Gene Information

Name : fliI (PputGB1_3927)
Accession : YP_001670153.1
Strain : Pseudomonas putida GB-1
Genome accession: NC_010322
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 4370289 - 4371644 bp
Length : 1356 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCACCTTGAGCGCACCAGTTTCGGCAAGCGCCTGGGCACCTACGCCGAGGCCATCGAACTGCCGGCGCAACCTGTCGT
TGAAGGCCGCCTGCTGCGCATGGTCGGCCTCACCCTGGAAGCCGAGGGCCTGCGCGCCGCAGTAGGCAGCCGTTGCCTGG
TGATCAACGACGACAGTTACCACCCGGTGCAGGTCGAGGCCGAAGTCATGGGCTTTTCCGGGCCCAAGGTGTTCCTCATG
CCGGTTGGCAGCATCGTCGGCATTGCCCCGGGCGCCCGGGTGGTGCCGCTGGATGACGGCGGCCGCCTGCCCATGGGCAT
GAGCATGCTGGGGCGGGTGCTCGACGGCGCCGGCCGCGCGCTGGATGGCAAGGGCGGCATGAAGGCCGAGGATTGGGTGC
CGATGGACGGCCCGGTGATCAACCCGCTCAACCGCGACCCTATCAGCAAGCCACTGGATGTGGGCATTCGCAGCATCAAC
GGTCTGCTTACTGTCGGCCGTGGTCAGCGATTGGGCCTGTTTGCCGGTACCGGTGTGGGTAAATCGGTGCTGCTGGGCAT
GATGACACGCTTCACTGAAGCCGAAATCATCGTGGTCGGCCTGATCGGTGAGCGGGGGCGCGAAGTGAAGGAATTCATCG
AGCATATCCTCGGTGAGGAGGGCCTCAAGCGCTCGGTGGTGGTGGCCTCGCCGGCTGACGATGCGCCCTTGATGCGCCTG
CGTGCCGCCATGTACTGCACGCGCATTGCCGAGTACTTCCGCGACAAAGGCAAGAATGTGCTGTTGCTGATGGACTCGCT
CACCCGTTTCGCCCAGGCTCAGCGGGAAATCGCCCTGGCCATCGGTGAGCCGCCGGCCACCCGGGGTTACCCGCCATCGG
TGTTCGCCAAGCTGCCAAAGCTGGTGGAGCGGGCAGGCAATGGCGAGCCGGGCGGTGGTTCGATCACGGCGTTCTATACC
GTGCTGTCCGAGGGCGACGACCAGCAGGACCCTATCGCCGACTCGGCACGGGGTGTGCTCGACGGCCACTTTGTGCTGTC
GCGCCGGCTGGCTGAGGAAGGCCATTACCCGGCTATCGACATCGAAGCCTCGATCAGCCGGGTCATGCCCCAGGTGGTCG
ATGCCGACCACTTGCGCCAGGCGCAGAAGTTCAAGCAGCTGTGGTCGCGCCTGTCGCAAAGCCGCGACCTGATCAGCGTG
GGTGCCTACGTGGCGGGTGGTGACCCGGAGACCGACCTGGCCATTGCCTTGCAATCGAAGCTGGTGGAGTTTCTGCGCCA
GGGCCTGCGTGAAAACGTGGGTATGGCGCAGAGCCGCGAACAGCTGGGGGCGATTTTCACGCCCCCGGCAGGCTGA

Protein sequence :
MHLERTSFGKRLGTYAEAIELPAQPVVEGRLLRMVGLTLEAEGLRAAVGSRCLVINDDSYHPVQVEAEVMGFSGPKVFLM
PVGSIVGIAPGARVVPLDDGGRLPMGMSMLGRVLDGAGRALDGKGGMKAEDWVPMDGPVINPLNRDPISKPLDVGIRSIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEAEIIVVGLIGERGREVKEFIEHILGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATRGYPPSVFAKLPKLVERAGNGEPGGGSITAFYT
VLSEGDDQQDPIADSARGVLDGHFVLSRRLAEEGHYPAIDIEASISRVMPQVVDADHLRQAQKFKQLWSRLSQSRDLISV
GAYVAGGDPETDLAIALQSKLVEFLRQGLRENVGMAQSREQLGAIFTPPAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-88 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-89 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-88 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-75 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-76 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-76 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-75 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-75 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-81 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-89 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-89 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-89 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-89 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 8e-82 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 4e-78 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-75 47
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-73 47
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-72 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-71 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-67 46
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-73 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 9e-72 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 6e-72 46
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 8e-73 46
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 4e-73 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-75 46
escN AAK26715.1 EscN Virulence LEE Protein 4e-74 46
escN AAL57542.1 EscN Virulence LEE Protein 4e-74 46
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-74 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-74 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-74 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-74 46
escN AAC38383.1 EscN Virulence LEE Protein 7e-75 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-75 46
escN AAC31513.1 L0034 Virulence LEE Protein 4e-75 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-75 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-70 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-70 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 9e-68 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 9e-68 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-71 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-71 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 5e-67 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-67 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001670153.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 87
fliI YP_001670153.1 flagellum-specific ATP synthase VFG2331 Protein 7e-122 62
fliI YP_001670153.1 flagellum-specific ATP synthase VFG2499 Protein 9e-125 62
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0515 Protein 6e-76 49
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0192 Protein 7e-88 48
fliI YP_001670153.1 flagellum-specific ATP synthase VFG2144 Protein 4e-86 47
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0390 Protein 3e-82 47
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0048 Protein 1e-85 46
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0730 Protein 4e-75 46
fliI YP_001670153.1 flagellum-specific ATP synthase VFG0812 Protein 3e-75 46