Gene Information

Name : PputGB1_1251 (PputGB1_1251)
Accession : YP_001667494.1
Strain : Pseudomonas putida GB-1
Genome accession: NC_010322
Putative virulence/resistance : Resistance
Product : D-isomer specific 2-hydroxyacid dehydrogenase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1052
EC number : -
Position : 1396180 - 1397169 bp
Length : 990 bp
Strand : -
Note : PFAM: D-isomer specific 2-hydroxyacid dehydrogenase catalytic region; D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; KEGG: pen:PSEEN1358 D-lactate dehydrogenase, NAD-dependent

DNA sequence :
ATGCGCGCCCTGTTGTTCAGCAGCCAGCACTACGACCAAGAAAGCTTCACCGAGGCTGCCAACGGCACGTCGCTGGAGCT
GCATTTCCAGCCCGCCCGCCTGACCCTGGACACCGCCGCACTGGCCGATGGTTTTGAGGTGGTGTGCGCCTTCATCAATG
ACGAACTCGACGCCACGGTGCTGCAGCGCCTGGCCGCAGGTGGCACACGGCTGATCGCCCTGCGCTCGGCCGGCTACAAC
CACGTAGACCTGGCTGCCGCCCAGCGCCTAGGCCTGGCCGTGGTACGCGTGCCGGCCTACTCGCCACACGCCGTGGCCGA
GCACGCCGTGGCGCTGATCCTGGCGCTCAACCGGCGCCTGCACAGAGCCTACAACCGTACCCGCGAAGGTGACTTCACCC
TGCACGGGCTAACCGGCTTCGACCTGCACGGCAAAACCGTGGGCGTGGTCGGCACCGGCCAGATCGGCGCCGCCTTCGCC
CGCATCATGGCAGGGTTCGGTTGCCAATTGCTGGCCTACGACCCCTACCCCAACCCAGAGCTGCTGGCCCTTGGCGCGCG
TTACCTGCCCCTGCCCGAGCTGCTGCGCGAAGCCCGCATCATCAGCCTGCACTGCCCGCTGACCGAACACACCCGCCACC
TGATCAATGCGCAAAGCCTGGCCCAGTTGCAGCCCGGCGCCATGCTGGTCAACACCGGTCGCGGCGCGCTGGTCGATACC
CCTGCGCTGATTGACGCGCTGAAAAGCGGCCAGCTTGGCTACCTGGGCCTGGACGTCTACGAAGAAGAAGCCCAGCTGTT
CTTCGAGGACCGCTCCGACCTGCCACTGCAGGACGATGTGCTGGCCCGCCTGCTGACCTTCCCCAACGTGATCATCACCG
CCCACCAGGCCTTCCTCACCCGCGAGGCGCTGGATGCCATTGCCCAGACCACGCTGGACAACATCAACCGCTGGGCGGCA
GGCAATCCACAGAATCTGGTGATGGGTTAG

Protein sequence :
MRALLFSSQHYDQESFTEAANGTSLELHFQPARLTLDTAALADGFEVVCAFINDELDATVLQRLAAGGTRLIALRSAGYN
HVDLAAAQRLGLAVVRVPAYSPHAVAEHAVALILALNRRLHRAYNRTREGDFTLHGLTGFDLHGKTVGVVGTGQIGAAFA
RIMAGFGCQLLAYDPYPNPELLALGARYLPLPELLREARIISLHCPLTEHTRHLINAQSLAQLQPGAMLVNTGRGALVDT
PALIDALKSGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIITAHQAFLTREALDAIAQTTLDNINRWAA
GNPQNLVMG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
EF2295 NP_815952.1 D-specific alpha-keto acid dehydrogenase Not tested Not named Protein 1e-42 42
vanHB YP_008394258.1 VanH protein, D-specific alpha-keto acid dehydrogenase Not tested Not named Protein 1e-42 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PputGB1_1251 YP_001667494.1 D-isomer specific 2-hydroxyacid dehydrogenase U35369.1.gene5.p01 Protein 8e-43 42
PputGB1_1251 YP_001667494.1 D-isomer specific 2-hydroxyacid dehydrogenase AF310956.2.orf4.gene Protein 2e-43 42
PputGB1_1251 YP_001667494.1 D-isomer specific 2-hydroxyacid dehydrogenase NC_005054.2598279.p0 Protein 3e-41 41
PputGB1_1251 YP_001667494.1 D-isomer specific 2-hydroxyacid dehydrogenase AF130997.1.orf3.gene Protein 5e-41 41
PputGB1_1251 YP_001667494.1 D-isomer specific 2-hydroxyacid dehydrogenase NC_014475.1.orf2.gen Protein 3e-41 41