Gene Information

Name : Sare_4933 (Sare_4933)
Accession : YP_001539671.1
Strain : Salinispora arenicola CNS-205
Genome accession: NC_009953
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase and ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 5599143 - 5600810 bp
Length : 1668 bp
Strand : +
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: tfu:Tfu_1871 2,3-dihydroxybenzoate-AMP ligase

DNA sequence : 
ATGCCGCCGACCGCGGTTCACCCGACCCGTACGGGAACCGTTCCCTGGCCCGCCGAGGTAGCCACGCGCTACCTCGCCGA
GGGTTACTGGGCGGGCCGACCCCTGGGGGCGTACCTCACCGCTGCCGCTCGCGCGAACCCGGCCGCGATCGCACTGGTCG
ACGGCGATCTGCGGCTGAGCTACCGGGAGCTGATGTCCCGCGCCGACGGCGCTGCGGCCCGGCTCGTCGAGCGTGGCGTC
AGCGGCGACGACCGGGTCGTGGTGCAGTTGCCGAACTGCTGGGAGCACATCGTGCTCACCGTGGCCTGCCTGCGACTCGG
CGCCGTCCCGGTGTGGGCTCTGCCGGAGCACCGACTGCGCGAAATCACCGGCGTCGCCGCCCGCGCCGAGGCCCGGGTAC
TGGTAGTACCCGCCCGGCATCGCGAGTTCGACCACAGGGCGATGGCGCACGAGGTGGCCGCCACCGTACCCAGCATCGAG
CACGTCCTGGTGACCGGATCCGCAGATCCAGGTGAGGATCTGGGCAGGCTGTGTGAGCCGGCAGCAGACCCCGCCGCGCT
GTCCGCACGCTTCGACGCGGCTGCGCCGGATGCTACCGCGGTGGCGACGTTCCTGCTCTCCGGCGGCACCACCGGCACGC
CCAAACTCGTGCCCCGAACGCACAACGACCTCGCCTACATGGTCGGCGAGGCAGCCCGACTGTGCGAGTTCGGTCCGGAC
ACGGCGTATCTCGCGGCTCTTCCGCTCGGCCACGGATTCCCGTACACCGGTCCGGGTGTTCTGGGCGCGCTGATGTCCGG
CGGCCGGGTGGTCATCGCCGCCTCCCCCGCCCCCGGGCCGGCGTTGGCGACGATCGAACGCGAGCGGGTCACCGCGACGT
CGATCGTCCCGGCGATCGCGCTTCGTTGGTTGGCGCACCACGCGGCCCACCCCGGCCGGGACCTGGGTTCCCTGCGTCTG
GTGCAGATAGGGGCGGCACGTCTGGAGCCCGACGCCGCGGCCCGGATCGAGCCCGAGCTGGGGGGACGGCTGCAGCAGGT
GTTCGGGATGGGGGAGGGTCTGCTCTGCCTGACCCGCCTGGACGATCCGCCGGCAGTCGTGCACCACACCCAGGGCCGGC
CGATCAGCCCCGCCGACGAGGTTCTCATCGTCGACGACGAGGACCAGCCGGTGCGGCCGGGGGAGGCGGGGGCGCTACTC
ACCCGCGGCCCGTACACCCTTCGCGGCTACTACCGCTCGCCCGAGATAGACGCGGCGTCCTTCCTGGCTGATGGTTGGTA
CCGAACCGGCGACATCGTCCGCCAGACGCCGGACGGGAACCTGGTGGTCACCGGCCGCGAGAAAGATCTGATCAACCGTG
GTGGTGAGAAGGTCAGCGCCGTCGAGGTCGAGGGTTTCGCGCTCGCTCTCGACGGGGTCACCCAGGCAGCCGCCATGGCG
ATGTCGGACGCCGAACTCGGTGAACGCGTATGCCTGTTCGTCGTCCCCGCGGGTGGGGCGCGGGTGGACCTGGCAGACGT
GCGTGCCTCGATGCTCGACCGCGGCGTCGCGGCGTTCAAGCTGCCGGACCGACTGGTCAGCGTGGACGCGCTGCCGATGA
CACCACTCGGCAAAATCGACAAAAAGGCATTGCGGGACCAGATCCCGACGCACCTGGACACTGCCTGA

Protein sequence : 
MPPTAVHPTRTGTVPWPAEVATRYLAEGYWAGRPLGAYLTAAARANPAAIALVDGDLRLSYRELMSRADGAAARLVERGV
SGDDRVVVQLPNCWEHIVLTVACLRLGAVPVWALPEHRLREITGVAARAEARVLVVPARHREFDHRAMAHEVAATVPSIE
HVLVTGSADPGEDLGRLCEPAADPAALSARFDAAAPDATAVATFLLSGGTTGTPKLVPRTHNDLAYMVGEAARLCEFGPD
TAYLAALPLGHGFPYTGPGVLGALMSGGRVVIAASPAPGPALATIERERVTATSIVPAIALRWLAHHAAHPGRDLGSLRL
VQIGAARLEPDAAARIEPELGGRLQQVFGMGEGLLCLTRLDDPPAVVHHTQGRPISPADEVLIVDDEDQPVRPGEAGALL
TRGPYTLRGYYRSPEIDAASFLADGWYRTGDIVRQTPDGNLVVTGREKDLINRGGEKVSAVEVEGFALALDGVTQAAAMA
MSDAELGERVCLFVVPAGGARVDLADVRASMLDRGVAAFKLPDRLVSVDALPMTPLGKIDKKALRDQIPTHLDTA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-83 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-83 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-83 44
ybtE CAA21394.1 - Virulence HPI Protein 7e-83 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-83 44
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-83 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-83 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-82 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-82 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Sare_4933 YP_001539671.1 AMP-dependent synthetase and ligase VFG0168 Protein 1e-98 46
Sare_4933 YP_001539671.1 AMP-dependent synthetase and ligase VFG1817 Protein 2e-87 46
Sare_4933 YP_001539671.1 AMP-dependent synthetase and ligase VFG0932 Protein 5e-85 42