Gene Information

Name : BcerKBAB4_2177 (BcerKBAB4_2177)
Accession : YP_001645026.1
Strain : Bacillus weihenstephanensis KBAB4
Genome accession: NC_010184
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2237069 - 2238685 bp
Length : 1617 bp
Strand : +
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase; KEGG: bca:BCE_2400 2,3-dihydroxybenzoate-AMP ligase

DNA sequence : 
ATGTTAATAGGTTATACGGAATGGCCAAAAGAATTTGCCAATCGCTATCGAGAAGAAGGGTGTTGGCTTGGAGAAACATT
TGGTTCGATGTTAAAAGAACGCGCTGAAAAACATGGAGATCAAATTGCGATTGTAAGTGGTAATTCACAAATAACATATA
GTGAACTTGATAAACAGGTAGATCGCTTAGCTTCAGGTTTACTGAATTTAGGAATAAAGAAAGAGGATCGAGTTGTAATC
CAGTTACCTAATATTATAGAGTTTTTCGAAATATGCTTTGCACTATTTCGAATTGGAGCGCTTCCTGTTTTTGCACTACC
TTCACATCGAAGCAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCTTACGTTATTTCAGACAAGGCTCTCG
GCTTTGATTATCGAAAACTAGCAAGAGAAGTAAAAGTGAAAGTGCCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAGTTTGTGAACATAAGTGGTCTGTATATAGATTCCGTTCCATTACCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGAGGGACGACAGGACTTTCTAAATTAATTCCTAGAACACATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGACATTTGTAATTTAAATGCAGAAAGCGTCTATATGGCAGTTCTTCCAGTAGCACACAATTACCCGATGAGTTCT
CCAGGAACATTTGGAACTTTCTATGCGGGTGGAAAAGTAGTATTGGCAACTGGAGGTAGTCCAGATGAGTCATTTGCTCT
TATAGAAAAAGAAAAAGTTACGATTACAGCGCTCGTTCCACCATTAGCAATGATTTGGCTTGATGCTGCATCTTCCCGTA
ATAACGATTTATCAAGCCTGCAAGTTATTCAAGTAGGCGGTGCGAAATTTAGTGCCGAAGTTGCGAAGCGTATACGCCCT
ACATTCGGATGTACGTTACAGCAAGTATTTGGTATGGCGGAAGGCTTAGTAAATTACACAAGACTAGATGATCCAGAAGA
AATCATTATTCATACACAAGGTAGACCGATGTCTACATTTGATGAAGTTCGAGTTGTTGATGAACATGATAATGATGTTA
AGCCTGGTGAAGTAGGTAGTTTATTAACAAGAGGGCCATATACAATTCGTGGCTATTATAAAGCGGAAGAGCATAATGTA
AGATCATTTACAGAAGATGGTTTTTATCGCACAGGTGATCTTGTAAAAGTAAATGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAACCGTGGTGGTGAGAAGGTTGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCAATTGTATCTATGCCTGACGATTATTTAGGAGAACGCACTTGCGCTTTTGTTATCGCTCGTGGA
CAAGTCCTAACTGCAAACGAATTAAAAATGTTCTTAAGAGAACGTGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCACAAACAGGTGTTGGAAAAGTAAGCAAAAAAGAATTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLIGYTEWPKEFANRYREEGCWLGETFGSMLKERAEKHGDQIAIVSGNSQITYSELDKQVDRLASGLLNLGIKKEDRVVI
QLPNIIEFFEICFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKVKVPTLQHVIVVGEE
EEFVNISGLYIDSVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSADICNLNAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDESFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLQVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSTFDEVRVVDEHDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNV
RSFTEDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QVLTANELKMFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-123 49
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-123 49
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-123 49
ybtE CAA21394.1 - Virulence HPI Protein 1e-123 49
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-123 49
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-123 49
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 8e-124 49
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-123 49
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-123 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BcerKBAB4_2177 YP_001645026.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 9e-143 52
BcerKBAB4_2177 YP_001645026.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 1e-117 48
BcerKBAB4_2177 YP_001645026.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 5e-116 44