Gene Information

Name : dhbE (RBAM_029030)
Accession : YP_001422465.1
Strain : Bacillus amyloliquefaciens FZB42
Genome accession: NC_009725
Putative virulence/resistance : Virulence
Product : DhbE
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 6.3.2.-
Position : 3029336 - 3030961 bp
Length : 1626 bp
Strand : -
Note : siderophore 2,3-dihydroxybenzoate/bacillibactin synthesis; COG1021, EntE, 2,3-dihydroxybenzoate-AMP ligase

DNA sequence :
GTGCTGACTGGATTTACGCCATGGCCGGAAGAATGGGCTGACAAATACCGAAAAGACGGCTGCTGGAAGGGTGAGACGTT
CGGAGGAATGCTGAAAGAGCGCGCGGCTTTATACGGAAATCAAACTGCCGTGACGTGTAAAGATGTACATTGGACCTACA
AAGAATTAGATGAAAGGGCAGATCGTTTAGCGGCGGGGCTGCATAAGCTCGGAATTCAAAAGGAAGACCGCGTTGTCGTT
CAGCTGCCGAATATCGCTGAGTTTTTTGAAGTGATTTTCGCGCTGTTCCGGCTGGGAGCGCTGCCCGTTTTCGCGCTGCC
TTCTCATAGAAGCAGTGAGATTACGTATTTCTGCGAGTTTGCAGAGGCGAAAGCATACATCATTCCTGATATGCATTCAG
GTTTTGATTACAGGGCGCTTGCCAGACAGGTGAAAGACAAACTTCCGGATTTGGCGCATGTCATTGTGGCCGGCGAAGCT
GAAGAGTTTCAGGAACTGAGCAGCCTGCATAGAGAGCCTGTTCAGCTGGAAGATATAAGCCCGGCGGAAGTCGCCTTTTT
ACAGCTTTCAGGAGGCAGTACGGGGCTGTCCAAGCTGATTCCGCGAACACACGACGATTACATATACAGTTTGCGCATCA
GCGCGGAAATATGCGGCTTGGATCAACACAGCGTTTATTTGGCCGCTCTGCCGACAGCGCATAATTATCCGCTCAGCTCG
CCGGGTGTGCTGGGAACTTTATATGCCGGAGGCCGGGTTGTTCTGTCACCGACGCCGAGCCCGGATGACTGCTTCCCGTT
AATTGAAAAAGAACGTGTCACGATTACAGCGCTCGTCCCGCCGCTTGCGATGGTGTGGATGGACGCATCAGGTTCGCGAA
GTGATGATTTATCAAGCCTTCAAGTGCTGCAGGTGGGAGGCGCCAAATTCAGCGCTGAGGCGGCCCGCCGCGTAAAGAGC
GCTTTCGGATGCACCCTTCAGCAGGTTTTCGGAATGGCGGAAGGATTGGTGAATTATACGAGACTGGATGATCCTGAAGA
GATCATTGTCAATACGCAAGGCAGACCGATGTCACCTTTTGATGAGGTAAGGGTTTTAGACGATGACGGGCGGGATGTCG
CCCCCGGTGAAGCGGGACATTTGTTTACACGCGGCCCTTACACGATTCGCGGATATTATAAAGCGCCTGAACATAATGAA
AGATCATTCACAGCCGACGGATTTTATCAGACCGGCGATATCGTCAAACTGACGGCGGACGGCTATATCATCGTCGAAGG
ACGTGCGAAAGATCAAATCAATCGGGGCGGTGAAAAAATAGCCGCTGAGGAGGTGGAAAATCACCTTCTTGCGCATCCTG
CCGTTCATGATGCGGCCATGGTTTCAATGCCGGATGATTTTCTCGGGGAGCGATCCTGTGTGTTCGTCATCCCAAAAGGA
GAAGCGCCGAAACCGGGAGAGCTGAAAGCATTTTTGCGTGAGCGCGGACTGGCGGCATATAAAATCCCCGACCGGATTGA
ATTTATCGCTTCTTTCCCGAAAACCGGCGTCGGCAAGGTCAGTAAAAAGGATCTTCGCGAAACGATCGCCCAAAAAGTAA
AGCCGGCTGAAATCAAACAATCTTAA

Protein sequence :
MLTGFTPWPEEWADKYRKDGCWKGETFGGMLKERAALYGNQTAVTCKDVHWTYKELDERADRLAAGLHKLGIQKEDRVVV
QLPNIAEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAKAYIIPDMHSGFDYRALARQVKDKLPDLAHVIVAGEA
EEFQELSSLHREPVQLEDISPAEVAFLQLSGGSTGLSKLIPRTHDDYIYSLRISAEICGLDQHSVYLAALPTAHNYPLSS
PGVLGTLYAGGRVVLSPTPSPDDCFPLIEKERVTITALVPPLAMVWMDASGSRSDDLSSLQVLQVGGAKFSAEAARRVKS
AFGCTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGRPMSPFDEVRVLDDDGRDVAPGEAGHLFTRGPYTIRGYYKAPEHNE
RSFTADGFYQTGDIVKLTADGYIIVEGRAKDQINRGGEKIAAEEVENHLLAHPAVHDAAMVSMPDDFLGERSCVFVIPKG
EAPKPGELKAFLRERGLAAYKIPDRIEFIASFPKTGVGKVSKKDLRETIAQKVKPAEIKQS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-120 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-119 51
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-120 51
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 8e-120 51
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-120 51
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-120 51
ybtE CAA21394.1 - Virulence HPI Protein 8e-120 51
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-119 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-119 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
dhbE YP_001422465.1 DhbE VFG0168 Protein 2e-143 55
dhbE YP_001422465.1 DhbE VFG0932 Protein 1e-117 48
dhbE YP_001422465.1 DhbE VFG1817 Protein 1e-113 44