Gene Information

Name : lysS (Spea_0757)
Accession : YP_001500619.1
Strain : Shewanella pealeana ATCC 700345
Genome accession: NC_009901
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 939729 - 941234 bp
Length : 1506 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAACAAACCCAAGACGAGAACAAGTTAATTGCAGAGCGTCGTGCTAAATTAGAGCACATTCGTACCAACTGCCC
AGCAAACGGTCACCCGAACAACTTCGATCGAAAACACAAAGCGGCAGATATCCAAGCTGAATTTGGTCACAACACCAAAG
AAGAGCTTGAAGGCATGGGCATTGAGCGTTCAATCGCTGGCCGTGTTATGGCGAAGCGTGGTCCATTCTTGGTGATCCAA
GATGTGAGCGGTCGTATCCAAGCTTATGCGGGTAAAGACGTTCAAAAAGATCTTAAAGAGAAGTTCTTAGGTCTAGATAT
TGGTGACATCATAGGTGTGACCGGTCAGCTACACCTTTCAGGTAAGGGCGACCTATACGTGAACATGGAAGAGTACCAGT
TGCTAACTAAGGCACTGCGTCCACTTCCTGAAAAGTTTCACGGTCTAACTGACCAAGAGACTCGCTACCGTCAGCGTTAT
GTAGACTTGATCGTTAACGAAGACTCTCGTGAAGCCTTTATCATGCGTTCTAAAGTGGTTTCGGCTATCCGTAACTTCAT
GGTGAAGAAAGAGTTCATGGAAGTTGAAACACCTATGATGCACAGCATTCCAGGTGGTGCTTCAGCACGTCCATTCGAGA
CGCATCATAATGCTCTTGATATCGCTATGTACCTACGTATTGCACCTGAGCTATACCTTAAGCGTCTAGTGGTTGGTGGT
TTTGAGCGCGTATTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCTCCTCGCCATAACCCTGAATTCACTATGAT
GGAATTCTATATGGCGTACGCTGACTTCAACGATCTAATGGATCTGACTGAAGAGATGCTAAGCTCTATCGCAACTGAGC
TATGTGGTTCTCCACAGCTTCCATACGGTGAGCACACTGTTGATTTTGGTGGCCCATATGCACGTTTGAGCATGTTGGAT
GCAATCAAGAAGTACAACCCAGACAACGCAACAATCCAGTCTATGACTTATGAAGAAGTTAAAGACGTTGAGTTTATGCG
TGACTTGGCTAAGAGCCTAGGCATGACAGTTGAGAAGTTCTGGACTTGTGGCCAGCTACTAGAAGAGATCTTTGGTGAAA
CAGCTGAAACTCAGTTGATGCAACCTACCTTCATCACTGGCTACCCAGCGGATATCTCTCCACTAGCACGTCGTAACGAC
GACAACCATTTCATCACAGACCGTTTCGAGTTCTTCATCGGCGGCCGTGAAGTGGCTAACGGTTTCTCTGAGCTTAACGA
TGCTGAAGATCAAGACAGCCGTTTCAAGGCTCAGGTTGATGCTAAAGATGCTGGTGATGACGAAGCGATGTTCTATGACG
CTGACTACATCACTGCACTAGAGCACGGCTTACCACCAACTGCGGGTCAAGGTATCGGTATCGACCGTCTAGTGATGCTA
TTCACTAACACGCACACTATCCGTGACGTGATCCTATTCCCTGCGATGCGTCCACAAGCTAACTAA

Protein sequence :
MTEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMGIERSIAGRVMAKRGPFLVIQ
DVSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSREAFIMRSKVVSAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQLPYGEHTVDFGGPYARLSMLD
AIKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKFWTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQAN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-137 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001500619.1 lysyl-tRNA synthetase VFG1668 Protein 5e-137 57