Gene Information

Name : lysS (RBAM_000920)
Accession : YP_001419761.1
Strain : Bacillus amyloliquefaciens FZB42
Genome accession: NC_009725
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 89354 - 90853 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGTCAAGAAGAGCAAAATCATGAGGAATTGAACGACCAGCTGCAAGTCAGACGAGATAAAATGAACCAGCTGAGAGA
AAACGGCATTGACCCTTTCGGCGAACGTTTCGAGAGAACGCATCAATCTCAACAGATCATTGACGCCTACCAGGAGCTTT
CTAAAGAAGAACTGGAAGAAAAAGCGGTAGAAGTGACCATTGCAGGCCGTATGATGACAAAGCGCGGAAAAGGGAAAGCC
GGCTTTGCGCATCTGCAGGACTTAGAAGGTCAAATTCAAATTTACGTAAGAAAAGACAGTGTCGGCGAAGAACAATATGA
AATCTTTAAATCTTCAGACCTCGGAGACGTTATCGGCATCACGGGGAAAGTTTTCAAAACAAACGTAGGCGAGCTTTCTG
TCAAAGCGACATCTTTTGAACTGCTGACAAAAGCACTGCGTCCTCTTCCTGATAAATATCATGGGTTAAAAGACGTTGAA
CAGCGTTACCGCCAGCGCTATCTCGATCTCATCGTAAACCCTGACAGCAAACAAACGTTTATCACACGCAGCAAAATCAT
CCAAGCGATGAGAAGATACTTAGATGACCATGGATACTTAGAAGTTGAAACACCTACGATGCACAGCATACCGGGAGGAG
CGTCTGCGCGCCCGTTCATCACGCACCATAATGCGCTCGACATGCCTCTTTATATGCGTATTGCGATTGAACTGCACCTG
AAACGCCTGATTGTCGGCGGATTAGAAAAAGTCTACGAAATCGGCCGCGTGTTCCGTAATGAAGGTGTTTCTACACGCCA
TAACCCTGAATTCACGATGATCGAGCTGTATGAAGCGTACGCCGATTATAAAGATATTATGAGCCTTACGGAAAATCTCG
TTGCACATATTGCGCAGGAAGTGCTTGGAAGCACCACAATCCAGTACGGCGAAGAGCAGATTGATCTGAAACCTGAGTGG
AAACGACTCCACATGGTAGATGCGGTCAAAGAAGCGACAGGTGTAGATTTCTGGAATGAGATGACTGTTGAAGAAGCGCG
CGGTCATGCAAAAGAGCATGGTATCGAGATCACAAACTCAATGACGGTGGGTCATATCATCAATGAATTCTTCGAACAAA
AAATTGAAGAAACACTCATTCAGCCTACATTTATTTACGGCCACCCTGTAGAGATTTCTCCTCTTGCTAAGAAAAATCCG
GAGGACCCTCGCTTTACAGACCGTTTTGAGCTGTTTATCGTCGGCCGTGAGCATGCTAATGCGTTTACGGAGCTGAATGA
CCCTATCGACCAGAGGGAACGCTTTGAAGCGCAGTTAAAAGAAAGAGAAGAAGGAAACGATGAAGCGCATATGATGGATG
AAGACTTCGTTGAAGCTCTTGAATACGGCATGCCGCCGACCGGGGGATTGGGTATCGGTATCGACCGCCTTGTCATGCTG
TTAACAAATGCTCCGTCTATCAGAGACGTTTTATTATTCCCTCAAATGAGACAACGCTAG

Protein sequence :
MSQEEQNHEELNDQLQVRRDKMNQLRENGIDPFGERFERTHQSQQIIDAYQELSKEELEEKAVEVTIAGRMMTKRGKGKA
GFAHLQDLEGQIQIYVRKDSVGEEQYEIFKSSDLGDVIGITGKVFKTNVGELSVKATSFELLTKALRPLPDKYHGLKDVE
QRYRQRYLDLIVNPDSKQTFITRSKIIQAMRRYLDDHGYLEVETPTMHSIPGGASARPFITHHNALDMPLYMRIAIELHL
KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMSLTENLVAHIAQEVLGSTTIQYGEEQIDLKPEW
KRLHMVDAVKEATGVDFWNEMTVEEARGHAKEHGIEITNSMTVGHIINEFFEQKIEETLIQPTFIYGHPVEISPLAKKNP
EDPRFTDRFELFIVGREHANAFTELNDPIDQRERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVML
LTNAPSIRDVLLFPQMRQR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-127 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001419761.1 lysyl-tRNA synthetase VFG1668 Protein 6e-128 53