Gene Information

Name : lysS (CKO_04251)
Accession : YP_001455745.1
Strain : Citrobacter koseri ATCC BAA-895
Genome accession: NC_009792
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 3942394 - 3943911 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAACACGCACAGGGCGCTGACGCGGTAACCGATCTTAACAACGAACTGAAAACGCGCCGCGAGAAGCTGGC
TAGCCTGCGCGAGCAGGGTATCCCGTTCCCGAATGATTTCCGTCGCGATCACACCTCAGACCAACTGCACGCTGACTTCG
ACGCGAAAGAGAACGAAGAACTGGAAGCGCTGAACATCGAAGTGTCCGTTGCGGGCCGTATGATGACCCGTCGTATTATG
GGTAAAGCGTCTTTCGTTACGTTGCAGGATGTCGGCGGCCGTATTCAGCTGTACGTTGCCCGTGACGATCTGCCGGAAGG
CGTTTACAACGAGCAGTTCAAAAAATGGGATCTGGGCGATATCCTCGGCGCGAAAGGGAAGCTGTTCAAGACCAAAACCG
GTGAGCTGTCCATCCACTGCACCGAACTGCGTCTGCTGACCAAAGCGCTGCGTCCGCTGCCGGATAAATTCCACGGCTTG
CAGGATCAGGAAGCGCGCTATCGCCAGCGTTACCTGGATCTCATCTCTAACGATGAATCCCGTAACACCTTTAAAGTCCG
TTCACAGATTATGGCGGGTATTCGTCAGTTCATGGTGGGCCGCGGCTTTATGGAAGTGGAAACCCCGATGATGCAGGTGA
TTCCTGGCGGTGCGGCAGCACGTCCGTTTATCACCCATCACAACGCGCTGGATCTGGACATGTACCTGCGTATCGCGCCG
GAACTGTACCTGAAGCGTCTGGTGGTTGGCGGCTTCGAGCGTGTGTTCGAAATCAACCGCAACTTCCGTAACGAAGGGAT
CTCCGTTCGTCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCGTATGCGGACTATAAAGACCTGATCGAACTGA
CCGAATCGCTGTTCCGTACGCTGGCGCAGGATATCCTGGGCAAAACTGAAGTGCCTTACGGCGATGAAGTGTTCGACTTC
GGCAAGCCGTTCGAAAAACTGACCATGCGCGAGGCTATCAAGAAATACCGTCCTGAAACGGATATGGCCGATCTGGATAA
CTTCGACTCCGCCAAAGCGATCGCTGAATCTATCGGCATCAAGGTTGAGAAAAGCTGGGGCCTGGGCCGCATTGTAACTG
AGATCTTCGAAGAAGTGGCGGAAGCGCACCTGATTCAGCCGACCTTCATCACCGAATATCCGGCAGAGGTTTCTCCGCTG
GCGCGCCGTAACGACGTGAACCCGGAAATCACCGACCGCTTTGAATTCTTCATCGGCGGCCGTGAAATCGGTAACGGCTT
TAGCGAGCTGAACGATGCAGAAGATCAGGCGCAGCGTTTCCAGGATCAGGTTAACGCGAAAGAAGCCGGTGACGACGAAG
CGATGTTCTACGACGAAGACTACGTTACCGCTCTGGAGCACGGTCTGCCGCCGACAGCGGGTCTGGGAATTGGTATCGAC
CGCATGGTAATGCTGTTCACCAACAGCCACACCATCCGCGACGTGATTCTGTTCCCGGCGATGCGCCCGGTAAAATAA

Protein sequence :
MSEQHAQGADAVTDLNNELKTRREKLASLREQGIPFPNDFRRDHTSDQLHADFDAKENEELEALNIEVSVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
QDQEARYRQRYLDLISNDESRNTFKVRSQIMAGIRQFMVGRGFMEVETPMMQVIPGGAAARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQDILGKTEVPYGDEVFDF
GKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGIKVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPL
ARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFQDQVNAKEAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-171 69
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-93 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001455745.1 lysyl-tRNA synthetase VFG1668 Protein 3e-171 69