Gene Information

Name : PputGB1_5192 (PputGB1_5192)
Accession : YP_001671410.1
Strain : Pseudomonas putida GB-1
Genome accession: NC_010322
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 5805598 - 5807850 bp
Length : 2253 bp
Strand : -
Note : TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; heavy metal translocating P-type ATPase; PFAM: Haloacid dehalogenase domain protein hydrolase; E1-E2 ATPase-associated domain protein; KEGG: pen:PS

DNA sequence :
ATGAACCAGCCTGTCAGCCACGACCACAAGCACTCGCACGATCACGCCCATGGCGATGACGACCACGGCCATGCTGCCCA
CGGCCACAGCTGCTGCGGCTCCAAAGCCGCGCCATCGCTGGTGCAACTGAGCGAAACGGCCAGTGCCCAGGCGCAGCTCA
GCCGTTTTCGCATCGACGCCATGGACTGCCCCACCGAGCAGACGCTGATCCAGGACAAACTGGGCAAGCTGGCCGGAATC
GAGCAGCTGGAATTCAACCTGATCAACCGCGTGCTTGGCGTGCGCCATACCTTGAACGGTACGGCTGAAATCGAGCGGGC
CATCGACAGCCTGGGCATGAAGGCCGAGCCGATTGCTGCCGAGGACGACGGCACCACCAGCGTGGCACAACCGGCCAAGA
CCCGCTGGTGGCCGCTGGCACTGTCGGGTATTGCTGCGATTACCGCCGAAATCGTGCACTTCGCTGCGCTGGCCCCGGAA
TGGGTGGTAGCGGGGCTGGCGCTGTTGGCGATCCTCGGCTGCGGCCTGGGTACCTACAAGAAGGGCTGGATCGCCCTGAA
AAACCGCAACCTCAACATCAATGCCTTGATGAGCATTGCCGTGACCGGTGCGGTGCTGATCGGCCAGTGGCCGGAAGCAG
CCATGGTGATGGTGCTGTTCACGGTTGCCGAGCTGATCGAAGCGCGCTCGCTGGACCGTGCGCGCAATGCCATCGGCGGG
TTGATGCAGTTGGCCCCGGACATGGCCACGGTGCAGCAGGCCGATGGCCTGTGGCGCGAGCTGGACGTGCGTGAAGTGGC
CATCGGTGCGTTGGTGCGGGTGCGCCCCGGCGAACGCATCGGCCTGGACGGTGAAGTGACCAGTGGGCAGTCCAGCGTCG
ATCAGGCACCGATCACCGGTGAAAGCCTGCCTGTAGAGAAGGCAGTGGGCGACAAACTGTTCGCCGGTACCATCAACCAG
GCGGGAGCATTGGAGTTCCGCGTTACCGCTGCTGCCGGGCAGTCTACCCTGGCGCGGATCATCAAGGCCGTGGAGGAGGC
ACAAGGCGCGCGGGCACCTACCCAGCGCTTCGTCGACCGTTTTTCGCGCATCTATACCCCGGTGGTGTTCGCCATTGCCC
TGGCCGTGGCGGTGATCCCGCCGTTGTTCATGGCCGGTGCCTGGTTCGACTGGGTCTACCGTGCTCTGGTATTGCTGGTG
GTTGCGTGCCCCTGCGCATTGGTGATCTCGACCCCGGTGACCATCGTCAGCGGCCTGGCGGCTGCCGCGCGCAAAGGCAT
CCTGATCAAGGGCGGCGTCTATCTGGAGGGCGGCCGCCACCTGGACTTCCTGGCCCTGGACAAGACCGGCACCATCACCC
ACGGCAAACCGGTACAGACTGACGCCGAAGTGCTGGCGCCGCAGTTCGAAGCCCGCGCTCAAGCCCTGGCCGCCAGCCTG
GGCGAGCGCTCGGACCACCCGGTGTCCCGCGCTATCGCTCAGTTCGGCAAGGCGCAAGGGCTGGCTCTAAGTGAAGTGGA
TGACTTCGCCGCCCTGGGCGGGCGCGGTGTGCGTGGCACCATCGACGGTGAGGTCTACCACTTGGGCAACCACCGCCTGG
TCGAAGAGCTGGGCCTGTGCTCGCCAGCGCTGGAAGCCAAACTGGATGCGCTGGAGCGTCAGGGCAAGACCGTGGTGCTG
CTGCTCGACCGTTCCGGGCCATTGGCGCTGTTTGCCGTTGCCGACACCGTGAAGGACAGCAGCCGCCAGGCTATCGCCGA
GCTGCATGAACTGGGTATCAAGACTGTCATGCTGACCGGTGACAATCCGCATACCGCCCAGGCCATCGCCGCTGTGGTGG
GCATCGACCGCGCCGAGGGTAACCTGCTGCCAGCCGACAAGCTGAAAACCATCGAGGCCTTGTACGCAGAAGGCCACCGG
GTGGGCATGGTCGGTGATGGTATCAACGACGCGCCAGCTCTGGCCCGCGCCGAGATCGGTTTTGCCATGGCCGCCGCTGG
TACCGACACCGCCATCGAGACCGCCGACGTAGCGCTGATGGACGACGACTTGCGGAAAATTCCGGCCTTCGTCAGGCTGT
CGCGCCAAAGCGCGGCGATCCTCATGCAGAACATCGTTCTGGCGTTGGGCATCAAGGCGATATTCCTGGCCATCACCTTT
GCTGGCATGGCGACCATGTGGATGGCGGTGTTCGCCGACATGGGCGTGAGCCTGCTGGTGGTGTTCAACGGCTTGCGCCT
GTTGCGCAAATAG

Protein sequence :
MNQPVSHDHKHSHDHAHGDDDHGHAAHGHSCCGSKAAPSLVQLSETASAQAQLSRFRIDAMDCPTEQTLIQDKLGKLAGI
EQLEFNLINRVLGVRHTLNGTAEIERAIDSLGMKAEPIAAEDDGTTSVAQPAKTRWWPLALSGIAAITAEIVHFAALAPE
WVVAGLALLAILGCGLGTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARNAIGG
LMQLAPDMATVQQADGLWRELDVREVAIGALVRVRPGERIGLDGEVTSGQSSVDQAPITGESLPVEKAVGDKLFAGTINQ
AGALEFRVTAAAGQSTLARIIKAVEEAQGARAPTQRFVDRFSRIYTPVVFAIALAVAVIPPLFMAGAWFDWVYRALVLLV
VACPCALVISTPVTIVSGLAAAARKGILIKGGVYLEGGRHLDFLALDKTGTITHGKPVQTDAEVLAPQFEARAQALAASL
GERSDHPVSRAIAQFGKAQGLALSEVDDFAALGGRGVRGTIDGEVYHLGNHRLVEELGLCSPALEAKLDALERQGKTVVL
LLDRSGPLALFAVADTVKDSSRQAIAELHELGIKTVMLTGDNPHTAQAIAAVVGIDRAEGNLLPADKLKTIEALYAEGHR
VGMVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILMQNIVLALGIKAIFLAITF
AGMATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 68
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 5e-111 48
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-112 42
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-112 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PputGB1_5192 YP_001671410.1 heavy metal translocating P-type ATPase BAC0298 Protein 0.0 67
PputGB1_5192 YP_001671410.1 heavy metal translocating P-type ATPase BAC0455 Protein 5e-80 42
PputGB1_5192 YP_001671410.1 heavy metal translocating P-type ATPase BAC0124 Protein 2e-66 41