Gene Information

Name : lysS (BcerKBAB4_0072)
Accession : YP_001642969.1
Strain : Bacillus weihenstephanensis KBAB4
Genome accession: NC_010184
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80379 - 81878 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGCTTGTTCGTCGTGAAAAGCTACATAATTTACGTGAGCAAGG
AATCGATCCGTTCGGTAAACGATTTGAACGCACAAATTCAACAACTGACTTAGTAAGTCTATATGGAGAATTCTCTAAAG
AAGAATTAGAAGAGAAAGAAATCACTGTTTCTATCGCTGGTCGTATTATGACAAAACGCGGTAAAGGAAAAGCGGGATTT
GCGCATATTCAAGATTTACAGGGGCAAGTTCAAATTTATGTGCGTAAAGATACTGTTGGAGATGAAGAGTACGAGTTATT
TACGACAGCAGATTTAGGTGACTTAGTAGGTATTGAAGGTAAAGTGTTCAAAACAAACGTTGGAGAACTTTCAGTAAAAG
CAATAGGATTTACACTTTTAACGAAATCCCTTCGTCCATTACCGGATAAATACCATGGATTAAAAGATGTTGAACAACGC
TATCGTCAACGTTACTTGGACTTAATTACAAGTATGGAAAGCCGTGAAACGTTCGTTACCCGTAGTAAAATTATTCGTGA
AATGAGAAGATATTTAGATGACAACGGTTATCTTGAAGTGGAAACGCCTATGATGCACGCGATTGCAGGTGGAGCGTCTG
CTCGTCCTTTCACTACACACCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAACTTCATTTAAAACGT
CTTATTGTGGGTGGATTAGAAAAGGTTTATGAGATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAACCC
TGAGTTTACGATGATTGAATTATATGAAGCGTACGCTGATTATAATGATATTATGAAACTAACAGAAAATATGGTTGCTC
ATATCGCGAAAAAAGTATTGGGTACAACAACAATCCAATATGGTGATTATGAAATTAATCTAGAGCCAGAATGGACACGT
CTTCATATGGTAGATGCAATTAAGCAACACTCTGGAGCAGATTTCTGGAATCCAATGAGTGTAGAAGAAGCGCGTGAACT
TGCAAAAGAACATAATGTGGAAATTAAGAATACAATGGAAGTTGGTCATATTATTAATGAGTTCTTCGAACAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATTTCGCCACTTGCGAAAAAGAATGACGAAGAT
CCACGATTCACAGATCGTTTCGAGTTATTTATCGTTGCGCGTGAACATGCAAATGCATTCACTGAGTTAAATGATCCAAT
CGATCAAAAAGAACGTTTTGAAGCTCAACTAAAAGAGCGCGAGCAAGGTAATGACGAAGCTCACATGATGGATGATGATT
ATATCGAAGCTCTTGAGTACGGTATGCCTCCTACGGGTGGGTTAGGAATCGGTATTGATCGTCTTGTTATGTTATTAACA
AACGCACCATCCATTCGTGACGTACTATTATTCCCAGCTATGCGCCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEITVSIAGRIMTKRGKGKAGF
AHIQDLQGQVQIYVRKDTVGDEEYELFTTADLGDLVGIEGKVFKTNVGELSVKAIGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYNDIMKLTENMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQHSGADFWNPMSVEEARELAKEHNVEIKNTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-121 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001642969.1 lysyl-tRNA synthetase VFG1668 Protein 6e-122 50