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Name : lysS (ESA_00442) Accession : YP_001436575.1 Strain : Cronobacter sakazakii ATCC BAA-894 Genome accession: NC_009778 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 417306 - 418823 bp Length : 1518 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCTGAACAACAAGCACAAGGCGCAGAAGCGGCGGTCGATCTTAATAACGAACTGAAAACGCGCCGCGAGAAGCTGGC TGCGCTTCGCGAACATGGCGTCGCGTTTCCGAACGATTTTCGTCGCGACCATACCTCAGACCAACTGCACGCTGACTTCG ATGGTAAAGAAAACGAAGAGCTGGAAGCGCTGAACATTGAGGTCTGCGTGGCAGGCCGCATGATGACCCGTCGTATTATG GGTAAAGCGTCTTTCGTTACGCTGCAGGATGTCGGCGGCCGTATTCAGCTGTATGTGGCGCGTGACGATCTGCCGGAAGG CGTTTACAACGAGCAGTTCAAAAAATGGGATCTCGGCGATATCCTGGGCGCGCGCGGTAAACTGTTTAAAACCAAAACCG GCGAGCTTTCCATTCACTGCACCGAGCTGCGTCTGCTCACCAAAGCGCTGCGCCCGCTGCCGGATAAATTCCACGGTCTG CAGGATCAGGAAGCGCGCTACCGCCAGCGTTACCTCGATCTCATCGCGAACGATGAATCCCGCAAAACCTTTAAAGTGCG TTCGCAGATCCTGGCGGGTATCCGTCAGTTCATGGTTGGCCGCGGCTTTATGGAGGTGGAAACCCCGATGATGCAGGTGA TCCCGGGTGGCGCGTCCGCGCGTCCGTTCGTGACGCACCACAACGCGCTGGATCTCGACATGTATCTGCGTATCGCGCCG GAGCTGTACCTCAAGCGTCTGGTCGTGGGCGGTTTCGAGCGCGTGTTTGAGATCAACCGTAACTTCCGTAACGAAGGCAT CTCTGTGCGCCATAACCCTGAGTTCACCATGATGGAACTCTACATGGCGTACGCGGATTACAAAGATCTGATCGAGCTGA CCGAATCCCTGTTCCGTACTCTGGCGCAGGATGTGCTCGGCACAACGCAGGTGCCTTACGGCGAAGAGACGTTTGATTTC GGCAAGCCGTTTGAAAAACTAACCATGCGCGAAGCCATCAAGAAATATCGCCCGGAAACCGATTTGGCGGATCTGGATGA TTTCGAGAAGGCCAAAGGCATTGCGCAGGCAGTCGGCATTAAAGTTGAGAAAAGCTGGGGTCTTGGCCGCGTTGTGACCG AAATCTTCGAGGAAGTGGCCGAAGCGCACCTGATTCAGCCGACCTTCATCACCGAATACCCGGCAGAAGTCTCTCCGCTG GCGCGTCGTAATGATGAAAACCCGGAAATCACCGACCGCTTTGAATTCTTCATTGGTGGCCGCGAAATCGGCAACGGCTT TAGCGAGCTGAACGACGCGGAAGATCAGGCGCAGCGCTTCCAGGATCAGGTTGCCGCGAAAGACGCGGGCGATGACGAAG CGATGTTCTACGACGAAGACTACGTCACCGCGCTGGAGCACGGCTTGCCGCCGACGGCGGGTCTCGGTATCGGGATTGAC CGTATGGTGATGCTGTTTACTAACAGCCACACCATCCGCGACGTTATACTGTTCCCGGCGATGCGTCCGGTGAAATAA Protein sequence : MSEQQAQGAEAAVDLNNELKTRREKLAALREHGVAFPNDFRRDHTSDQLHADFDGKENEELEALNIEVCVAGRMMTRRIM GKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL QDQEARYRQRYLDLIANDESRKTFKVRSQILAGIRQFMVGRGFMEVETPMMQVIPGGASARPFVTHHNALDLDMYLRIAP ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQDVLGTTQVPYGEETFDF GKPFEKLTMREAIKKYRPETDLADLDDFEKAKGIAQAVGIKVEKSWGLGRVVTEIFEEVAEAHLIQPTFITEYPAEVSPL ARRNDENPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFQDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID RMVMLFTNSHTIRDVILFPAMRPVK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 9e-171 | 69 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001436575.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 5e-171 | 69 |
