Gene Information

Name : APECO1_O1CoBM95 (APECO1_O1CoBM95)
Accession : YP_001481250.1
Strain :
Genome accession: NC_009837
Putative virulence/resistance : Unknown
Product : insertion element IS2 transposase InsD
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2801
EC number : -
Position : 80760 - 81665 bp
Length : 906 bp
Strand : +
Note : IS2 transposase; similar to NP_418693; identified by match to protein family HMM PF00665; IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both

DNA sequence : 
GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA
GTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTC
TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAA
CTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACC
TGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTGGCTGTGAAAGAAAGTAATCAGCGATGGTGCTCTGACGGGT
TCGAGTTCCGCTGTGATAACGGAGAAAAACTGCGAGTCACGTTCGCGCTGGACTGCTGTGACCGTGAGGCACTGCACTGG
GCGGTCACTACGGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGA
GCTTCCGGCGTCTCCAGTAGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGGCAGTTTGCCCGGA
TGTTGGGGCTTGAACCGAAGAGCACGGCGGTGCGGAGTCCGGAGAGTAACGGCATAGCAGAGAGCTTCGTGAAAACGATA
AAGCGTGACTACATCAGTGTCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTA
TAACGAATGGCATCCGCATAGTGCACTGGGTTATCGCTCGCCACGGGAATATCTACGGCAGCAAGCCAGTAATGGGTTAA
GTGATAACAGGTGTCTGGAAATATAG

Protein sequence : 
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE
LDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHW
AVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTI
KRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLEI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAC39284.1 hypothetical protein Not tested LPA Protein 7e-122 99
insD YP_002414033.1 insertion element IS2 transposase InsD Not tested Not named Protein 8e-137 98
S4050 NP_839218.2 insertion element IS2 transposase InsD Not tested SHI-2 Protein 7e-137 98
SF3708 NP_709447.1 IS2 transposase TnpB Not tested SHI-2 Protein 7e-137 98
SF3721 NP_709460.1 IS2 transposase TnpB Not tested SHI-2 Protein 7e-137 98
S4061 NP_839230.2 insertion element IS2 transposase InsD Not tested SHI-2 Protein 3e-137 98
tnpF AAD44739.1 TnpF Not tested SHI-2 Protein 3e-119 97
unnamed AAL67379.1 transposase-like protein Not tested PAI II CFT073 Protein 6e-119 97
S3189 NP_838472.2 insertion element IS2 transposase InsD Not tested SHI-1 Protein 6e-136 97
SF2985 NP_708759.1 insertion element IS2 transposase InsD Not tested SHI-1 Protein 4e-117 96
ECO111_3773 YP_003236109.1 putative IS2 transposase InsAB Not tested LEE Protein 2e-122 95
aec49 AAW51732.1 Aec49 Not tested AGI-3 Protein 8e-122 84
unnamed CAC39283.1 hypothetical protein Not tested LPA Protein 2e-84 62
c5197 NP_757045.1 transposase insD Not tested PAI II CFT073 Protein 3e-62 60
insD YP_854263.1 IS2 transposase Not tested PAI I APEC-O1 Protein 4e-66 60
insD AAZ04432.1 IS2 transposase Not tested PAI I APEC-O1 Protein 3e-66 60

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
APECO1_O1CoBM95 YP_001481250.1 insertion element IS2 transposase InsD VFG1732 Protein 3e-137 98
APECO1_O1CoBM95 YP_001481250.1 insertion element IS2 transposase InsD VFG0621 Protein 3e-137 98
APECO1_O1CoBM95 YP_001481250.1 insertion element IS2 transposase InsD VFG0608 Protein 3e-137 98
APECO1_O1CoBM95 YP_001481250.1 insertion element IS2 transposase InsD VFG0649 Protein 2e-117 96