Gene Information

Name : fliI (Mext_0823)
Accession : YP_001638299.1
Strain : Methylobacterium extorquens PA1
Genome accession: NC_010172
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 896751 - 898106 bp
Length : 1356 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGACGCAGGAGCGCGGCTCCGGGGGGCTGGCGGCGGCACTCGCCGCGCTCTCGACCGTCGAGACCCTGGAGACCTTCGG
CCGGGTGACGGCGATCCGCGGCCTTCTGGTCGAGGTGGCCGGGCCGGTTTCCGCGATGCGGCTCGGCGGTCGCATCGACG
TGGTCGTGGAGGGCGCCGGGGTCGCGGCCTCGACGGTGCCGTGCGAGGTGATCGGCTTTGCCGGCGACCGGGCGCTCGCC
ATGCCGTTCGGCTCGCTGGAGGGGGTGCGCCGCGGCTGTCCGGCCCTGGTGCGTGACGAGGCGGCGGGCGCGATCCGCCC
CTCCGCCGCTTGGCTCGGGCGCACCGTCGATGCCCTCGGGCGCCCGATCGACGGCCTCGGCCCCCTCGCGCAGGGGCCCG
CGATCTATCCCCTGCGGGCCGACCCGCCCCCCGCGCACGGACGACGGCGCGTCGGGCCGCCGCTCGATCTCGGGGTTCGC
TGTATCAACACCTTTCTCACCATGTGCCGCGGGCAGCGCATGGGCATCTTCGCCGGCTCCGGCGTCGGCAAGTCGGTGCT
GCTCTCGATGCTGGCCCGCTACACCGCCGCCGACGTGGCGGTGATCGGCCTCGTCGGCGAGCGCGGCCGCGAGGTTCAGG
AGTTTCTCCAGGACGACCTCGGCGCGGCGGGGCTCGCCCGCTCGGTCGTAGTGGTGGCGACCTCCGACGAGCCGGTGCTG
ATGCGCCGCAACGCCGCCTACGTGACCCTGGCGGTGGCCGAGTACTTTCGCGACCAGGGCGCGCAGGTCTTGTGCATGAT
CGATTCGATCACCCGCTTCGCCATGGCCCAACGCGACATCGGACTCGCTGGCGGCGAGCCCCCGACCGCCAAGGGCTACA
CGCCCACCGTCTTCTCCGAATTACCGCGCCTGCTCGAACGGGCCGGGCCGGGGGTAGGGGAGGGGGCGATCTCCGGCCTG
TTCACCGTGCTGGTGGAGGGCGACGACCACAACGAGCCGGTGGCGGATGCGGTGCGCGGCATCCTCGACGGGCACATCGT
TATGGAGCGGCGCATTGCCGAACAGGGGCGCTACCCGGCGATCAACGTGCTGCGCTCGGTCTCGCGCACCATGCCGCGGG
CCTGCGACCCGGCCCGTCTCCCCACCGTCCGCCGTGCGCGAAAGGTGCTGGCGACCTATGCCGACATGGAGGAGCTGATC
CGGCTCGGGGCCTACCGTGCCGGTTCCTCGCCGGAGGTGGACGAAGCGGTGGCTCTCATGCCCGATCTGACGGCTTTTCT
GGGGCAGGGTAAGGAAGAAGCAACCTCCATCAGCGAGGGTTATGACCGGTTGGCCGCCATTGTCGGCGGTGCGTGA

Protein sequence :
MTQERGSGGLAAALAALSTVETLETFGRVTAIRGLLVEVAGPVSAMRLGGRIDVVVEGAGVAASTVPCEVIGFAGDRALA
MPFGSLEGVRRGCPALVRDEAAGAIRPSAAWLGRTVDALGRPIDGLGPLAQGPAIYPLRADPPPAHGRRRVGPPLDLGVR
CINTFLTMCRGQRMGIFAGSGVGKSVLLSMLARYTAADVAVIGLVGERGREVQEFLQDDLGAAGLARSVVVVATSDEPVL
MRRNAAYVTLAVAEYFRDQGAQVLCMIDSITRFAMAQRDIGLAGGEPPTAKGYTPTVFSELPRLLERAGPGVGEGAISGL
FTVLVEGDDHNEPVADAVRGILDGHIVMERRIAEQGRYPAINVLRSVSRTMPRACDPARLPTVRRARKVLATYADMEELI
RLGAYRAGSSPEVDEAVALMPDLTAFLGQGKEEATSISEGYDRLAAIVGGA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-78 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-78 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-80 46
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-78 46
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-78 46
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-78 46
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-70 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-76 45
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-77 45
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-77 45
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-76 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-65 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-65 45
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-65 45
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-65 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-65 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-68 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-68 45
escN AAC31513.1 L0034 Virulence LEE Protein 1e-68 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-68 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-68 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-68 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-69 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-68 44
escN AAC38383.1 EscN Virulence LEE Protein 5e-69 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-69 44
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-68 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-57 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-57 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-57 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-57 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-68 43
escN AAK26715.1 EscN Virulence LEE Protein 2e-68 43
escN AAL57542.1 EscN Virulence LEE Protein 2e-68 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-61 42
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-62 42
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-62 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-66 42
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-59 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-61 41
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-69 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-65 41
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-66 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001638299.1 flagellum-specific ATP synthase VFG2499 Protein 5e-85 51
fliI YP_001638299.1 flagellum-specific ATP synthase VFG2144 Protein 1e-83 48
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0048 Protein 8e-82 48
fliI YP_001638299.1 flagellum-specific ATP synthase VFG1254 Protein 6e-86 47
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0390 Protein 3e-81 47
fliI YP_001638299.1 flagellum-specific ATP synthase VFG2331 Protein 1e-81 46
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0192 Protein 1e-80 46
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0515 Protein 1e-65 45
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0730 Protein 3e-69 44
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0812 Protein 5e-69 44
fliI YP_001638299.1 flagellum-specific ATP synthase VFG0555 Protein 1e-57 44
fliI YP_001638299.1 flagellum-specific ATP synthase VFG2459 Protein 4e-61 41