Gene Information

Name : fliI (BPUM_1522)
Accession : YP_001486765.1
Strain : Bacillus pumilus SAFR-032
Genome accession: NC_009848
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1576207 - 1577523 bp
Length : 1317 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAACATCGATGTGTTAAAAGAAACGATTGAAACCGCTGATCCTTATAAACGATTTGGTAAAGTCAATCGAGTTGTTGG
ACTGATGATTGAATCTAAGGGGCCGGAAAGTTCTATCGGAGACGTGTGTCTCATTCATAAAAAGGGACGTAAGCACGATC
CGATAAAAGCAGAGGTTGTAGGTTTTAGAGATGAACATATTCTGCTCATGCCTTATGTAGAAGCATCCAACATTTCACCA
GGAAGTCTAGTTGAAGCGACAGGTGAGTCGCTAAAGGTCAAAGTTGGTACGGGGTTAATTGGCGAAGTGGTTGATGCCTT
CGGTGAACCACTGGACGGCAGTATGCTTCCAAAGGGACTTGCACCTGTTTCCACAGATCAGTCACCTCCAAACCCGATGG
ACAGGCCACCTATTCGCGAAAAAATGGGCGTTGGTGTCAGAGTGATCGACAGCCTGCTTACAGTCGGTACTGGTCAGAGG
ATTGGGATTTTTGCTGGAAGTGGTGTTGGTAAAAGTACCTTAATGGGGATGATTGCGAAGCAGACAGAAGCAGATCTGAA
TGTGATTGCCCTTGTTGGAGAGCGTGGCCGGGAAGTTCGTGAGTTTATTGAAAAGGATTTAGGTACGGAGGGACTAAAGC
GATCGATTGTTGTCGTTGCAACTTCTGACCAGCCTGCACTAACGAGAATGAAGGCTGCTTATACAGCAACAGCTATTGCA
GAATATTTTAGAGACAGAGGAAAAAATGTCATGTTCATGATGGACTCTGTTACACGGGTCGCTATGGCACAAAGGGAAAT
CGGTCTTGCAACTGGTGAACCGCCAACGACAAAAGGATATACGCCTTCAGTGTTCGCTATACTGCCAAAATTACTTGAAC
GAACTGGAACGAATCAGCTTGGTTCCATCACAGCGTTTTACACAGTGCTTGTTGATGGTGACGATATGAATGAGCCAATC
GCTGATACAGTTAGAGGAATCTTAGATGGTCACATTGTACTTGATCGTGCTTTAGCCAACAAAGGACAATTTCCCGCTGT
CAATATTCTGAAAAGTATCAGCCGTGTTATGAGCAATATCGCAAGTCATGATCACAAGGAGGCGGCCAACCGGTTCCGCC
AGCTTTTATCCACTTATCAAAACGCTGAAGACCTCATTAACATTGGAGCATATAAAAAGGGCTCCTCGAAAGACATTGAT
GATGCCATGCAGGCTTATCCTCATCTGATTTCATTTTTGAAGCAGGATGTAGAAGAGGCTGTATCTATTGAAGACAGTGT
GAATGTATTGCTCGGTTTGATGAATAGAGAGGATTGA

Protein sequence :
MNIDVLKETIETADPYKRFGKVNRVVGLMIESKGPESSIGDVCLIHKKGRKHDPIKAEVVGFRDEHILLMPYVEASNISP
GSLVEATGESLKVKVGTGLIGEVVDAFGEPLDGSMLPKGLAPVSTDQSPPNPMDRPPIREKMGVGVRVIDSLLTVGTGQR
IGIFAGSGVGKSTLMGMIAKQTEADLNVIALVGERGREVREFIEKDLGTEGLKRSIVVVATSDQPALTRMKAAYTATAIA
EYFRDRGKNVMFMMDSVTRVAMAQREIGLATGEPPTTKGYTPSVFAILPKLLERTGTNQLGSITAFYTVLVDGDDMNEPI
ADTVRGILDGHIVLDRALANKGQFPAVNILKSISRVMSNIASHDHKEAANRFRQLLSTYQNAEDLINIGAYKKGSSKDID
DAMQAYPHLISFLKQDVEEAVSIEDSVNVLLGLMNRED

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-102 51
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-102 51
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-101 51
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-101 51
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-102 51
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-101 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-101 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-98 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-98 49
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-98 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-92 49
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-82 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-82 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-94 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-94 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-94 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-94 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-94 47
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-85 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-85 46
escN CAI43874.1 EscN protein Virulence LEE Protein 7e-86 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 9e-86 46
escN AAC31513.1 L0034 Virulence LEE Protein 4e-86 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 5e-86 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-86 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 5e-86 46
escN AAC38383.1 EscN Virulence LEE Protein 2e-86 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-86 46
escN AAK26715.1 EscN Virulence LEE Protein 2e-85 46
escN AAL57542.1 EscN Virulence LEE Protein 2e-85 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-86 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 9e-80 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 6e-81 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-81 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-81 44
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-77 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-78 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 9e-86 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-78 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-78 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-78 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-78 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-78 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001486765.1 flagellum-specific ATP synthase VFG2144 Protein 3e-107 53
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0048 Protein 1e-101 51
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0390 Protein 2e-101 50
fliI YP_001486765.1 flagellum-specific ATP synthase VFG1254 Protein 5e-97 49
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0192 Protein 2e-100 48
fliI YP_001486765.1 flagellum-specific ATP synthase VFG2499 Protein 1e-93 48
fliI YP_001486765.1 flagellum-specific ATP synthase VFG2331 Protein 3e-91 47
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0515 Protein 2e-94 47
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0730 Protein 1e-86 46
fliI YP_001486765.1 flagellum-specific ATP synthase VFG0812 Protein 2e-86 46
fliI YP_001486765.1 flagellum-specific ATP synthase VFG1008 Protein 2e-75 43
fliI YP_001486765.1 flagellum-specific ATP synthase VFG2459 Protein 2e-81 42