Gene Information

Name : entE (CKO_02567)
Accession : YP_001454112.1
Strain : Citrobacter koseri ATCC BAA-895
Genome accession: NC_009792
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2367767 - 2369380 bp
Length : 1614 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGATGACCATTCCTTTCACCCGTTGGCCGGAGGAGTTTGCCCGCCGCTATCGTGAAAAAGGGTACTGGCAGGATCTGCC
GCTGACCGATATTTTAACCCGCCACGCAGACGGCGATAAAACGGCGGTGATTGATGGCGATCGACACCTGAGCTATCGCG
AACTGAACCAGGCGGCGGATAATCTGGCCTGTAGCCTGCGTCGTCAGGGGATCAAACCCGGCGAAACCGCGCTGGTGCAA
CTGGGCAATGTGGCGGAGTTGTATATCACTTTCTTCGCCCTGCTGAAGCTTGGCGTCGCACCGGTTCTGGCGCTGTTCAG
CCATCAACGGACGGAACTTAACGCTTACGCGAAGCAAATTGAACCGGCGCTGGTGATTGCTGACCGCCAGCACGCGCTGT
TCACCGGGGACGATTTCCTCAATACGTTTATTGCCGAACACAATTCCGTGCGTGTGGTGCTGTTGCTCAACGATACGGGT
GAACACAACCTGCAAACGGCGATTGACCAGCCTGCGGTTGATTTTACCGCCACGCCATCACCTGCCGATGAGGTGGCTTA
TTTCCAGCTTTCCGGCGGTACAACCGGAACGCCGAAGCTGATCCCGCGCACCCATAATGATTATTACTACAGCGTGCGCC
GCAGCAATGAAATTTGCCACTTTACCGAAGCCACGCGTTTCTTATGCGCGATTCCGGCTGCGCATAACTACGCCATGAGT
TCGCCGGGATCGCTGGGTGTCTTTCTGGCCCAGGGAACGGTGGTGCTGGCGGCTGACCCGAGCGCTACGCTCTGTTTCCC
GTTAATTGAAAAGCATCAGATTAATTCAACGGCGCTGGTGCCGCCTGCGGTGAGCCTGTGGTTACAGGCGATTCAGGAGT
GGGGCAGCAACGCGCAACTGGCCTCGTTGAAACTGTTGCAGGTAGGCGGCGCGCGGCTTTCAGCGACGCTTGCCGCGCGT
ATTCCGGCGGAAATCGGCTGTCAGTTGCAGCAGGTATTCGGCATGGCGGAAGGACTGGTGAATTACACCCGTCTGGACGA
CAGCCCGGAGCGCATCATCAATACTCAGGGTTGCCCGATGTGCCCGGACGATGAGGTCTGGGTGGCTGACGCTGACGGCA
ATCCGCTGCCGCAGGGGGAAACGGGACGCCTGATGACGCGCGGGCCGTACACGTTTCGCGGCTATTTTAAAAGCCCGGAG
CATAACGCCAGCGCGTTTGATGCCAACGGCTTTTACTGCTCCGGCGATCTGATCTCCATTGATCCAGATGGCTATATCAC
CGTGCAGGGACGTGAAAAAGATCAGATCAATCGCGGCGGGGAGAAGATCGCCGCTGAAGAGATTGAAAACCTGTTACTGC
GCCACCCGGCCGTAATCCATGCCGCGCTGGTCAGCATGGAAGATGAGCTGATGGGGGAAAAAAGCTGCGCCTGGCTGGTG
GTGAAAGAGCCGCTGCGCGCGGTACAGATACGCCGCTTCCTGCGTGAGCAGGGGGTCGCCGAATTCAAATTGCCGGATCG
CGTGGAGTGCGTCGAGGCGTTGCCTCTGACCCCGGTCGGTAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCAC
GTTCACTGACCTGA

Protein sequence :
MMTIPFTRWPEEFARRYREKGYWQDLPLTDILTRHADGDKTAVIDGDRHLSYRELNQAADNLACSLRRQGIKPGETALVQ
LGNVAELYITFFALLKLGVAPVLALFSHQRTELNAYAKQIEPALVIADRQHALFTGDDFLNTFIAEHNSVRVVLLLNDTG
EHNLQTAIDQPAVDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICHFTEATRFLCAIPAAHNYAMS
SPGSLGVFLAQGTVVLAADPSATLCFPLIEKHQINSTALVPPAVSLWLQAIQEWGSNAQLASLKLLQVGGARLSATLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGCPMCPDDEVWVADADGNPLPQGETGRLMTRGPYTFRGYFKSPE
HNASAFDANGFYCSGDLISIDPDGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAWLV
VKEPLRAVQIRRFLREQGVAEFKLPDRVECVEALPLTPVGKVDKKQLRQWLASRSLT

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
ybtE CAA21394.1 - Virulence HPI Protein 4e-87 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-87 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-87 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-87 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-87 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-87 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-87 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001454112.1 enterobactin synthase subunit E VFG0932 Protein 0.0 91
entE YP_001454112.1 enterobactin synthase subunit E VFG0168 Protein 3e-113 46
entE YP_001454112.1 enterobactin synthase subunit E VFG1817 Protein 3e-86 41