Gene Information

Name : BcerKBAB4_2204 (BcerKBAB4_2204)
Accession : YP_001645052.1
Strain : Bacillus weihenstephanensis KBAB4
Genome accession: NC_010184
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 2270779 - 2273280 bp
Length : 2502 bp
Strand : -
Note : TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; heavy metal translocating P-type ATPase; PFAM: Haloacid dehalogenase domain protein hydrolase; Heavy metal transport/detoxification protein; E1-E2

DNA sequence :
ATGACAGATAAAAAGGAGAGCGAAAAACAAACAAATTGCTGTGAAAATTCTTCCATAGCATCCACAAAAGAAGAATTAGC
AACGGAAACTGCATCTTGTTGCTCTTCCAAAAAAGAGATTCCTATTGTGCCAATTGTTAAAAACACTTCTTGTTGTAATG
GTGAAACCAAAACTGAAAAAGAAAAAACTACAAAAGAAACATCGTCATGTTGCTCTTCTAAAACAGAGATTCCAATTATC
CCTATTACTAAGGAAACCTCTTGCTGTAGTAGTAACAACAATTCTATTAAAACTGAAACTTGTTGTTCAGCTGAATTAGC
CCTACAAAAAGAATCAATGACATCAAAATCTTCTTGCTGTAGTGATAGCTCATGTGAAGATCCATCACCTCTTCTTAATA
CTCAGAAAATATCAGGAGATGGCGTACAAACTTATCTTGTAGAAGGTATGGATTGCGGTGCATGCGCTTTAACAATCGAA
AAGCATCTACACAACGTTTCTGGCGTAGAGGAAGTTCGAGTAAACTTCGCTACTGGAAAAATGCATATTCGTCATGACCG
AAATGTGGATGACATTATAAAAGAAGTATCTAACGCAGGCTTTGAAGCCACTTTAGCTGGAGCTCGTAGAGGATCAACAC
CTGTTTCAAAATCAAAAAATACAACTTTGATTCTCTCAGGATTATTTTTAGCTTTAGGATTTGGAGGAAACTTTACAAAC
ATATCCCCTCTTCTTATTACACTTCTCTATGCGGTGAGCATTGGTATTAGTGGTTATAAACCTGCTAAAAGTGCCTTTTA
TGCAATTAGAAGTAAATCACTCGATATGAACGTTCTCATGATATCAGCCGCTATTGGTGCTGCATTAATTGGTCAATGGC
TAGAAGGCGCAACAGTTGTTTGGCTATTTGCATTAGGGGCAACGCTACAAAATAAATCTATTGAACGCACAAGAGAATCT
ATTCGTGGATTAATTGATTTAGCCCCATCAGAAGCTTGGGTAAAAATTGGAACTGAACTTATAAAAAAATCTGTTGATGA
TATTGCAGTGAATACAACGATTGTTGTAAAGCCAGGTGAAAAAATCCCATTAGACGGCACAGTTATAGGAGGTACTTCTA
CTGTAAACCAAGCGCCAATAACTGGTGAATCTATCCCAATCGATAAACAAATTGGCGATTCCGTATATGCAGGAACAATC
AATGAAGAAGGTTCGATTGAGATTACAGTAACTAAACTTGTTGAGGATACTACATTATCTCGAATTATTCATCTTGTAGA
GGAAGCACAGGAAAATAAAGCACCGACAGAAGCATTTGTAGATCGTTTCGCAAAAATCTATACACCTATCGTTTTTGTAT
TAGCAATTGGTGTCATGATTATCCCACCCCTTCTTGGTATGGGCGAATGGATGGAATGGATTTATAAAGGTTTAGAACTT
CTTGTTGTTGCGTGTCCTTGCGCTTTAGTTATCTCAACTCCTGTTGCTATTGTATCTGCAATTGGAAATGCAGCAAGAAA
CGGTGTACTCATTAAAGGTGGTACAGCTTTAGAAATTGCAGGTTCTTTAAATGCTATTGCGTTTGATAAAACAGGAACAT
TGACTGAAGGAAAACCAAAAGTAATGCATGTACGAAGCGTAGATTGTACTGAAGATGAGTTATTATCCATCGCAGCAACA
ATTGAAGAATACTCTAATCATCCCATTGCAAAAGCCATTACAGCATATGCGAAAGAACACCAAACTTCTATTCAAAGTGG
AACCGACTTGCGCGCCATTGTAGGAAAAGGTGCGCAAGTCACGATAGATGGCGAAACATACTACGCTGGAAATAAAGTAT
TATATGAAGATTTTGGAGTTTCGCTTCAAATGTGGAACGAACCAATTCAGGAAATGCAAAGAATTGGTCAAACCGTGATA
CTTGTCGGAACGAACAAAGTCATTTTAGGTATGATTTCAGTAGCAGATTCCATTCGTTCTACTACTTATGGAACAATTCA
AGAATTAAAACGATCAGGTATTCAGGAAACTATTATGTTAACAGGAGACAATGAAGGAACAGCTGAACATATTGCACAGA
AAGCAAAAATAGATCGTTATTTTGCTAACTTACTTCCAGAAGATAAAGTTCATTCTGTCAAACAACTTCAATCTGAAGGA
AAAACAGTGGCTATGATTGGGGACGGCATAAATGATGCCCCTGCCCTCGCTACTGCAAACCTAGGGATTGCAATGGGCGG
TGCAGGAACTGATACCGCAATGGAAACGGCAGATATTGTATTAATGGCAGATAATCTTGAAAAACTTCCTTATACAATGA
AACTAAGTCGGAAAGCATTGCACATCATAAAACAAAATATTTGGTTCTCATTGATTATTAAATTTATCGCCCTTGCCTTT
ATCTTCCCAGGTTGGCTCACTCTTTGGATGGCTGTCCTAAGTGATACAGGCGCTGCACTTATTGTAATCCTTAACAGTAT
GCGTTTATTACGGAACAAATAA

Protein sequence :
MTDKKESEKQTNCCENSSIASTKEELATETASCCSSKKEIPIVPIVKNTSCCNGETKTEKEKTTKETSSCCSSKTEIPII
PITKETSCCSSNNNSIKTETCCSAELALQKESMTSKSSCCSDSSCEDPSPLLNTQKISGDGVQTYLVEGMDCGACALTIE
KHLHNVSGVEEVRVNFATGKMHIRHDRNVDDIIKEVSNAGFEATLAGARRGSTPVSKSKNTTLILSGLFLALGFGGNFTN
ISPLLITLLYAVSIGISGYKPAKSAFYAIRSKSLDMNVLMISAAIGAALIGQWLEGATVVWLFALGATLQNKSIERTRES
IRGLIDLAPSEAWVKIGTELIKKSVDDIAVNTTIVVKPGEKIPLDGTVIGGTSTVNQAPITGESIPIDKQIGDSVYAGTI
NEEGSIEITVTKLVEDTTLSRIIHLVEEAQENKAPTEAFVDRFAKIYTPIVFVLAIGVMIIPPLLGMGEWMEWIYKGLEL
LVVACPCALVISTPVAIVSAIGNAARNGVLIKGGTALEIAGSLNAIAFDKTGTLTEGKPKVMHVRSVDCTEDELLSIAAT
IEEYSNHPIAKAITAYAKEHQTSIQSGTDLRAIVGKGAQVTIDGETYYAGNKVLYEDFGVSLQMWNEPIQEMQRIGQTVI
LVGTNKVILGMISVADSIRSTTYGTIQELKRSGIQETIMLTGDNEGTAEHIAQKAKIDRYFANLLPEDKVHSVKQLQSEG
KTVAMIGDGINDAPALATANLGIAMGGAGTDTAMETADIVLMADNLEKLPYTMKLSRKALHIIKQNIWFSLIIKFIALAF
IFPGWLTLWMAVLSDTGAALIVILNSMRLLRNK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 7e-147 47
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 6e-147 47
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 3e-143 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BcerKBAB4_2204 YP_001645052.1 heavy metal translocating P-type ATPase BAC0092 Protein 6e-95 42
BcerKBAB4_2204 YP_001645052.1 heavy metal translocating P-type ATPase BAC0298 Protein 1e-102 42