Gene Information

Name : lysS (lhv_0299)
Accession : YP_001576817.1
Strain : Lactobacillus helveticus DPC 4571
Genome accession: NC_010080
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 305148 - 306683 bp
Length : 1536 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAATGACCAATTACGTGTTCGTCGTCAAAAGATGGACGAAATGCGTGAAAACGGAATTGAACCATTCGGTCAAAGAAA
GTTTGATCGTCAAGATTTGGCAAGTACTTTGGAAGAAAAGTATGGTAAAGAAGATGAAGACGAATTAAACGATGATATGC
CTAAGACTAAGATTGCAGGTCGTATGCTTGCAAAACGTGGTAAGGGTAAAGTAGGCTTTGCAGACCTTTACGACCGTACT
GGTAAGATTCAAATTTATGTACGTAAAGACATCGTAGGCGAAGACAACTACAAGATCTTCAAGAAGTCAGATATTGGTGA
TTTCTTGGGTATTGATGGGGAAGTTATGAAAACCGATACTGGTGAGTTGACTATTCGTGCTACTCACGTAACCTTCTTAT
CTAAGGCTCTTCGTCCTTTGCCAGACAAGTACCACGGCTTGAAGGATGTTGAAGCAATCTACCGTCAACGTTACCTTGAC
TTGATTACTAATCGCGATAGCTACATGCGTTTTGTTCACCGTACTCAAATTATCCAAGCTATTCGTGATTACTTGAATAA
GCAAAACTTCTTGGAAGTTGAAACTCCAGTATTGCACAACATTCCAGGTGGTGCTGAAGCTCGTCCATTTATTACTCACC
ACAACGCTTTGGATATTGAACTTTACATGAGAATTGCTTTGGAACTTCCACTGAAGCGTTTGATTGTTGGTGATATGGAA
CGTGTTTACGAAATCGGTCGTGTATTCAGAAACGAAGGTTTGGACACTCACCACAACCCAGAATTTACTGAACTTGAAAG
TTATGCTGCATACTGGGACTTCCACGATGTTATGGACGAAGCAGAAGGCATTATTCGTGCTGCAGCTAAGGTTGTTTCAG
ATGATGGCAAGATTACTTACCAAGGTCAAGACATTGACTTAGGCAAGCCATTCCGTCGCATTCACATGGTTGATTTAATC
AAGGAAAAGACCGGCGTTGATTTCTGGAAGCCAATGACTCTTGAAGAAGCAACTAAGATTGCTGAAGATCACGATATTCA
TGTTGAAAAGTTCTGGAAGGTTGGCCACATTATTAACGCCTTCTTTGAAAAGTACTGTGAAGAAACTATTGTTGACCCAA
CATTTGTTTACGGTCACCCAGTAGAAATTTCACCACTTGCTAAGAAGAATGCTGACGATCCAAGATTTACTGACCGTTTC
GAAATTTACATCATGGGTGAAGAATACGGTAACGCCTTCTCAGAATTGAACGATCCTGATGATCAACGTGCTCGTTTCGA
AGCTCAAATGGAAGAACGTGAAGCAGGTAACGATGAAGCTGATATGATTGATGAAGATTACCTTCGTGCTATGGAATACG
GTATGCCACCTACAGGTGGTTTGGGTATCGGCATTGACCGTTTGGTAATGCTTTTGACTGATGCTCCAGCTATTCGTGAT
GTTTTGCTCTTCCCAACAATGCGTCCAGAACGTGCTGAATCAATTGATGCCGAAGTTAAGGCTGATTTAGCTAAGAAGAA
TAAAGAAAACAAGTAG

Protein sequence :
MNDQLRVRRQKMDEMRENGIEPFGQRKFDRQDLASTLEEKYGKEDEDELNDDMPKTKIAGRMLAKRGKGKVGFADLYDRT
GKIQIYVRKDIVGEDNYKIFKKSDIGDFLGIDGEVMKTDTGELTIRATHVTFLSKALRPLPDKYHGLKDVEAIYRQRYLD
LITNRDSYMRFVHRTQIIQAIRDYLNKQNFLEVETPVLHNIPGGAEARPFITHHNALDIELYMRIALELPLKRLIVGDME
RVYEIGRVFRNEGLDTHHNPEFTELESYAAYWDFHDVMDEAEGIIRAAAKVVSDDGKITYQGQDIDLGKPFRRIHMVDLI
KEKTGVDFWKPMTLEEATKIAEDHDIHVEKFWKVGHIINAFFEKYCEETIVDPTFVYGHPVEISPLAKKNADDPRFTDRF
EIYIMGEEYGNAFSELNDPDDQRARFEAQMEEREAGNDEADMIDEDYLRAMEYGMPPTGGLGIGIDRLVMLLTDAPAIRD
VLLFPTMRPERAESIDAEVKADLAKKNKENK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-109 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001576817.1 lysyl-tRNA synthetase VFG1668 Protein 1e-109 47