Gene Information

Name : entE (ESA_00797)
Accession : YP_001436910.1
Strain : Cronobacter sakazakii ATCC BAA-894
Genome accession: NC_009778
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 773334 - 774938 bp
Length : 1605 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGACGATCCCTTACACCCGCTGGCCGGACGATCTCGCCGCACGCTACCGCGCCCGCGGCTACTGGCTTGACCTGCCGCT
CACCGATATTTTAAGCCGCCACGCGCAGAGTGCCGCGACAGCGCTTATCGACGGCGAGCGCCGCTACAGCTACCGCGAGC
TGGACGCGGCAGCCACACGTCTTGCGGGCGCGCTGGCGCGTCGCGGGCTTCAGCGTGGCGATACCGCGCTGGTGCAGCTT
GGCAACGTGGCGGAGTTTTATATCGTGTTCTTCGCGCTGCTGAAAATCGGCGTGGCGCCGGTTAATGCGCTGTTCAGCCA
TCAGCGCACCGAGCTTGACGCGTACGCGCGCCAGATTGCACCCGCGCTGCTGATTGCCGACCGCGAGCACGCGCTCTTTA
GCGACGATGCGTATATCACGGCGCTGAAAGCGCAGCATTCGTCGCTGCGCATGACGCTTCTGCGTGGCGACGGGCTGGAC
GCGCTCATTTCAGCGCCGACGACGGATTTCACGCCCACGCCGTCCGCCGCCGATGAAGTGGCGTTTTTCCAGCTTTCCGG
CGGCAGTACCGGCACGCCGAAGCTCATTCCGCGCACGCACAACGACTACTACTACAGCATTCGCCGCAGCGTGGAGATTT
GTGAATTCACCCGCGAAACGCGCTACCTCTGCGCGCTGCCCGCGGCGCACAACTACCCGTTAAGCTCCCCCGGCGCGCTC
GGCGTCTTCTTCGCCGAAGGCTGTGTGGTGCTGGCGCGCGACCCGAGCGCGACGCTCTGCTTCCCGCTTATCGAAGCGCA
CCAGATTAACGTCACCGCGCTGGTGCCGCCCGCCGTCAGCCTCTGGCTACAGGCCATTCAGGAGTGGGGCGACAACCGCC
AGCTGGCGTCGCTGAAACTGTTGCAGGTCGGCGGTGCAAGGCTTTCCGACACGCTCGCCGCGCGCATTCCGGCGGAAATC
GGCTGCCAGCTTCAGCAGGTGTTCGGCATGGCCGAAGGGCTGGTGAATTACACCCGCCTGGACGACCCGGACGAGCGCAT
CTTCACCACCCAGGGGCGACCGATGAGCCCGGATGATGAAGTCTGGGTAGCCGATGAAAACGGCAATCCGCTGCCGCGCG
GTGAGACAGGACGGCTGATGACGCGCGGCCCTTACACCTTCCGCGGTTACTACAACAGCCCTGAACACAACGCCAGCGCT
TTTGATGAGAACGGCTTTTACTGCTCCGGCGATCTGGTGGCCATCGATGAGCAGGGCTACATCACCGTTCAGGGGCGCGA
GAAAGATCAGATAAACCGCGGGGGCGAGAAGATCGCCGCGGAAGAGATCGAGAACCTGCTGCTCGGCCACGAGGCCATTG
TGCACGCGGGGCTGGTTTCGATGGAAGACAGCCTGCTTGGCGAAAAAAGCTGCGCGTATATCGTCACCACCCGCCCGGTC
AAGGCGGTGGAGATCCGCCGTTTTTTACGCGAGCTGGGCGTCGCCGATTTCAAACTGCCGGATCGCGTGGAGCGCGTCGA
TGCGCTGCCGCTCACGCCGGTCGGCAAAGTGGATAAAAAACAACTGCGTCTGTGGCTGGCCGCGCGTCAGCCCGTCTCTG
AGTAA

Protein sequence :
MTIPYTRWPDDLAARYRARGYWLDLPLTDILSRHAQSAATALIDGERRYSYRELDAAATRLAGALARRGLQRGDTALVQL
GNVAEFYIVFFALLKIGVAPVNALFSHQRTELDAYARQIAPALLIADREHALFSDDAYITALKAQHSSLRMTLLRGDGLD
ALISAPTTDFTPTPSAADEVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICEFTRETRYLCALPAAHNYPLSSPGAL
GVFFAEGCVVLARDPSATLCFPLIEAHQINVTALVPPAVSLWLQAIQEWGDNRQLASLKLLQVGGARLSDTLAARIPAEI
GCQLQQVFGMAEGLVNYTRLDDPDERIFTTQGRPMSPDDEVWVADENGNPLPRGETGRLMTRGPYTFRGYYNSPEHNASA
FDENGFYCSGDLVAIDEQGYITVQGREKDQINRGGEKIAAEEIENLLLGHEAIVHAGLVSMEDSLLGEKSCAYIVTTRPV
KAVEIRRFLRELGVADFKLPDRVERVDALPLTPVGKVDKKQLRLWLAARQPVSE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 2e-85 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-85 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-85 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-85 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-85 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-85 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001436910.1 enterobactin synthase subunit E VFG0932 Protein 0.0 76
entE YP_001436910.1 enterobactin synthase subunit E VFG0168 Protein 2e-105 46
entE YP_001436910.1 enterobactin synthase subunit E VFG1817 Protein 4e-81 41