Gene Information

Name : pyk (BPUM_2561)
Accession : YP_001487781.1
Strain : Bacillus pumilus SAFR-032
Genome accession: NC_009848
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2571262 - 2573022 bp
Length : 1761 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGAGAAAGACAAAAATCGTTTGTACAATCGGTCCAGCAAGTGAAACCATTGAAAAGCTGACTGAATTGATTGAAGCAGG
AATGAATGTAGCCAGACTAAACTTCTCTCATGGAGATTTTGAAGAGCATGGTGCACGCATCGAAAACATTCGTAAAGCAG
GGAAAACATTAGGCAAGGACATTGCGATCCTTCTTGATACAAAAGGTCCAGAGATCCGTACACGTACAGTTGAAAACGGC
TCAATTGAGCTAGTCGCTGGTGCAGATCTCATCGTAAGTATGGAAGACATTGTTGGAAATACGGAAAAAATTAGTGTGAC
ATATGAAGATTTGATTCATGATGTAGAAGTGGGTTCAACAATCCTTTTAGATGATGGTCTCATTGGTTTAGAAGTGAAAG
AACTGAACATGGATGAGAAAGAAATCGTCACAAAGGTCATGAATACAGGCACGCTCAAAAACAAAAAAGGTGTAAACGTA
CCAGGTGTAAGTGTGAACTTGCCAGGTATTACAGAAAAAGATGCCAATGATATTCTGTTCGGAATTGAACAAGGCGTTGA
TTTCATCGCAGCTTCTTTTGTGAGACGAGCTTCTGATGTTCTTGAAATTCGTGAGCTTCTTGAAAAGAATAATGCGGCTG
ACATTCAAATCATCCCTAAGATTGAAAACCAAGAGGGTGTTGATAACATTGATGAAATCCTTGAGGTATCAGACGGTTTG
ATGGTTGCGCGTGGAGATCTTGGTGTAGAGATCCCAGCAGAAGAAGTACCGCTTGTTCAAAAAATGCTCATCAAAAAATG
CAATCGCTTGGGCAAACCTGTCATCACAGCAACCCAAATGCTTGATAGCATGCAGCGTAATCCTCGTCCAACTCGTGCGG
AAGCAAGTGACGTGGCAAATGCTATTTTTGATGGCACAGATGCGATTATGCTTTCTGGTGAAACAGCAGCAGGAACGTAT
CCAGTGGAAGCTGTACAAACAATGCATAACATTGCATCACGTTCAGAAGATGCACTCAACTATAAAGCCATTCTTTCTCG
CAGAAGCGAAGAAGTAGATGTCAGCATTACTGATGCCATTGGTCAATCTGTTGCACATACAGCGATGAAGCTTGACGTTG
CAGCGATTGTGACACCTACGGAAAGCGGTCATACAGCGAGAATGATTTCAAAATACAGACCAAAAGCACCAATCGTAGCA
GTCACAGCAAACGAATCAGTTGCAAGAAAGCTATCTCTTGTATTCGGTGTATTTGCAAAGAGTGGATCAAAAACGACGTC
TACGGATGAAATGCTTGAGAATGCAGTTGAGAAATCGATTGAAACAGGCTACGTGAGACATGGCGATTTAATCGTTATTA
CAGCTGGTGTGCCAGTTGGTGAAACAGGTACAACAAACTTAATGAAAGTGTATGTTGTCGGTGACATCATTGCGAAAGGT
CAAGGAATTGGCCGTAAATCTGCATTTGGACCGGTTGTCATTGCACAAAGCGGAAAAGAAGCAGAAGAAAAAATGTACGA
TGGGGCGATTCTTGTCGCAAACTGTACAGATCGTGATATGATGAGTGCTTTAGAGAAAGCATCTGCCTTGATCACTGAAG
AAGGCGGCTTAACAAGCCATGCAGCAGTCGTTGGACTCAGCCTTGGCATTCCAGTGATTGTAGGTCTTGATGGAGCTACA
ACACTCCTAAAAGAAGGCGAAGAAATTACAGTAGATCCGGCACGCGGAGCGATCTACAAAGGCCGTGCAAGCGTCCTATA
A

Protein sequence :
MRKTKIVCTIGPASETIEKLTELIEAGMNVARLNFSHGDFEEHGARIENIRKAGKTLGKDIAILLDTKGPEIRTRTVENG
SIELVAGADLIVSMEDIVGNTEKISVTYEDLIHDVEVGSTILLDDGLIGLEVKELNMDEKEIVTKVMNTGTLKNKKGVNV
PGVSVNLPGITEKDANDILFGIEQGVDFIAASFVRRASDVLEIRELLEKNNAADIQIIPKIENQEGVDNIDEILEVSDGL
MVARGDLGVEIPAEEVPLVQKMLIKKCNRLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGTY
PVEAVQTMHNIASRSEDALNYKAILSRRSEEVDVSITDAIGQSVAHTAMKLDVAAIVTPTESGHTARMISKYRPKAPIVA
VTANESVARKLSLVFGVFAKSGSKTTSTDEMLENAVEKSIETGYVRHGDLIVITAGVPVGETGTTNLMKVYVVGDIIAKG
QGIGRKSAFGPVVIAQSGKEAEEKMYDGAILVANCTDRDMMSALEKASALITEEGGLTSHAAVVGLSLGIPVIVGLDGAT
TLLKEGEEITVDPARGAIYKGRASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 8e-111 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-111 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-111 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-111 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_001487781.1 pyruvate kinase VFG0479 Protein 1e-111 53