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Name : topB2 (APJL_0746) Accession : YP_001651753.1 Strain : Actinobacillus pleuropneumoniae JL03 Genome accession: NC_010278 Putative virulence/resistance : Unknown Product : DNA topoisomerase III Function : - COG functional category : L : Replication, recombination and repair COG ID : COG0550 EC number : - Position : 813619 - 815535 bp Length : 1917 bp Strand : - Note : decatenates replicating daughter chromosomes DNA sequence : TTGAGAGTATTTTTAGCGGAAAAACCGAGTTTGGCAAGAGCAATCGCAGACGTATTGCCGAAGCCGCATACCCGTGGTGA CGGCTATATTCAATGTGGTGAAAATGATACGGTAACTTGGTGTATCGGTCATTTATTGGAACAAGCGGAACCTGATATTT ACGACGAACGTTTTAAAATGTGGCGAATGGAGCATTTGCCGATTGTCCCGCAGCAATGGAAATTAGTGCCAAAAAAAGAA ACGTTGAAGCAGTTAAAAGCGGTCGAAAAATTGGTAAAAAAAGCGGACGTATTGGTAAACGCCGGCGACCCGGACCGAGA AGGGCAGCTATTAGTTGATGAGGTCTTTAGCTATCTGAACGTACCGCCGGAAAAACGTAATCGGATTCAACGTTGTTTGG TGAGCGATCTCAACCCGAGTGCGGTAAAAAAAGCGATTGATAAATTGCAAAATAATCGTGATTTTATACCGCTTGCCACC TCTGCCTTAGCACGTGCAAGAGCCGATTGGCTATACGGCATTAATATGACGCGAGCTTATACCTTACAAGGACGTTGGGC GGGCTATCAGGGGGTATTGTCGGTGGGGCGTGTTCAAACCCCCGTGTTAGGTTTAATTGTGCGCCGCGATCTGGAAATAG AAAACTTTGTGCCGAAAGACTATTTTGAAGTATTGGCGCATATCGTTGTGCCGGAAACTCAAGAACGTTTTACTGCCCAA TGGCAACCGAGTAAGGCGTGCGAAGATTATCAAGATGAAGACGGTCGAGTATTATCTAAAAGTTTGGCGGAAAATGTCGT TAAACGCATTACCGACCAAGCAGCATTAGTAACGGATTATCAAGATAAAATTGAAACGGAAACCGCACCGCTGCCTTATT CATTATCGGTATTACAAATTGATGCGGCACGCCGTTACGGCCTATCGGCACAAGCTGTGTTGGATATTTGTCAGAAACTG TATGAAACGCATAAGCTGATTACCTATCCCCGTTCGGATAACCGTTATCTACCGAATGAGCATTACGCTGATCGTTTTAA AGTGATGGCGGCGATTGCACATCATCTGGATGAGTATAAAGAAAAACCGGAGGCGGTTAATCCGGAAATTCGTAATCGTT GTTGGAATGATAAAAAGGTAGAAGCGCACCATGCTATTATTCCGACCGTACGCCAAGGCTCGGTAAAACTAACTGAAAAC GAAAAGCGTATTTATCAACTGATTGCCCGTCAATACTTAATGCAATTTTGTCCGGATGCGGAATATCGCAAAGGTAAAAT TGTATTGGAGATTGCCGGAGGAACCTTTAACGCGCAGGCTCGGAACTTAATTGTTGCCGGTTGGAAAGCCTTATTAGGCA AAGAAGACAGTGACGAAGTGTTAGAGCCGTTATTACCGGTTGTGAAAAAAGGGCAGCAATTACATTGTGAAAAAGGTGAG ATTGTCAGTAAGAAAACCCAGCCGCCTCGTCATTTTACCGACGCAAGTTTATTATCGGCAATGACCGGTATCGCTCGTTT CGTACAAGATAAACAACTTAAAAAAGTCTTACGTGAAACGGACGGCTTAGGTACTGAAGCAACACGTGCCGGTATTATCG AATTGCTGTTTAAACGCGGATTTTTAATCAAAAAAGGACGAAATATCCACAGTACGGAAGCCGGGCGAATTCTAATTAGT GCCTTACCGGACGAAGCGACTCAGCCGGATATGACTGCGCATTGGGAAATGCAACTTACCGACATTAGTAAGAAGCAAGC GAGTTATCAGCAGTTTATGCAAGTACTTACGAGTAAATTACCTGAATTATTACATACGGAACGACAAAAATTGCAAAATT TAAGTCGAATCCAACCGCCCGCCTCTTATCGTAAGCGGTCAAATTTTAAGAAAAATTTACAAACTGCTGAAAAATAA Protein sequence : MRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDIYDERFKMWRMEHLPIVPQQWKLVPKKE TLKQLKAVEKLVKKADVLVNAGDPDREGQLLVDEVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNRDFIPLAT SALARARADWLYGINMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPETQERFTAQ WQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPYSLSVLQIDAARRYGLSAQAVLDICQKL YETHKLITYPRSDNRYLPNEHYADRFKVMAAIAHHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQGSVKLTEN EKRIYQLIARQYLMQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQLHCEKGE IVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGIIELLFKRGFLIKKGRNIHSTEAGRILIS ALPDEATQPDMTAHWEMQLTDISKKQASYQQFMQVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNLQTAEK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| topB | AAF14809.1 | topoisomerase B | Not tested | SPI-7 | Protein | 7e-109 | 41 |
| topB | NP_458624.1 | topoisomerase B | Not tested | SPI-7 | Protein | 1e-108 | 41 |
| topB | NP_807836.1 | DNA topoisomerase III | Not tested | SPI-7 | Protein | 1e-108 | 41 |
| pph56 | CAI36097.1 | Topoisomerase IA | Not tested | PPHGI-1 | Protein | 4e-103 | 41 |
