTGCATTTTATACGGTCTTAGTAGATGGTGATGATATGAATGAA
CCAATCGCTGATACGGTGCGAGGGATATTAGATGGTCATATTGTGCTTGATCGAAATCTAGCAAATAAAGGGCAATACCC
TGCAATAAACGTCTTAAAAAGTGTTAGTCGTTTGATGAATCATGTGGCAGCGCCAGAGCATAAAAAAGCTGCTGAACGAT
TAAGAGAACTCTATTATACATACGACAAATCTGAGGATTTGATCAATATTGGCGCTTATAAACGTGGGACGTCTAGAGAA
ATTGATGAAGCTATTCATTATGAACCGCTTATAACTACTTATCTCAAACAGGGTTATTTGGACAAAGTGACTTTGGAAGA
AAGTGTGAATGAGCTACTATCCTTATCGAACGGTGGTGTAAACTAG

Protein sequence :
MKTAQLIEHIPHMPTFKKFGRVTRVVGLMIESQGPDSSIGDVCKIHVETVNNGHQIILAEVVGFKDEIVVLMPFTSLREI
SIGCLVEGTGAPLEVKVGPELIGKVLDSMGNPIDGTLLPKGLMTVPTEQDPPNPLTRPPINERLEVGVKAIDGMLTVGNG
QRVGIFAGSGVGKSTLLGMIARNTQADLNVIALIGERGREVREFIERDLGPEGLSRSIVVAATSDQPALMRIKGAFTATA
IAEYFRNRGLNVMLMMDSVTRVAMAQREIGLATGEPPAQKGYTPSVFAILPKLLERTGTNEKGSITAFYTVLVDGDDMNE
PIADTVRGILDGHIVLDRNLANKGQYPAINVLKSVSRLMNHVAAPEHKKAAERLRELYYTYDKSEDLINIGAYKRGTSRE
IDEAIHYEPLITTYLKQGYLDKVTLEESVNELLSLSNGGVN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-97 53
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-101 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-101 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-102 52
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-101 52
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 8e-92 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-101 51
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-101 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-98 51
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-98 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-84 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 5e-84 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-84 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 6e-84 48
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-84 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 8e-84 48
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-79 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-79 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-82 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-82 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-82 48
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-82 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 5e-83 48
escN AAC31513.1 L0034 Virulence LEE Protein 4e-83 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 5e-83 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-83 48
escN AAC38383.1 EscN Virulence LEE Protein 1e-83 48
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-82 48
escN AAK26715.1 EscN Virulence LEE Protein 6e-82 48
escN AAL57542.1 EscN Virulence LEE Protein 6e-82 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-75 45
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-80 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-81 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-74 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-76 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 9e-74 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 7e-74 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-74 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 9e-76 43
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-75 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 6e-70 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-74 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-74 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 6e-72 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001697281.1 flagellum-specific ATP synthase VFG2144 Protein 2e-103 53
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0048 Protein 2e-95 52
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0390 Protein 1e-96 52
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0192 Protein 1e-97 52
fliI YP_001697281.1 flagellum-specific ATP synthase VFG1254 Protein 8e-93 52
fliI YP_001697281.1 flagellum-specific ATP synthase VFG2499 Protein 5e-87 49
fliI YP_001697281.1 flagellum-specific ATP synthase VFG2331 Protein 7e-89 48
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0515 Protein 3e-84 48
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0730 Protein 7e-84 48
fliI YP_001697281.1 flagellum-specific ATP synthase VFG0812 Protein 2e-83 48
fliI YP_001697281.1 flagellum-specific ATP synthase VFG1008 Protein 2e-71 42
fliI YP_001697281.1 flagellum-specific ATP synthase VFG2459 Protein 1e-75 42