Gene Information

Name : lysS (Teth514_0810)
Accession : YP_001662449.1
Strain : Thermoanaerobacter sp. X514
Genome accession: NC_010320
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 842540 - 844039 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTAATTCAAATGATAACATTTGGAACAACACTGAAGAACTCAATGAACTTTTGCGAATAAGAAGAGAAAAATTAAA
TATTTTAAGAAGCATGGGAATAGAACCTTACGGTATTGACAGGTTTGAAAGGACTAATGTATCCTCTGATATTAAAAACG
ATTACGAGAATTTTGAAGGCAAAGTAGTGACTCTTGCAGGACGAATTATGTCCAAAAGGGCCCATGGCAAAGCTTCTTTT
GCAGATATTCAAGATAGAGATGGCAGGATACAGATTTACGTTAAGTATGATACAGTAGGAGAGAAGAATTATGAAATTTT
TAAAATCCTCGACATTGGAGATATAATAGGGGTTACAGGCGAGGTATTTAAATCTAAAACTGGTGAAATAACTATTCGCG
TAACAGACTTTAAGCTTTTGTCTAAATCTCTTCAAATACTTCCAGAGAAATGGCACGGTTTAAAAGACCCTGATTTGCGT
TATAGACAGAGATATACGGATTTAATAATAAATCCCGAAGTAAAAGAGGTGTTTTTAAAGAGGACGAAAATAATAAAGGC
GATAAGAGAATTTCTTGACAATAGAGGATTTTTAGAAGTGGAGACTCCTATTCTTCATACTATTGCGGGTGGGGCTGCAG
CAAGGCCTTTTATTACTCATCACAATGCTTTAGACATTGATATGTACTTGCGAATTGCTTTAGAGCTTCACTTAAAAAGA
CTTATTGTCGGTGGCCTGGAAAAAGTATATGAAATGGGCAGAGTTTTTAGAAATGAAGGAATGGATATAAGGCACAATCC
GGAATTTACTTTGTTGGAACTTTACGAAGCCTATACGGATTATTACGGTATGATGGAACTTACAGAACAATTATTTGCCT
ATGTGGCCCAAAAGGTTAACGGTACTACGAAAATAGTTTATCAAGGAACAGAAATAGATTTGACACCTCCTTGGAAGAGA
ATTACTATGGTGGATGCAATAAAAGAATATGTAGGTGTAGACTTTAATGAAGTTAAGGCAGATGCAGAAGCAGTAGAGAT
AGCGAAGAGATTAAACCTTGAGACAAAAGAAGGAATGAAAAAGGGAGAAGTAATAGCACTTGTTTTTGATGAGCTGGTAG
AACAACATTTGATACAGCCAACTTTTGTTATGGATTATCCAGTAGAGATTTCTCCTTTAGCTAAGAGAAAACACGACAAT
CCAGCATTTACTTCTAGATTTGAAGCCTTTATTTACGGAAGAGAAGTGGCTAATGCCTTTTCAGAATTAAATGACCCTAT
AGACCAAAAAGAAAGGTTTTTAGAACAATTAAAACAAAGAGAAGCCGGCGACGAAGAAGCTCATATGATGGATGAGGATT
TTATAAATGCTCTTGAGGTTGGTATGCCACCGACTGGAGGACTAGGTATAGGGGTAGACAGACTTGTGATGTTTATGACA
GATGCTTATTCTATAAGAGATGTAATATTATTCCCGACGATGAAGCCTAAAAATGACTAA

Protein sequence :
MSNSNDNIWNNTEELNELLRIRREKLNILRSMGIEPYGIDRFERTNVSSDIKNDYENFEGKVVTLAGRIMSKRAHGKASF
ADIQDRDGRIQIYVKYDTVGEKNYEIFKILDIGDIIGVTGEVFKSKTGEITIRVTDFKLLSKSLQILPEKWHGLKDPDLR
YRQRYTDLIINPEVKEVFLKRTKIIKAIREFLDNRGFLEVETPILHTIAGGAAARPFITHHNALDIDMYLRIALELHLKR
LIVGGLEKVYEMGRVFRNEGMDIRHNPEFTLLELYEAYTDYYGMMELTEQLFAYVAQKVNGTTKIVYQGTEIDLTPPWKR
ITMVDAIKEYVGVDFNEVKADAEAVEIAKRLNLETKEGMKKGEVIALVFDELVEQHLIQPTFVMDYPVEISPLAKRKHDN
PAFTSRFEAFIYGREVANAFSELNDPIDQKERFLEQLKQREAGDEEAHMMDEDFINALEVGMPPTGGLGIGVDRLVMFMT
DAYSIRDVILFPTMKPKND

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-112 50
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 6e-84 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001662449.1 lysyl-tRNA synthetase VFG1668 Protein 1e-112 50