Gene Information

Name : lysS (PputGB1_1101)
Accession : YP_001667345.1
Strain : Pseudomonas putida GB-1
Genome accession: NC_010322
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 1250740 - 1252242 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGCGACCTCAAGACCGAATCGCAAGACCTGCAACAGGAAGAAAATGCCCTGATCGCCCTGCGCAAGGAAAAACTTGC
CGCCGAGCGCGCCAAGGGCAACGCCTTCCCCAACGACTTCCGTCGCGACAGCTACTGCAACGACCTGCAGAAACAGTACG
CGGACAAGACCAAGGAAGAGCTGGAAGCAGCCGCGATCCCGGTCAAGGTTGCCGGCCGTATCATGCTCAACCGTGGCTCG
TTCATGGTTATCCAGGACATGACTGGCCGTATCCAGGTCTACGTCAACCGCAAAACCCTGCCCGAAGAAACCCTGGCTGC
GGTCAAGACTTGGGACCTGGGCGACATCATCAGTGCCGAAGGCACCCTGGCCCGTTCCGGCAAGGGCGACCTGTACGTCG
AAATGACCAATGTGCGCCTGTTGACCAAGTCGCTGCGCCCGCTGCCCGACAAGCACCACGGCCTGACCGACACCGAGCAG
CGCTACCGTCAGCGTTACGTCGACCTGATGGTCAACGAAGAAACCCGCCACACCTTCCGTGTGCGTTCGCAGGTGATCTC
GCACATCCGCAAGTTCCTCATCGAGCGTGACTTCCTCGAAGTCGAAACGCCGATGCTGCAGACCATTCCAGGCGGCGCGG
CGGCCAAGCCGTTCGAAACTCACCACAACGCCCTGGACATGGCCATGTTCCTGCGCATCGCGCCGGAGCTGTACCTCAAG
CGCCTGGTGGTGGGTGGCTTTGAAAAGGTGTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTTTCGACTCGTCACAA
CCCTGAATTCACCATGCTCGAGTTCTACCAGGCCTACGCTGACTACCGCGACAACATGGACCTCACCGAGGAACTGTTCC
GTGAGCTGGCGCAGCTGGTACTGGGCAGCACCGACGTGCCGTACGGTGACAAGGTGTTCCACTTTGGCGAGCCGTTCGTG
CGCCTGTCGGTGTTCGACTCGATCCTCAAGTACAACCCCGAGCTGACCGCTGCCGACCTGCAGGACGTCGACCGCGCCCG
CGACATCGCCAAGAAGGCCGGTGCCAAGGTGCTGGGCCACGAAGGCCTGGGCAAGCTGCAGGTGATGATTTTCGAAGAAC
TGGTCGAGCACAAGCTGGAGCAGCCGCACTTCATTACCGAGTACCCGTTCGAAGTGTCGCCGCTGGCCCGTCGCAACGAC
GACAACCCAGCCGTTACCGACCGCTTCGAGCTGTTCATCGGTGGCCGTGAAATCGCCAACGCCTACTCCGAGCTCAACGA
TGCCGAAGACCAGGCCGAGCGCTTCCTGGCCCAGGTGGCCGAGAAGGACGCCGGTGACGACGAAGCCATGCACTACGACG
CCGACTTCGTGCGTGCACTGGAGTACGGCATGCCGCCGACCGCCGGTGAGGGTATCGGCATCGACCGCCTGGTGATGCTG
CTGACCAACTCGCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGTCCGCAGGCCTGA

Protein sequence :
MSDLKTESQDLQQEENALIALRKEKLAAERAKGNAFPNDFRRDSYCNDLQKQYADKTKEELEAAAIPVKVAGRIMLNRGS
FMVIQDMTGRIQVYVNRKTLPEETLAAVKTWDLGDIISAEGTLARSGKGDLYVEMTNVRLLTKSLRPLPDKHHGLTDTEQ
RYRQRYVDLMVNEETRHTFRVRSQVISHIRKFLIERDFLEVETPMLQTIPGGAAAKPFETHHNALDMAMFLRIAPELYLK
RLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYRDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFV
RLSVFDSILKYNPELTAADLQDVDRARDIAKKAGAKVLGHEGLGKLQVMIFEELVEHKLEQPHFITEYPFEVSPLARRND
DNPAVTDRFELFIGGREIANAYSELNDAEDQAERFLAQVAEKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVML
LTNSPSIRDVILFPHMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-137 60
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 3e-78 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001667345.1 lysyl-tRNA synthetase VFG1668 Protein 1e-137 60