Gene Information

Name : fliI (Mext_0682)
Accession : YP_001638165.1
Strain : Methylobacterium extorquens PA1
Genome accession: NC_010172
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 743780 - 745108 bp
Length : 1329 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAGCCTCAACGCGATCGAGCGCCTGGAACGGGCGATGGAGGCCGGGCGCCGCGACCTGCCGCTGGTGCGGGTCGCCGG
CCCCGTGCGCGAGGTGACCGCCAGCGCGGCACGGGTCGCGGGCGTGTCCGCCTTCGTGCGGCTCGGCGACCGCATGGGTT
TTTCCGCCGACGGCCGCACGCAGGTCGGCGAGGTGGTCCGCATCGATGCGGCCGGCGCTACGGTGAAGCCGTTCGAGAGC
CGGATCGAGGCCGGCATCGGCAGCCTCGCCCACCGCATCGGCCCGGCGCAGCTGCGCCCGGACCCACGCTGGAAGGGCCG
GGTGATCGACGCGCTCGGCCGCCCCGTCGATGGGCTCGGCCCCCTGGCCGAGGGGCCCGCCAGCGTTCCGCTCGATGCCG
ATCCGCCCGCCGCGATGACCCGCGCGCGGGTGAAGACGCCGCTGCCCACGGGGGTGCGGGCGATCGACCTGTTCACGCCG
CTCTGCGCCGGCCAGCGCATCGGCATCTTCGCCGGCTCCGGCGTCGGCAAATCGACCTTGCTGGCGATGCTGGCCGGCGC
CCAGGGCTTCGACAGCGTGGTGGTCGCGCTGGTGGGCGAGCGCGGACGTGAGGTGCGCGAGTTCCTCGAAGGCCCCATCG
CCGCCGCCCGCGACCGCGCCGTGATCGTGGTCTCGACCGGTGACGAGAGCCCGATGATGCGCCGGCAAGCGCCGAAGGTG
GCGCTGGCCGTGGCCGAGTCCTTCCGCGACCGCGGCGAGTCGGTGCTGCTCATCGTCGATTCCGTGACCCGCTACGCCCA
CGCCGCCCGCGACGTGGCGCTCGCCGCCGGTGAGCCGGCGGTGGCGCGCGGCTACCCGCCGAGCGTCTTTTCGGACTTGC
CGCGGCTGTTGGAGCGCGCCGGCCCCGGCGCGGAGGGCACCGGCACGATCACCGGCATCTTCTCGGTGCTGGTCGACGGC
GACGACCACAACGACCCGGTCGCCGACTCGATCCGCGGCACCCTCGACGGCCACGTGGTGCTCGACCGCGCCATCGCCGA
GCAAGGCCGCTACCCGGCGATCGAGCTGCTTGGCTCGATCTCGCGCCTGGCGGGTGAGGTCTGGACCGGAGACCAGCGCG
AGTTGGTGCGCAAGCTCAAGAGCATGATGGCCCGCTTCGAGGAGACACGGGATCTGCGCCTGATGGGCGGCTACCGCGCC
GGCAGCGATCCCGACCTCGACCAAGCCGTCAGCCTCGTGCCGCGGATCTACGAGGCCCTGCGGCAGGAGCCGGGCCAGCC
GCCGAGCCGCGACGCCTTCCTCGAACTCGCCCAATCCCTGCGGGGATGA

Protein sequence :
MSLNAIERLERAMEAGRRDLPLVRVAGPVREVTASAARVAGVSAFVRLGDRMGFSADGRTQVGEVVRIDAAGATVKPFES
RIEAGIGSLAHRIGPAQLRPDPRWKGRVIDALGRPVDGLGPLAEGPASVPLDADPPAAMTRARVKTPLPTGVRAIDLFTP
LCAGQRIGIFAGSGVGKSTLLAMLAGAQGFDSVVVALVGERGREVREFLEGPIAAARDRAVIVVSTGDESPMMRRQAPKV
ALAVAESFRDRGESVLLIVDSVTRYAHAARDVALAAGEPAVARGYPPSVFSDLPRLLERAGPGAEGTGTITGIFSVLVDG
DDHNDPVADSIRGTLDGHVVLDRAIAEQGRYPAIELLGSISRLAGEVWTGDQRELVRKLKSMMARFEETRDLRLMGGYRA
GSDPDLDQAVSLVPRIYEALRQEPGQPPSRDAFLELAQSLRG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-58 46
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-53 46
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-53 46
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-53 46
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-53 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-60 45
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-60 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 7e-61 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-61 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-60 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 6e-63 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 7e-53 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 7e-53 44
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-63 43
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-64 43
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-64 43
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 43
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-62 43
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 43
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-52 43
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-57 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-57 43
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-56 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 9e-52 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-50 43
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-62 42
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-62 42
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-62 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 5e-52 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-48 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001638165.1 flagellum-specific ATP synthase VFG0192 Protein 7e-59 48
fliI YP_001638165.1 flagellum-specific ATP synthase VFG2331 Protein 1e-65 48
fliI YP_001638165.1 flagellum-specific ATP synthase VFG2144 Protein 6e-64 47
fliI YP_001638165.1 flagellum-specific ATP synthase VFG0555 Protein 1e-53 46
fliI YP_001638165.1 flagellum-specific ATP synthase VFG0515 Protein 1e-60 45
fliI YP_001638165.1 flagellum-specific ATP synthase VFG1254 Protein 3e-62 44
fliI YP_001638165.1 flagellum-specific ATP synthase VFG0048 Protein 2e-64 41
fliI YP_001638165.1 flagellum-specific ATP synthase VFG1008 Protein 7e-49 41