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Name : lysS (BPUM_0066) Accession : YP_001485326.1 Strain : Bacillus pumilus SAFR-032 Genome accession: NC_009848 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 67805 - 69304 bp Length : 1500 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAGTAATGAAGGGCTTAATAACGAAGAATTAAATGACCAATTCCAGGTCAGACGTGACAAAATGAATAAAATGAGAGA AGAAGGTATCGATCCGTTCGGTGAACGATATGACCGTTCTCATCAATCTGCACAAATTATTGCTGAATACGATGAGTTTT CTAAAGAAGACTTAGAAGAAAAGTCTGCACAAGTAACGATCGCTGGACGTATGATGACAAAGCGTGGAAAAGGGAAAGCT GGCTTTGCGCATATCCAAGACTTAGAAGGACAAATCCAAATCTACGTTCGTAAAGATAGTGTAGGAGAAGAAGCTTATGA ATTATTCAAAAGCTCAGACCTAGGCGATATTATCGGTGTAACAGGAACAGTATTTAAAACGAATGTAGGCGAACTTTCTA TTAAAGCGACTAGCTTTGAGGTTCTCACAAAAGCACTTCGCCCGCTTCCTGACAAATATCATGGACTTAAAGATGTCGAA CAACGCTATCGTCAGCGTTACCTTGACCTGATTGTAAATCCAGAAAGTAAGCAGACGTTCATCATGCGAAGCAAGATCAT CCAATCTATGAGAAGATACTTAGATTCTAAAGGATACCTAGAAGTTGAAACACCAACAATGCACAGCATCCCTGGTGGTG CATCAGCACGTCCTTTCATTACTCATCACAATGCACTGGATATGCCACTTTATATGCGTATTGCGATTGAACTTCACTTA AAACGCCTTATTGTCGGTGGTCTAGAGAAGGTATACGAGATTGGTCGTGTATTCCGTAACGAAGGTGTATCAACTCGTCA TAACCCAGAATTCACTATGATCGAGCTTTACGAAGCTTACGCAGATTACAAAGACATCATGAACCTAACTGAAAACCTGA TTGCTCATATTGCCGAAGAGGTACTAGGAACAACCACTATTCAATACGGAGAAGATGAAATTGATCTGAAGCCTGAATGG AAGAGACTCCATATGGTTGAAGCAGTCAAAGAAGCAACTGGTGTAGACTTCTGGCAGGAGATGTCTGTAGAAGAAGCGCA GCAGCATGCAGCTGACCACGGAATTGAAATTACGAAAAATATGACGGTAGGTCATATCATCAATGAGTTCTTCGAGCAAA AAGTGGAGGAAACATTGGTACAGCCTACATTTATTTACGGACATCCAGTGGAGATTTCTCCATTAGCTAAGAAAAATCCT GAAGACCCTCGCTTCACAGATCGCTTTGAGCTATTTATCGTACGCCGTGAGCATGCTAATGCATTCACAGAACTAAACGA TCCAATCGATCAAAGAGAACGATTTGAAGCTCAATTAAAAGAACGTGAAGAAGGAAATGACGAAGCGCATCTAATGGATG ACGATTTTGTTGAAGCATTAGAATATGGTATGCCTCCAACAGGCGGACTAGGAATCGGTATCGACCGGTTAATCATGCTA TTAACAAATTCTCCATCAATCAGAGATGTACTGCTTTTCCCGCAAATGAGAAACCGCTAA Protein sequence : MSNEGLNNEELNDQFQVRRDKMNKMREEGIDPFGERYDRSHQSAQIIAEYDEFSKEDLEEKSAQVTIAGRMMTKRGKGKA GFAHIQDLEGQIQIYVRKDSVGEEAYELFKSSDLGDIIGVTGTVFKTNVGELSIKATSFEVLTKALRPLPDKYHGLKDVE QRYRQRYLDLIVNPESKQTFIMRSKIIQSMRRYLDSKGYLEVETPTMHSIPGGASARPFITHHNALDMPLYMRIAIELHL KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMNLTENLIAHIAEEVLGTTTIQYGEDEIDLKPEW KRLHMVEAVKEATGVDFWQEMSVEEAQQHAADHGIEITKNMTVGHIINEFFEQKVEETLVQPTFIYGHPVEISPLAKKNP EDPRFTDRFELFIVRREHANAFTELNDPIDQRERFEAQLKEREEGNDEAHLMDDDFVEALEYGMPPTGGLGIGIDRLIML LTNSPSIRDVLLFPQMRNR |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-123 | 51 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001485326.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 6e-124 | 51 |
