Gene Information

Name : lysS (CBUD_1642)
Accession : YP_001424981.1
Strain : Coxiella burnetii Dugway 5J108-111
Genome accession: NC_009727
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1637886 - 1639382 bp
Length : 1497 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGAATTAAAAGACCAAATCAAAGAAGAAAATGAACAAATCGCGCAGCGTAAATTAAAATTAAAGAAGCGGCGTGAAGA
GGGTCAAGCTTACCCTAATGATTTTAAGCGCGATAGTTTAGCGGCTGATTTACATGCTGTTTACGATCAATTCGACAGTG
GTGCATTAACCGCAAAGGCTATCCGCGTTAAAATGGCGGGCCGGATGATGACCCGGCGGATTATGGGAAAAGCCAGTTTC
GCCCATATCCAAGACATGAAAGGCCGTATGCAAATTTATGTTACGCGCGATAGTCTGCCCCAAGGCGTCTACTCAGACTT
TAAATCCTGGGATTTGGGAGACATCGTTGGTATTGAAGGAGAGCTTTTTAAAACAAAAACAGAGGAGTTGTCGGTTAAAG
TCGATCAGATTCGTTTATTGACAAAAGCGCTGCGACCAATGCCTGATAAGTTTCACGGACTTCATGATCAAGAACAGCGG
TTTCGTCAGCGCTACCTTGATTTAATTGTCAACGAATCATCCCGTCATTTATTTCAAACTCGCTCGCAAGTTATCGCGCA
AATCCGCCGCTTTCTTGATGATCGCGGCTACATTGAAGTTGAAACGCCGATGATGCATCCATTACCGGGAGGCGCTGCTG
CGCGCCCCTTTGAAACGCACCACAACGCGATGAATATGGATTTATTTTTGCGAATTGCGCCTGAACTTTATTTAAAAAGA
TTAGTCGTCGGTGGCTTTGAAAAGGTGTACGAAATTAACCGTAATTTTCGCAATGAAGGTATTTCAACACGGCATAATCC
CGAATTCACGATGCTCGAGTTTTATCAAGCGTACGCGACTTATGAAGATATGATGATGCTTACCGAATCGATGATTCGCC
ACCTCGCTGAAAAAATTTTTGGCGTTATGGAAATAAAATACCAGGGGGTGAGGATTGATTTAAATAAACCTTTTCCGCGT
TTATCTCTGCGCGATGCGATTTTACAATTTAATCCTGGAATAACGCCCGATCAAATTGATCACCTTGAAACGGCGCGCGA
ATTAGCGCATAAATATGAAATTGCTACCCCCGCTCATTACGGATTAGGGAAAATCCAAACGGAATTGTTTGAAAAACTCG
TAGAAGAAAAGTTGCAACAACCAATTTTTATAACTCATTTTCCAAAAGAAGTGTCGCCCTTATCCCGAGCGAATGAAGAA
AATGACTTTATCACTGATCGTTTTGAGTTTTATGTGGGCGGCCGCGAAATCGCTAACGGCTTTTCCGAATTAAATGATCC
CGAAGATCAAGCCGCTCGCTTTCGCGAGCAATTAAAAGCACGAAATGCTGGGGATTTAGAAGCAATGAGTTTCGATGAAG
ATTATATCACCGCACTAGAATATGGACTTCCCCCGACAGCAGGAGAAGGCATTGGCATCGATCGTTTAGTGATGCTTTTT
ACCGATAACGCCTCCATCCGCGATGTCATTTTGTTCCCCTTATTGCGCTCGAAATGA

Protein sequence :
MELKDQIKEENEQIAQRKLKLKKRREEGQAYPNDFKRDSLAADLHAVYDQFDSGALTAKAIRVKMAGRMMTRRIMGKASF
AHIQDMKGRMQIYVTRDSLPQGVYSDFKSWDLGDIVGIEGELFKTKTEELSVKVDQIRLLTKALRPMPDKFHGLHDQEQR
FRQRYLDLIVNESSRHLFQTRSQVIAQIRRFLDDRGYIEVETPMMHPLPGGAAARPFETHHNAMNMDLFLRIAPELYLKR
LVVGGFEKVYEINRNFRNEGISTRHNPEFTMLEFYQAYATYEDMMMLTESMIRHLAEKIFGVMEIKYQGVRIDLNKPFPR
LSLRDAILQFNPGITPDQIDHLETARELAHKYEIATPAHYGLGKIQTELFEKLVEEKLQQPIFITHFPKEVSPLSRANEE
NDFITDRFEFYVGGREIANGFSELNDPEDQAARFREQLKARNAGDLEAMSFDEDYITALEYGLPPTAGEGIGIDRLVMLF
TDNASIRDVILFPLLRSK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-141 58
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 3e-80 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001424981.1 lysyl-tRNA synthetase VFG1668 Protein 1e-141 58